数字农科院2.0

Determination of flavonol glycosides in green tea, oolong tea and black tea by UHPLC compared to HPLC

文献类型： 外文期刊

作者： Jiang, Heyuan;Jiang, Heyuan;Engelhardt, Ulrich H.;Thraene, Claudia;Maiwald, Beate;Stark, Janina;Jiang, Heyuan

作者机构：

关键词： Flavonol glycosides;Green tea;Oolong tea;Black tea;UHPLC;HPLC

期刊名称： FOOD CHEMISTRY

ISSN：

年卷期：

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收录情况： SCI

摘要： An UHPLC method for the determination of flavonol glycosides (FOG) from green and oolong tea vs. black tea has been developed for the first time. Sample clean-up method by means of polyamide column chromatography was optimized with multiple-step elution. Using UHPLC and HPLC with gradient elution and photodiode array detection, eighteen FOG compounds were determined with the aid of electrospray tandem mass spectrometry. These FOG compounds were qualified on both UHPLC and HPLC, and this UHPLC method successfully separated rutin (quercetin-3-O-rutinoside) and K-grg (kaempferol-3-O-glucorhamnoglucoside) while conventional HPLC method did not. The total amounts of FOG compounds in the tea samples were 2.32-5.67 g/kg dry weight (calculated as aglycones), and there is no significant difference for the total FOG content among green tea, oolong tea and black tea. However, kaempferol glycosides are more abundant in green teas, while oolong tea has more quercetin and myricetin glycosides. In black tea quercetin glycosides were most abundant. (C) 2015 Elsevier Ltd. All rights reserved.

分类号： TS2`TS201.2

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