数字农科院2.0

Identification of a novel B-cell epitope specific for avian leukosis virus subgroup J gp85 protein

文献类型： 外文期刊

作者： Li, Xiaofei;Wang, Qi;Gao, Yanni;Qi, Xiaole;Wang, Yongqiang;Gao, Honglei;Gao, Yulong;Wang, Xiaomei;Zhu, Haibo;Sun, Jiashan;Sun, Jiashan;Wang, Xiaomei

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期刊名称： ARCHIVES OF VIROLOGY

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收录情况： SCI

摘要： Avian leukosis virus subgroup J (ALV-J) is an avian oncogenic retrovirus that has caused severe economic losses in China. Gp85 protein is the main envelope protein and the most variable structural protein of ALV-J. It is also involved in virus neutralization. In this study, a specific monoclonal antibody, 4A3, was produced against the ALV-J gp85 protein. Immunofluorescence assays showed that 4A3 could react with different strains of ALV-J, including the British prototype isolate HPRS103, the American strains, an early Chinese broiler isolate, and layer isolates. A linear epitope on the gp85 protein was identified using a series of partially overlapping fragments spanning the gp85-encoding gene and subjecting them to western blot analysis. The results indicated that (134)AEAELRDFI(142) was the minimal linear epitope that could be recognized by mAb 4A3. Enzyme-linked immunosorbent assay (ELISA) revealed that chicken anti-ALV-J sera and mouse anti-ALV-J gp85 sera could also recognize the minimal linear epitope. Alignment analysis of amino acid sequences indicated that the epitope was highly conserved among 34 ALV-J strains. Furthermore, the epitope was not conserved among subgroup A and B of avian leukosis virus (ALV). Taken together, the mAb and the identified epitope may provide valuable tools for the development of new diagnostic methods for ALV-J.

分类号： R37

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