数字农科院2.0

Bio-safety evaluation of Cry1Ac, Cry2Ab, Cry1Ca, Cry1F and Vip3Aa on Harmonia axyridis larvae

文献类型： 外文期刊

作者： Ali, I.;Zhang, S.;Cui, J. -J.;Ali, I.

作者机构：

关键词： Bt proteins;environmental risk assessment;genetically engineered crops;non-target species

期刊名称： JOURNAL OF APPLIED ENTOMOLOGY

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Dietary exposure studies are initial steps in environmental risk assessments of genetically engineered plants on non-target organisms. These studies are conducted in the laboratory where surrogate species are exposed to purified and biologically active insecticidal compounds at higher concentrations than those expected to occur in transgenic crops foliage. Thus, dietary exposure (early tier) tests provide robust data needed to make general conclusions about the susceptibility of the surrogate species to the test substance. For this, we developed suitable artificial diet and used it to establish a dietary exposure test for assessing the toxicity of midgut-active insecticidal compounds to the larvae of the Asian ladybird beetle Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae). Using boric acid as a model compound, we validated the bioassay established for H.axyridis larvae. An artificial diet containing boric acid which negatively affected survival, development and adult weights was offered to larvae and indicated that the bioassay was able to detect toxic effects of insecticidal substances incorporated in diets. Using this dietary exposure test, environmental risk assessment of Cry1Ac, Cry2Ab, Cry1Ca, Cry1F and the non-Cry protein Vip3Aa was evaluated by analysing pupation rates, adult emergence rates, 7-day larval weights, and freshly emerged male and female weights among the toxin treatments and a pure artificial diet. These life-table parameters did not vary among artificial diets containing 200g/g Bt proteins or pure artificial diet. In contrast, boric acid adversely affected all life-table parameters. Thus on these bases, we concluded H.axyridis larvae are not sensitive to these Bt proteins expressed in genetically engineered crops.

分类号： Q969.9

  • 相关文献

[1]Bio-Safety Evaluation Of Cry1Ac, Cry2Ab, C.ry1Ca, Cry1F And Vip3Aa O n Harmonia Axyridis Larvae. Cui, J. -J.,Zhang, S.,Ali, I.,Ali, I.. 2017

[2]Neutral Dietary Effects of Two MicroRNAs, Csu-Novel-260 and Csu-Mir-14, on the Non-Target Arthropod Folsomia candida. Qinli Zhou,Lanzhi Han,Yunhe Li,Jing Li,Xiaowei Yang. 2023

[3]Consumption of Bt rice pollen expressing Cry2Aa does not cause adverse effects on adult Chrysoperla sinica Tjeder (Neuroptera: Chrysopidae). Wang, Yuanyuan,Li, Yunhe,Chen, Xiuping,Zhang, Jie,Chen, Hongyin,Peng, Yufa,Romeis, Joerg. 2012

[4]Tier-1 assays for assessing the toxicity of insecticidal proteins produced by genetically engineered plants to non-target arthropods. Romeis, Joerg,Wu, Kong-Ming,Peng, Yu-Fa,Romeis, Joerg. 2014

[5]Molecular-based environmental risk assessment of three varieties of genetically engineered cows. Xu, Jianxiang,Zhao, Jie,Wang, Jianwu,Zhao, Yaofeng,Zhang, Lei,Li, Ning,Chu, Mingxing. 2011

[6]Toxicological, Biochemical, and Histopathological Analyses Demonstrating That Cry1C and Cry2A Are Not Toxic to Larvae of the Honeybee, Apis mellifera. Li, Yun-He,Chen, Xiu-Ping,Romeis, Joerg,Peng, Yu-Fa,Huang, Zachary Y.,Romeis, Joerg,Dai, Ping-Li.

[7]Adsorption and degradation of triazophos, chlorpyrifos and their main hydrolytic metabolites in paddy soil from Chaohu Lake, China. Liang, Bin,Yang, Chengli,Zhao, Yanfu,Zhang, Jun,Jiang, Jiandong,Li, Shunpeng,Gong, Mingbo,Zhu, Changxiong. 2011

[8]Biosafety management and commercial use of genetically modified crops in China. Peng, Yufa,Wu, Kongming,Hallerman, Eric M.. 2014

[9]Use of an artificial diet system to study the toxicity of gut-active insecticidal compounds on larvae of the green lacewing Chrysoperla sinica. Hu, Long,Romeis, Joerg,Wang, Yanan,Han, Lanzhi,Chen, Xiuping,Peng, Yufa,Romeis, Joerg. 2014

[10]Pollen from Cry1Ac/CpTI-transgenic cotton does not affect the pollinating beetle Haptoncus luteolus. Lizhen Chen,Jinjie Cui,Weihua Ma,Changying Niu,Chaoliang Lei. 2011

[11]Bt RICE PRODUCING Cry1C PROTEIN DOES NOT HAVE DIRECT DETRIMENTAL EFFECTS ON THE GREEN LACEWING CHRYSOPERLA SINICA ( TJEDER). Chen, Xiuping,Hu, Long,Romeis, Joerg,Peng, Yufa,Romeis, Joerg. 2014

[12]Development of a Tier-1 Assay for Assessing the Toxicity of Insecticidal Substances Against Coleomegilla maculata. Chen, Mao,Liu, Xiaoxia,Shelton, Anthony M.,Ostrem, Jared,Romeis, Joerg,Hellmich, Richard L.,Hellmich, Richard L.,Li, Yunhe,Peng, Yufa.

[13]A laboratory assessment of the potential effect of Cry1Ab/Cry2Aj-containing Bt maize pollen on Folsomia candida by toxicological and biochemical analyses. Zhou, Xiang,Zhang, Bing,Yang, Yan,Peng, Yufa,Li, Yunhe,Shen, Ping.

[14]Bt Rice In China - F.ocusing The Nontarget Risk A ssessment. Li, YH,Zhang, QL,Liu, QS,Meissle, M,Yang, Y,Wang, YN,Hua, HX,Chen, XP,Peng, YF,Romeis, J. 2017

[15]A Multi-Generational Risk Assessment Of C.ry1F On The Non-Target S oil Organism Folsomia Candida (Collembola) Based On Whole Transcriptome Profiling. Chen, Wan-Jun,Luan, Yun-Xia,Luan, Yun-Xia,Li, Yunhe,Huang, Cheng-Wang,Ke, Xin. 2019

[16]Do tillage systems affect the cadmium threshold in farmland soil for environmental quality standard setting?. Zhaolin Du,Weiqiang Dou,Dasong Lin,Li Qin,Yi An,Hongan Chen,Lina Wu,Liyan Mou. 2023

[17]A nano-pesticide with low toxicity for earthworms obtained by immobilizing avermectin onto Mg/Al layered double hydroxides. Linlin Shi,Fengshou Dong,Jun Xu,Xiaohu Wu,Yongquan Zheng,Xinglu Pan. 2025

作者其他论文 更多>>