数字农科院2.0

Molecular cloning, tissue expression pattern, and copy number variation of porcine SCUBE3

文献类型： 外文期刊

作者： Liu, X.;Wang, L. G.;Zhang, L. C.;Yan, H.;Zhao, K. B.;Liang, J.;Li, N.;Pu, L.;Zhang, T.;Wang, L. X.

作者机构：

关键词： Cloning;Copy number variation;mRNA expression;Porcine;Signal peptide CUB EGF-like domain-containing protein 3 gene

期刊名称： GENETICS AND MOLECULAR RESEARCH

ISSN： 1676-5680

年卷期： 2016 年 15 卷 1 期

页码：

收录情况： SCI

摘要： The signal peptide CUB EGF-like domain-containing protein 3 (SCUBE3) gene is a member of SCUBE gene family and plays important roles in bone cell biology and the determination of limb bone length. In this study, the full-length transcript of porcine SCUBE3 was cloned using reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends. The full-length sequence of porcine SCUBE3 cDNA was 4131 base pairs and included 21 exons. The SCUBE3 gene contained a 2895-base pair open reading frame that encoded a peptide of 965 amino acids. Comparison of the deduced amino acid sequences of porcine SCUBE3 with those of human, mouse, zebrafish, and rat showed 96, 95, 73, and 95% identities, respectively. Porcine SCUBE3 mRNA expression levels were highest in the backfat, bone marrow, and cartilage tissues. Copy number variation was detected in porcine SCUBE3 and validated by real-time quantitative polymerase chain reaction. Different copy number variations were present in randomly selected individuals and may, therefore, be a good marker for identifying phenotypic traits. Our findings provide a basis for further investigation of the functions and regulatory mechanisms of SCUBE3 in pigs.

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