数字农科院2.0

The microRNA expression profile in porcine skeletal muscle is changed by constant heat stress

文献类型： 外文期刊

作者： Hao, Y.;Liu, J. R.;Yang, P. G.;Feng, Y. J.;Cui, Y. J.;Yang, C. H.;Gu, X. H.;Liu, J. R.;Zhang, Y.

作者机构：

关键词： heat shock;longissimus dorsi muscle;miRNA;pig

期刊名称： ANIMAL GENETICS

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Heat stress has profound effects on animal performance and muscle function, and microRNAs (miRNAs) play a critical role in muscle development and stress responses. To characterize the changes in miRNAs in skeletal muscle responding to heat stress, the miRNA expression profiles of longissimus dorsi muscles of pigs raised under constant heat stress (30 degrees C; n=8) or control temperature (22 degrees C; n=8) for 21days were analyzed by Illumina deep sequencing. A total of 58 differentially expressed miRNAs were identified with 30 down-regulated and 28 up-regulated, and 63 differentially expressed target genes were predicted by miRNA-mRNA joint analysis. GO and KEGG analyses showed that the genes regulated by differentially expressed miRNAs were enriched in glucose metabolism, cytoskeletal structure and function and stress response. Real-time PCR showed that the mRNA levels of PDK4, HSP90 and DES were significantly increased, whereas those of SCD and LDHA significantly decreased by heat exposure. The protein levels of CALM1, DES and HIF1 were also significantly increased by constant heat. These results demonstrated that the change in miRNA expression in porcine longissimus dorsi muscle underlies the changes in muscle structure and metabolism in porcine skeletal muscle affected by constant heat stress.

分类号： Q953

  • 相关文献

[1]Differential gene expression drives muscle metabolic and structural differences in Liang Guang small spotted vs. large white pigs. Zheng Feng,Wen Jing Wang,Nan Wang,Shun Shun Chi,Yu Lian Mu,Zhi Guo Liu. 2025

[2]Repertoire of porcine MicroRNAs in adult ovary and testis by deep sequencing. Chen, Haosi,Wang, Xin,Gao, Xiaolian,Li, Mingzhou,Liu, Yingkai,Wang, Tao,Guan, Jiuqiang,Luo, Zonggang,Ma, Jideng,Mu, Zhiping,Jiang, An-an,Zhu, Li,Li, Xuewei,Chen, Lei,Wang, Jinyong,Lang, Qiulei,Zhou, Xiaochuan,Zeng, Wenxian,Li, Ning,Li, Kui.

[3]PPAR gamma is regulated by miR-27b-3p negatively and plays an important role in porcine oocyte maturation. Song, Chunlei,Liang, Xiaojuan,Tao, Cong,Li, Kui,Wang, Yanfang,Yao, Jing,Cao, Chunwei,Huang, Jiaojiao,Han, Ziqiang,Zhang, Yang,Qin, Guosong,Zhao, Jianguo,Yao, Jing,Cao, Chunwei,Huang, Jiaojiao,Zhang, Yang,Qin, Guosong,Zhao, Jianguo,Han, Ziqiang,Li, Chengbo,Yang, Haoran.

[4]Identification of potential miRNA-mRNA regulatory network and the key miRNAs in intramuscular and subcutaneous adipose. Feng, Hui,Liu, Tianyi,Yousuf, Salsabeel,Zhang, Xiuxiu,Huang, Wanlong,Li, Ai,Xie, Lingli,Miao, Xiangyang. 2022

[5]Integrative Analysis of Proteomics and Transcriptomics of Longissimus dorsi with Different Feeding Systems in Yaks. Ma X.,Guo X.,La Y.,Wu X.,Chu M.,Bao P.,Yan P.,Liang C.. 2023

[6]Incorporating genome-wide and transcriptome-wide association studies to identify genetic elements of longissimus dorsi muscle in Huaxi cattle. Mang Liang,Bingxing An,Tianyu Deng,Lili Du,Keanning Li,Sheng Cao,Yueying Du,Lingyang Xu,Lupei Zhang,Xue Gao,Yang Cao,Yuming Zhao,Junya Li,Huijiang Gao. 2023

[7]A comprehensive study on the longissius dorsi muscle of Ashdan yaks under different feeding regimes based on transcriptomic and metabolomic analyses. Wang, Tong,Ma, Xiaoming,Zheng, Qingbo,Ma, Chaofan,Zhang, Zhilong,Pan, Heping,Guo, Xian,Wu, Xiaoyun,Chu, Min,Liang, Chunnian,Yan, Ping. 2024

