数字农科院2.0

Honeybee (Apis mellifera ligustica) drone embryo proteomes

文献类型： 外文期刊

作者： Li, Jianke;Fang, Yu;Zhang, Lan;Begna, Desalegn

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关键词： Honeybee Drone Embryo 2-DE MALDI-TOF/MS LC-Chip/ESI-QTOF

期刊名称： JOURNAL OF INSECT PHYSIOLOGY

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收录情况： SCI

摘要： Display Omitted a- Our data provided a first preliminary proteome map of honeybee drone embryos. a- More than 100 proteins were successfully identified and 62 of them altered expressions at embryonic ages of 24, 48 and 72h. a- A significant number of proteins were up-regulated at age 48h, a peak time at which carbohydrate and energy metabolism-related proteins are required the most. a- Protein involving in the cytoskeleton, antioxidants and protein folding were highly expressed at 48 and 72h to maintain cell shape, or remove ROS or facilitate protein folding for the developing embryos. a- The biological interaction network and q-PCR validation results provide valuable information for geneticists to specify a precise embryonic age at which to select a suitable protein target for future manipulation of honeybee male stem cells for better pollination services or increased bee product yield. Little attention has been paid to the drone honeybee (Apis mellifera ligustica) which is a haploid individual carrying only the set of alleles that it inherits from its mother. Molecular mechanisms underlying drone embryogenesis are poorly understood. This study evaluated protein expression profiles of drone embryogenesis at embryonic ages of 24, 48 and 72h. More than 100 reproducible proteins were analyzed by mass spectrometry on 2D electrophoresis gels. Sixty-two proteins were significantly changed at the selected three experimental age points. Expression of the metabolic energy requirement-related protein peaked at the embryonic age of 48h, whereas development and metabolizing amino acid-related proteins expressed optimally at 72h. Cytoskeleton, protein folding and antioxidant-related proteins were highly expressed at 48 and 72h. Protein networks of the identified proteins were constructed and protein expressions were validated at the transcription level. This first proteomic study of drone embryogenesis in the honeybee may provide geneticists an exact timetable and candidate protein outline for further manipulations of drone stem cells.

分类号： Q965

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