数字农科院2.0

Corn seeds as bioreactors for the production of phytase in the feed industry

文献类型: 外文期刊

作者: Chen, Rumei;Zhang, Chunyi;Yang, Wenzhu;Zhou, Xiaojin;Sun, Cheng;Chen, Ping;Fan, Yunliu;Chen, Rumei;Zhang, Chunyi;Xue, Guangxing;Yang, Wenzhu;Zhou, Xiaojin;Sun, Cheng;Chen, Ping;Fan, Yunliu;Yao, Bin;Zhang, Junmin

作者机构:

关键词: Phytase;Corn endosperm;Phosphate;Phytate;Animal feed

期刊名称: JOURNAL OF BIOTECHNOLOGY

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Corn seed is a major ingredient of animal feed worldwide. However, it contains phytate, a major phosphate storage form that is unavailable to monogastric animals like pigs and poultry. We report a transgenic corn with bioavailable phosphate, achieved by seed-specific overexpression of Aspergillus niger phytase, an enzyme catalyzing the release of phosphate from phytate. We obtained maximal phytase activity of 125 FTU/g kernels, 1000-fold above that of the wild type, with 1000g of kernels containing up to 67 times the feed industry requirement. Enzymatic characterization of Zea mays recombinant phytase (ZmrPhy) showed it to be equivalent to yeast (Pichia pastoris) recombinant phytase (PprPhy), a commercially available phytase product. An animal feeding trial demonstrated that ZmrPhy had similar nutritional effects on broiler chickens to PprPhy in terms of reducing inorganic phosphorus addition to feed and phosphate excretion in animal manure. These results suggest that transgenic phytase corn can be used directly in the feed industry. Experiments were conducted to assess the food safety of the corn; the results demonstrated no difference versus regular corn. This is the first genetically modified corn officially issued with a biosafety certificate in China and has great potential in the animal feed industry

分类号: Q81

  • 相关文献

[1]A novel phytase gene appA from Buttiauxella sp GC21 isolated from grass carp intestine. Shi, Pengjun,Huang, Huoqing,Wang, Yaru,Luo, Huiying,Wu, Bo,Meng, Kun,Yang, Peilong,Yao, Bin. 2008

[2]Construction of transgenic Bacillus mucilaginosus strain with improved phytase secretion. Li, X,Yang, SH,Yu, XC,Jin, ZX,Li, WD,Li, L,Li, J,Li, MG.

[3]Growth promoting effect of a transgenic Bacillus mucilaginosus on tobacco planting. Li, Xin,Wu, Zhiqiang,Li, Weidong,Yan, Ruixiang,Li, Li,Li, Jun,Li, Yihang,Li, Minggang.

[4]Effects of zinc source and phytate on zinc absorption by in situ ligated intestinal loops of broilers. Yu, Y.,Lu, L.,Wang, R. L.,Luo, X. G.,Liu, B.,Yu, Y.,Lu, L.,Wang, R. L.,Luo, X. G.,Liu, B.,Yu, Y.,Xi, L.. 2010

[5]Transgenic maize plants expressing a fungal phytase gene. Chen, Rumei,Chen, Ping,Yang, Wenzhu,Ma, Qianli,Fan, Yunliu,Xue, Guangxing,Yao, Bin,Zhao, Zuoyu,Tarczynski, Mitchell C.,Shi, Jinrui. 2008

[6]Biochemical characterization of purified phytase produced from Aspergillus awamori AFE1 associated with the gastrointestinal tract of longhorn beetle (Cerambycidae latreille). Lawal, Olusola T.,Onuegbu, Christian,Afe, Ayoola E.,Olopoda, Isaac A.,Igbe, Festus O.,Ojo, Funmillayo M.,Sanni, David M.. 2024

[7]Variations in Cadmium and Lead Bioaccessibility in Wheat Cultivars and Their Correlations with Nutrient Components. Liu, Chang,Xu, Yingming,Wang, Lin,Huang, Qingqing,Yan, Xiuxiu,Sun, Yuebing,Qin, Xu,Liang, Xuefeng. 2024

[8]Determination of Phenylethanolamine A in Animal Feeds Using Ultra High Performance Liquid Chromatography-Photodiode Array Detector. Wang Rui-Guo,Su Xiao-Ou,Wang Pei-Long,Li Yang,Fan Yu-Fei. 2013

[9]Study on the method of using ICP-MS to determine microelements in the animal feed. Wang Pei-long,Su Xiao-ou,Gao Sheng,Wang Tong,Zhu Ruo-hua. 2007

[10]Carry-over of melamine from feed to eggs and body tissues of laying hens. Dong, X. F.,Liu, S. Y.,Tong, J. M.,Zhang, Q.. 2010

[11]A MONOCLONAL ANTIBODY-BASED INDIRECT COMPETITIVE ENZYME-LINKED IMMUNOSORBENT ASSAY FOR THE DETERMINATION OF OLAQUINDOX IN ANIMAL FEED. Wang, Lei,Zhang, Jingyan,Cui, Dongan,Wang, Xuezhi,Yang, Zhiqiang,Zhang, Kai,Qin, Zhe,Meng, Jiaren,Hao, Guijuan,Li, Jianxi.

[12]Purification, gene cloning and characterization of an acidic beta-1,4-glucanase from Phialophora sp G5 with potential applications in the brewing and feed industries. Zhao, Junqi,Shi, Pengjun,Yuan, Tiezheng,Huang, Huoqing,Li, Zhongyuan,Meng, Kun,Yang, Peilong,Yao, Bin. 2012

[13]Influence of mannan oligosaccharide, Ligustrum lucidum and Schisandra chinensis on parameters of antioxidative and immunological status of broilers. Ma, Deying,Li, Qundao,Du, Juan,Liu, Yuqin,Liu, Shengwang,Shan, Anshan.

[14]Concentrations And Congener Group Profiles O.f Short-And Medium-Chain Chlorinated P araffins In Animal Feed Materials. Dong, SJ, Li, XM, Su, XO, Wang, PL. 2019

[15]C-13 NMR detection of non-protein nitrogen substance adulteration in animal feed. Zhao, Chengxiang,Wang, Tongtong,Chen, Furong,Sun, Yongyue,Chen, Gang. 2022

[16]Pilot study on the effect of secondary copper smelters on polychlorinated naphthalene contamination in surrounding agricultural areas. Dong S.,Zhang S.,Zou Y.,Li T.,Wang R.,Wang Y.,Zhao Y.,Cheng J.,Wu G.,Wang P.. 2022

[17]Thermal transition and decomposition properties of pH- and phosphate-induced defatted soybean meals. Zhang, Jinchuang,Wei, Yimin,Zhang, Bo,Su, Xiaofang,Ying, Danyang,Li, Shujing.

[18]Is the effect of silicon on rice uptake of arsenate (As-v) related to internal silicon concentrations, iron plaque and phosphate nutrition?. Guo, W.,Zhu, Y.-G.,Liu, W.-J.,Liang, Y.-C.,Geng, C.-N.,Wang, S.-G.. 2007

[19]Kinetic and isotherms studies of phosphorus adsorption onto natural riparian wetland sediments: linear and non-linear methods. Zhang, Liang,Du, Chao,Du, Yun,Xu, Meng,Chen, Shijian,Liu, Hongbin.

[20]Cultivation of Microalgae in Dairy Farm Wastewater Without Sterilization. Ding, Jinfeng,Zhao, Fengmin,Cao, Youfu,Xing, Li,Liu, Wei,Mei, Shuai,Li, Shujun.

作者其他论文 更多>>