数字农科院2.0

Visual toxicity of imidacloprid in zebrafish: Unraveling the pathways from ocular morphology to behavior via phototransduction dysregulation

文献类型： 外文期刊

作者： Fu, Ruiqiang;Zheng, Hanfei;Gao, Han;Mao, Liangang;Wu, Chi;Zhu, Lizhen;Liu, Xingang;Zhang, Lan

作者机构：

关键词： Imidacloprid;Visual behavior;Phototransduction;Zebrafish;Ecological health

期刊名称： JOURNAL OF HAZARDOUS MATERIALS

ISSN： 0304-3894

年卷期： 2025 年 501 卷

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： Imidacloprid (IMI) poses risks to aquatic ecosystems and exhibits visual toxicity to nontarget organisms. However, the underlying mechanism of its visual toxicity has not been fully elucidated. After exposure to IMI at the concentrations 10, 100, and 1000 mu g/L for 4 or 21 days, the visual behavior of zebrafish larvae was first suppressed, followed by enhanced locomotor and visual activities as exposure prolonged. The eye development in zebrafish larvae was significantly disrupted. Structurally, the lens area of zebrafish larvae was reduced from 15.63 % to 50.21 % (4 days) and from 37.00 % to 47.22 % (21 days), and the retina exhibited a thickening of the OPL and IPL, along with thinning of the RPE. Genes related to lens structure (e.g., CRYBA4 and CRYBB2) were significantly down-regulated at 4 days (100 and 1000 mu g/L) and then up-regulated (10 and 100 mu g/L) at 21 days at the mRNA and protein levels (P < 0.05). Furthermore, IMI exposure disrupted the phototransduction and retinol metabolism cascade in zebrafish larvae, which involves the generation of visual signals across three critical stages, namely, light input, photopigment activation, and photoelectric conversion. Generally, the key genes and metabolites associated with these processes (e.g., Rhodopsin, OPN1SW1, GNGT1, REP65A, 11-cisretinal) were significantly decreased in zebrafish exposed to IMI for 4 days and increased in those exposed for 21 days (P < 0.05). Therefore, the abnormal color perception was observed in exposed zebrafish larvae. This study elucidates the mechanism underlying IMI-induced visual toxicity in zebrafish and provides crucial theoretical insights for assessing the visual toxicity of agrochemicals.

分类号：

  • 相关文献

[1]Biochemical mechanisms of imidacloprid resistance in Nilaparvata lugens: Over-expression of cytochrome P450 CYP6AY1. Ding, Zhiping,Wen, Yucong,Zhang, Yixi,Liu, Zewen,Han, Zhaojun,Yang, Baojun,Liu, Shuhua.

[2]Bt Toxin Cry1Ie Causes No Negative Effects on Survival, Pollen Consumption, or Olfactory Learning in Worker Honey Bees (Hymenoptera: Apidae). Diao, Qing-Yun,Jia, Hui-Ru,Geng, Li-Li.

[3]E-beta-farnesene synergizes the influence of an insecticide to improve control of cabbage aphids in China. Dong, Jie,Liu, Ying-Jie,Liu, Yong,Heuskin, Stephanie,Lognay, Georges,Chen, Ju-Lian,Bragard, Claude,Tooker, John F.. 2012

[4]Field-evolved resistance to insecticides in the invasive western flower thrips Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae) in China. Gong, Ya-Jun,Jin, Gui-Hua,Li, Bing-Yan,Chen, Jin-Cui,Kang, Zong-Jiang,Zhu, Liang,Wei, Shu-Jun,Gao, Yu-Lin,Reitz, Stuart.

[5]Bt Cry1Ie Toxin Does Not Impact the Survival and Pollen Consumption of Chinese Honey Bees, Apis cerana cerana (Hymenoptera, Apidae). Hou, Chun-Sheng,Diao, Qing-Yun,Jia, Hui-Ru,Geng, Li-Li,Jack, Cameron J.,Ellis, James D.,Liu, Feng.

[6]Mechanisms of imidacloprid resistance in Nilaparvata lugens by molecular modeling. Liu, Gen Yan,Miao, Wei,Ju, Xiu Lian,Liu, Gen Yan,Ju, Xiu Lian. 2010

[7]Comparative and combined acute toxicity of butachlor, imidacloprid and chlorpyrifos on earthworm, Eisenia fetida. Chen, Chen,Qian, Yongzhong,Wang, Yanhua,Zhao, Xueping,Wang, Qiang.

