数字农科院2.0

Identification of a linear B-cell epitope on the avian leukosis virus P27 protein using monoclonal antibodies

文献类型： 外文期刊

作者： Li, Xiaofei;Qi, Xiaole;Wang, Yongqiang;Gao, Honglei;Gao, Yulong;Wang, Xiaomei;Li, Xiaofei;Qin, Liting;Sun, Yingjun;Cui, Xuezhi;Gao, Yadong;Zhu, Haibo

作者机构：

关键词： avian leukosis virus;P27 protein;monoclonal antibodies;epitope

期刊名称： ARCHIVES OF VIROLOGY

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年卷期：

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收录情况： SCI

摘要： Avian leukosis virus (ALV) is an avian oncogenic retrovirus that can induce various clinical tumors. The capsid protein P27 is the group-specific antigen of ALV and has many viral antigen sites that are easy to detect. In this study, we produced a monoclonal antibody (mAb), 3A9, that is specific for the P27 protein. A series of partially overlapping peptides were screened to define (181)PPSAR(185) as the minimal linear epitope recognized by mAb 3A9. The identified epitope could be recognized by chicken anti-ALV and mouse anti-ALV P27 sera. The epitope was highly conserved among a number of ALV-A, ALV-B and ALV-J strains. MAb 3A9 might be a valuable tool for the development of new immunodiagnostic approaches for ALV, and the defined linear epitope might help further our understanding of the antigenic structure of the P27 protein.

分类号： R37

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