Biological control of Botrytis cinerea on tomato plants using Streptomyces ahygroscopicus strain CK-15
文献类型： 外文期刊
作者： Cheng, Y.;Liu, Y.;Liu, B. H.;Zhang, K. C.
作者机构：
关键词： biological fungicides;Botrytis cinerea;real-time PCR;Streptomyces;wuyiencin
期刊名称： LETTERS IN APPLIED MICROBIOLOGY
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年卷期：
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收录情况： SCI
摘要： We developed a real-time PCR assay to specifically detect and quantify the efficacy of a biological fungicide from Streptomyces ahygroscopicus var. wuyiensis on tomato leaves. This fungicide, the natural secondary metabolite wuyiencin, is an antifungal agent against Botrytis cinerea. Specific primers were designed based on the beta-actin gene sequences, which were used to detect a 303 bp fragment from B. cinerea isolates. Our assay is highly sensitive and can be used to reliably detect and quantify as little as 1.75 pg of B. cinerea DNA. We used this detection method to monitor the progression of B. cinerea infection in inoculated plant material under preventive (wuyiencin) and nonpreventive treatment. After 5 days, plants under preventive treatment exhibited a sharp decrease in fungal biomass and no symptoms, whereas plants under nonpreventive treatment displayed severe disease symptoms. The results demonstrate that wuyiencin has significant effects on B. cinerea in tomato plants and that real-time PCR is a reliable method for evaluating the effects of Streptomyces wuyiensis CK-15 on B. cinerea.
分类号： Q939
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