数字农科院2.0

FIP-sch2, a new fungal immunomodulatory protein from Stachybotrys chlorohalonata, suppresses proliferation and migration in lung cancer cells

文献类型： 外文期刊

作者： Li, Shuying;Jiang, Zhonghao;Xu, Wenyi;Xie, Yingying;Zhao, Leiming;Tang, Xuanming;Wang, Fengzhong;Xin, Fengjiao

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关键词： Fungal immunomodulatory protein;Proliferation;Apoptosis;Migration

期刊名称： APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

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收录情况： SCI

摘要： Fungal immunomodulatory protein (FIP)-sch2, an immunomodulatory protein identified in the ascomycete Stachybotrys chlorohalonata by a sequence similarity search, is a novel member of the FIP family. FIP-sch2 shares high sequence identity, structure, and evolutionary conservation with previously reported FIPs. It was satisfactorily expressed in Escherichia coli with a glutathione S-transferase (GST) tag and purified by GST-affinity magnetic beads. To characterize the direct antitumor effects, human lung adenocarcinoma A549 cells were treated with different concentrations of recombinant FIP (rFIP)-sch2 in vitro, and the results showed that rFIP-sch2 could reduce cell viability dose-dependently with a half-maximal inhibitory concentration (IC50) of 9.48 mu g/mL. Furthermore, rFIP-sch2 at 8 mu g/mL could significantly induce apoptosis and interrupt migration in A549 cells. Notably, the antitumor effect of rFIP-sch2 was equivalent to that of rLZ-8 but was obviously increased compared to rFIP-fve. In addition, the exploration of the antitumor mechanism suggested that rFIP-sch2 induced lung cancer cell death by activating apoptosis and inhibiting migration. Our results indicated that rFIP-sch2 was a promising candidate for use in future cancer therapy.

分类号： Q939.9`Q81

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