[8]Biodegradation of neonicotinoid insecticide, imidacloprid by restriction enzyme mediated integration (REMI) generated Trichoderma mutants. He, Xiangfeng,Wubie, Abebe Jenberie,Diao, Qingyun,Li, Wei,Xue, Fei,Guo, Zhanbo,Zhou, Ting,Xu, Shufa,Wubie, Abebe Jenberie.

[9]Toxicity of mixtures of lambda-cyhalothrin, imidacloprid and cadmium on the earthworm Eisenia fetida by combination index (CI)-isobologram method. Wang, Yanhua,Zhao, Xueping,Wang, Qiang,Chen, Chen,Qian, Yongzhong,Kong, Xiangzhen.

[10]Cycloxaprid: A novel cis-nitromethylene neonicotinoid insecticide to control imidacloprid-resistant cotton aphid (Aphis gossypii). Qi, Haoliang,Yang, Daibin,Yuan, Huizhu,Rui, Changhui,Qi, Haoliang.

[11]Sublethal effects of imidacloprid on Bemisia tabaci (Hemiptera: Aleyrodidae) under laboratory conditions. He, Yuxian,Wu, Kongming,He, Yuxian,Zhao, Jianwei,Wu, Dongdong,Wyckhuys, Kris A. G..

[12]Use of an innovative T-tube maze assay and the proboscis extension response assay to assess sublethal effects of GM products and pesticides on learning capacity of the honey bee Apis mellifera L.. Peng Han,Chang-Ying Niu,Chao-Liang Lei,Jin-Jie Cui,Nicolas Desneux. 2010

[13]Programmed Cell Death in the Honey Bee (Apis mellifera) (Hymenoptera: Apidae) Worker Brain Induced by Imidacloprid. Wu, Yan-Yan,Zhou, Ting,Wang, Qiang,Dai, Ping-Li,Xu, Shu-Fa,Jia, Hui-Ru,Wang, Xing.

[14]Two cytochrome P450 genes are involved in imidacloprid resistance in field populations of the whitefly, Bemisia tabaci, in China. Yang, Xin,Zhou, Xiaomao,Yang, Xin,Xie, Wen,Wang, Shao-li,Wu, Qing-jun,Pan, Hui-peng,Li, Ru-mei,Yang, Ni-na,Liu, Bai-ming,Xu, Bao-yun,Zhang, You-jun. 2013

[15]Over-expression of CP9 and CP83 increases whitefly cell cuticle thickness leading to imidacloprid resistance. He C.,Liang J.,Yang J.,Xue H.,Huang M.,Fu B.,Wei X.,Liu S.,Du T.,Ji Y.,Yin C.,Gong P.,Hu J.,Du H.,Zhang R.,Xie W.,Wang S.,Wu Q.,Zhou X.,Yang X.,Zhang Y.. 2023

[16]Molecularly Imprinted Polymer On Graphene S.urface For Selective And S ensitive Electrochemical Sensing Imidacloprid. Zhang, M, Zhao, HT, Xie, TJ, Yang, X, Dong, AJ, Zhang, H, Wang, J, Wang, ZY. 2017

[17]Combined ecotoxicological effects of different-sized polyethylene microplastics and imidacloprid on the earthworms (Eisenia fetida). Fu H.,Zhu L.,Mao L.,Zhang L.,Zhang Y.,Chang Y.,Liu X.,Jiang H.. 2023

[18]Integrated non-targeted lipidomics and metabolomics analyses for fluctuations of neonicotinoids imidacloprid and acetamiprid on Neuro-2a cells. Xinlu Wang,Jing Qiu,Yanyang Xu,Guangqin Liao,Qi Jia,Yecan Pan,Tiancai Wang,Yongzhong Qian. 2021

[19]Comparison of Chemiluminescence Enzyme Immunoassay (Cl-ELISA) with Colorimetric Enzyme Immunoassay (Co-ELISA) for Imidacloprid Detection in Vegetables. Rongqi Zhai,Ge Chen,Guangyang Liu,Xiaodong Huang,Xiaomin Xu,Lingyun Li,Yanguo Zhang,Donghui Xu,A. M. Abd El-Aty. 2023

[20]20E biosynthesis gene CYP306A1 confers resistance to imidacloprid in the nymph stage of Bemisia tabaci by detoxification metabolism. Liu, Shaonan,Fu, Buli,Zhang, Chengjia,He, Chao,Gong, Peipan,Huang, Mingjiao,Du, Tianhua,Liang, Jinjin,Wei, Xuegao,Yang, Jing,Yin, Cheng,Ji, Yao,Xue, Hu,Hu, Jinyu,Wang, Chao,Zhang, Rong,Du, He,Yang, Xin,Zhang, Youjun. 2023

作者其他论文 更多>>