[8]SHORT COMMUNICATION: Climate change and biofuel wheat: A case study of southern Saskatchewan. Wang, Hong,He, Yong,McConkey, Brian,Cutforth, Herb,McCaig, Tom,McLeod, Grant,Zentner, Robert,DePauw, Ron,Brandt, Kelsey,Liu, Tingting,He, Yong,Qian, Budong,Lemke, Reynald,Liu, Tingting,Qin, Xiaobo,White, Jeffrey,Hunt, Tony,Hoogenboom, Gerrit. 2012

[9]Effect of heat shock on the susceptibility of Frankliniella occidentalis (Thysanoptera: Thripidae) to insecticides. Zuo Tai-qiang,Sun Li-juan,Wang Si-fang,Zheng Chang-ying,Wan Fang-hao,Wan Fang-hao,Li Hong-gang. 2016

[10]Differential gene expression in whitefly (Bemisia tabaci) B-biotype females and males under heat-shock condition. Wan, Fang-Hao,Wan, Fang-Hao.

[11]Enhancement of transglutaminase production in Streptomyces mobaraensis DSM 40587 by non-nutritional stress conditions: Effects of heat shock, alcohols, and salt treatments. Zhang, Lili,Zhang, Lanwei,Yi, Huaxi,Du, Ming,Zhang, Yingchun,Han, Xue,Li, Jingyan,Jiao, Yuehua,Guo, Chunfeng,Feng, Zhen,Zhang, Yanhe.

[12]Gene expression controlled by heat-inducible site-specific. recombination in tobacco. Chen, M,Wang, LX,Peng, XL,Xu, HJ,Lin, ZP. 2003

[13]Water deficits and heat shock effects on photosynthesis of a transgenic Arabidopsis thaliana constitutively expressing ABP9, a bZIP transcription factor. Zhang, Xia,Zhao, Jun,Zhang, Xia,Wollenweber, Bernd,Jiang, Dong,Jiang, Dong,Liu, Fulai.

[14]Effects of Heat Shock on the Bradysia odoriphaga (Diptera: Sciaridae). Cheng, Jiaxu,Su, Qi,Shi, Caihua,Yang, Yuting,Cheng, Jiaxu,Han, Haolin,Xie, Wen,Guo, Zhaojiang,Wu, Qingjun,Xu, Baoyun,Wang, Shaoli,Zhang, Youjun,Jiao, Xiaoguo.

[15]Characteristic analysis of heat-shock-cognate 70-4 gene promoter from silkworm, Bombyx mori (Bmhsc70-4). Tang, Shun-ming,Qui, Zhiyong,Zhao, Qiao-ling,Yi, Yong-zhu,Zhang, Zhi-fang. 2011

[16]Impact of heat waves on egg survival and biological performance across life stages in the Asian corn borer. Quan Y.,Mason C.E.,He K.,Wang Z.,Wei H.. 2023

[17]HY5-HDA9 orchestrates the transcription of HsfA2 to modulate salt stress response in Arabidopsis. Yang, Jiaheng,Qu, Xiao,Li, Tao,Gao, Yixiang,Du, Haonan,Zheng, Lanjie,Ji, Manchun,Zhang, Paifeng,Zhang, Yan,Hu, Jinxin,Liu, Liangyu,Lu, Zefu,Yang, Zijian,Zhang, Huiyong,Yang, Jianping,Jiao, Yongqing,Zheng, Xu. 2022

[18]Combined treatment with IGF1, CoQ10, and melatonin improves the quality of bovine oocytes and heat-shocked blastocysts†. Zhang, Hang,Yang, Baigao,Zhang, Peipei,Cao, Jianhua,Zhang, Xiaomeng,Gahallah, Smhaa,Roth, Zvi,Wan, Pengcheng,Zhao, Xueming. 2025

[19]猪流行性腹泻病毒感染猪小肠上皮细胞miRNA表达谱分析及验证. 张宸语,陈佳宁,温建新,刘光亮. 2018

[20]Bmo-miR-2788对家蚕丝胶蛋白基因Ser-1的表达调控(英文). Rehana Kandhro,陈艳花,钱平,朱娟,沈兴家. 2019

作者其他论文 更多>>