数字农科院2.0

Analysis Of Lncrna-Mediated Gene Regulatory Network Of Bombyx Mori In Response To Bmnpv Infection

文献类型： 外文期刊

作者： Zhang, SL; Yin, HT; Shen, MM; Huang, HL; Hou, QR; Zhang, ZD; Zhao, WG; Guo, XJ; Wu, P

作者机构：

关键词： Bombyx Mori; Lncrna; Lncrna-Mirna-Mrna; Bmnpv; Host-Pathogen Interaction

期刊名称： JOURNAL OF INVERTEBRATE PATHOLOGY

ISSN： 0022-2011

年卷期： 2020 年 170 卷

页码：

收录情况： JCR（2021版）

摘要： Bombyx mori nucleopolyhedrosis virus (BmNPV) has always been a great challenge to the development and stability of the sericulture industry. LncRNAs have been reported to play important roles in gene expression regulation, development and immune response but the roles of lncRNAs in BmNPV infection and silkworm-BmNPV interaction are not clear. We used a genome-wide transcriptome analysis to identify the lncRNAs in Bombyx mori cells (BmN cells) and analyzed the differentially expressed lncRNAs, microRNAs and protein-coding genes in silkworm cells with or without BmNPV infection. A total of 13,159 candidate lncRNAs were identified in the BmN cells, among which 4450 lncRNAs were differentially expressed (DE) with 2837 up-regulated and 1613 down-regulated. In addition, 66 differentially expressed miRNAs (DEmiRNAs) and 7448 differentially expressed mRNAs (DEmRNAs) were identified, and DElncRNA-DEmiRNA-DEmRNA regulatory network was constructed. Gene expression was variable in 4973 of predicted lncRNA cis target genes in BmNPV infected cells. KEGG pathway analysis indicated that the target genes of DElncRNAs are enriched in ubiquitin mediated proteolysis, endocytosis and lysosome pathways. qRT-PCR validated the differential expression of several lncRNAs and miRNAs. Our results suggested that DElncRNAs participate in host response to BmNPV infection via interactions with their target genes and miRNAs. Our results will help us to improve our understanding of lncRNA-mediated regulatory roles in BmNPV infection and provide new insights into silkworm-pathogen interactions.

分类号：

  • 相关文献

[1]Dna Methylomes And Transcriptomes Analysis R.eveal Implication Of Host D na Methylation Machinery In Bmnpv Proliferation In Bombyx Mori. Zhang, Shaolun,Shang, Qi,Zhong, Jinbo,Guo, Xijie,Yin, Haotong,W, Ping,Hou, Qirui,Hou, Qirui,Guo, Xijie,Huang, Haoling,W, Ping,Hou, Qirui,W, Ping. 2019

[2]Knockdown Of Bmtcp-1 Beta Delays B.mnpv Infection In Vitro. Wang, Xue-yang,Shao, Zuo-min,Chen, Qian-ying,Sun, Xia,Sun, Xia,Wang, Xue-yang,Wu, Yang-chun,Xu, Zhen-ping,Xu, Zhen-ping,Wu, Yang-chun,Li, Mu-wang,Li, Mu-wang,Xu, Jia-ping. 2019

[3]Resistant Silkworm Strain Block Viral I.nfection Independent Of Melanization. Chen, TT, Hu, N, Tan, LR, Xiao, Q, Dong, ZQ, Chen, P, Xu, AY, Pan, MH, Lu, C. 2019

[4]Specific Genes Related To Nucleopolyhedrovirus I.n Bombyx Mori Susceptible A nd Near-Isogenic Resistant Strains Through Comparative Transcriptome Analysis. Chen, TT, Tan, LR, Hu, N, Dong, ZQ, Hu, ZG, Qin, Q, Long, JQ, Chen, P, Xu, AY, Pan, MH, Lu, C. 2019

[5]肠炎沙门菌感染鸡lncRNA表达谱分析. 张琪,张锦,王杰,郑炬梅,李庆贺,郑麦青,文杰,赵桂苹. 2021

[6]Study On The Role Of C.ytc In Response To B mnpv Infection In Silkworm, Bombyx Mori (Lepidoptera). Wang, Xue:Yang,Zhang, Guo:Zheng,Li, Mu:Wang,Pang, Hui:Lin,Xu, Ping:Zhen,Wang, Xue:Yang,Zhang, Guo:Zheng,Xu, Ping:Zhen,Wu, Kang:Hui,Li, Mu:Wang. 2019

