数字农科院2.0

Polymorphism of GDF9 gene and its association with litter size in goats

文献类型： 外文期刊

作者： Chu, M. X.;Feng, T.;Cao, G. L.;Fang, L.;Di, R.;Huang, D. W.;Wu, Z. H.;Li, X. W.;Li, N.

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关键词： litter size;genotype frequency;breeding prolificacy

期刊名称： VETERINARY RESEARCH COMMUNICATIONS

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年卷期：

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收录情况： SCI

摘要： Growth differentiation factor 9 (GDF9) was studied as a candidate gene for high prolificacy in goats. The polymorphism of exon 1 and flanking of GDF9 gene was detected by PCR-SSCP in five goat breeds with different prolificacy. Three genotypes (AA, AB and BB) were detected in goat breeds joined and two silent mutations (c.183A > C and c.336C > T) were identified in comparison genotype AA with genotype BB. Heterozygous genotype AB and wild type BB were detected in all five goat breeds and homozygous genotype AA was only detected in Jining Grey goats. The frequencies of genotypes AA, AB and BB were 0.18, 0.42 and 0.40 in Jining Grey goats, respectively. The genotype distribution was different (P < 0.01) between high prolificacy breed (Jining Grey goat) and low prolificacy breeds (Boer, Wendeng Dairy, Liaoning Cashmere and Beijing native goats). The Jining Grey goat does with genotype AA and AB had 0.72 (P < 0.01) and 0.56 (P < 0.01) kids more than those with genotype BB, respectively. The does with genotype AA had 0.16 (P > 0.05) kids more than those with genotype AB. These indicated that the allele A may have certain correlation with prolificacy in Jining Grey goats.

分类号： S85

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