[7]A H-1 Nmr Based Study O.f Hemolymph Metabonomics In D ifferent Resistant Silkworms, Bombyx Mori (Lepidotera), After Bmnpv Inoculation. Shao, Zuo:min,Wang, Xue:yang,Wu, Yang:chun,Wu, Yang:chun,Zhang, Ying:jian,Wang, Xue:yang,Deng, Ming:jie,Thi Thuy Vu,Xu, Jia:ping,Xu, Jia:ping. 2019

[8]Bmnpv-Mir-415 Up-Regulates The Expression Of T.or2 Via Bmo-Mir-5738. Guo, Xijie,Cao, Xueliang,Zhao, Qiaoling,Qiu, Zhiyong,Huang, Yong,Huang, Yong,Qiu, Zhiyong,Xia, Dingguo,Shen, Xingjia,Xia, Dingguo,Shen, Xingjia,Guo, Xijie,Cao, Xueliang,Zhao, Qiaoling. 2017

[9]Preincubation With A Low Concentration O.f Methyl-Beta-Cyclodextrin Enhances Baculovirus E xpression System Productivity. Shen, Xingjia,Liu, Na,Shen, Xingjia,Hao, Bifang,Huang, Jinshan,Hao, Bifang,Huang, Jinshan. 2019

[10]Metabolic Characterisation Of The Midgut Ofbombyx Morivarieties After Bmnpv Infection Using Gc-Ms-Based Metabolite Profiling. Qian, HY, Li, G, Zhao, GD, Liu, MZ, Xu, AY. 2020

[11]Identification Of Host Proteins Interacting W.ith Toxoplasma Gondii Gra15 ( Tggra15) By Yeast Two-Hybrid System. Liu, Q, Li, FC, Elsheikha, HM, Sun, MM, Zhu, XQ. 2017

[12]Transcriptomic Analysis Reveals Toxoplasma Gondii S.train-Specific Differences In Host C ell Response To Dense Granule Protein Gra15. Liu, Q, Gao, WW, Elsheikha, HM, He, JJ, Li, FC, Yang, WB, Zhu, XQ. 2018

[13]Expression Profiles Of Genes Involved I.n Tlrs And Nlrs S ignaling Pathways Of Water Buffaloes Infected With Fasciola Gigantica. Zhang, FK, Hou, JL, Guo, AJ, Tian, AL, Sheng, ZA, Zheng, WB, Huang, WY, Elsheikha, HM, Zhu, XQ. 2018

[14]Identification Of Two Novel Host P.roteins Interacting With Toxoplasma G ondii 14-3-3 Protein By Yeast Two-Hybrid System. Li, FC, Liu, Q, Elsheikha, HM, Yang, WB, Hou, JL, Zhu, XQ. 2018

[15]Functional Characterization Of Dense Granule P.roteins In Toxoplasma Gondii R h Strain Using Crispr-Cas9 System. Bai, MJ, Wang, JL, Elsheikha, HM, Liang, QL, Chen, K, Nie, LB, Zhu, XQ. 2018

[16]A Unique Combination Of Glycoside Hydrolases In Streptococcus Suis Specifically And Sequentially Acts On Host-Derived Alpha Gal-Epitope Glycans. Chen, P, Liu, R, Huang, MM, Zhu, JL, Wei, D, Castellino, FJ, Dang, GH, Xie, F, Li, G, Cui, ZY, Liu, SG, Zhang, YL. 2020

[17]Hepatic Metabolomics Investigation In Acute A.nd Chronic Murine Toxoplasmosis. Chen, XQ, Elsheikha, HM, Hu, RS, Hu, GX, Guo, SL, Zhou, CX, Zhu, XQ. 2018

[18]Complex And Dynamic Transcriptional Changes A.llow The Helminth Fasciola G igantica To Adjust To Its Intermediate Snail And Definitive Mammalian Hosts. Zhang, XX, Cwiklinski, K, Hu, RS, Zheng, WB, Sheng, ZA, Zhang, FK, Elsheikha, HM, Dalton, JP, Zhu, XQ. 2019

[19]De Novo Transcriptome Sequencing And A.nalysis Of The Juvenile A nd Adult Stages Of Fasciola Gigantica. Zhang, XX, Cong, W, Elsheikha, HM, Liu, GH, Ma, JG, Huang, WY, Zhao, Q, Zhu, XQ. 2017

[20]The Retroviral Accessory Proteins S2, N.ef, And Glycoma Use S imilar Mechanisms For Antagonizing The Host Restriction Factor Serinc5. Ahmad, I, Li, SN, Li, RR, Chai, QQ, Zhang, LX, Wang, B, Yu, CQ, Zheng, YH. 2019

作者其他论文 更多>>