数字农科院2.0

3-Hydroxyisobutyryl-CoA hydrolase involved in isoleucine catabolism regulates triacylglycerol accumulation in Phaeodactylum tricornutum

文献类型： 外文期刊

作者： Pan, Yufang;Yang, Juan;Li, Xiaolong;Hu, Hanhua;Yang, Juan;Gong, Yangmin

作者机构：

关键词： diatom;propionyl-CoA carboxylase;branched-chain amino acid catabolism;beta-oxidation

期刊名称： PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Since methylmalonyl-CoA epimerase appears to be absent in the majority of photosynthetic organisms, including diatoms, (S)-methylmalonyl-CoA, the intermediate of isoleucine (Ile) catabolism, cannot be metabolized to (R)methylmalonyl-CoA then to succinyl-CoA. In this study, propionyl-CoA carboxylase (PCC) RNAi silenced strains and 3-hydroxyisobutyryl-CoA hydrolase (HIBCH) overexpression strains were constructed to elucidate the Ile degradation pathway and its influence on lipid accumulation in Phaeodactylum tricornutum based on growth, neutral lipid content and metabolite profile analysis. Knockdown of PCC disturbed the metabolism of Ile through propionyl-CoA to methylmalonyl-CoA, as illustrated by much higher Ile content at day 6. However, Ile decreased to comparable levels to the wild-type at day 10. PCC silencing redirected propionyl-CoA to acetyl-CoA via a modified beta-oxidation pathway, and transcript levels for some branched-chain amino acid (BCAA) degradation-related genes, especially HIBCH, significantly upregulated in the PCC mutant, which enhanced the BCAA degradations and thus resulted in higher triacylglycerol (TAG) content. Overexpression of HIBCH accelerates Ile degradation and results in a lowered Ile content in the overexpression strains, thus enhancing carbon skeletons to the tricarboxylic acid cycle and giving rise to increasing TAG accumulation. Our study provides a good strategy to obtain high-lipid-yield transgenic diatoms by modifying the propionyl-CoA metabolism.

分类号： Q

  • 相关数据
  • 相关文献

[1]Supplementary Figures from 3-hydroxyisobutyryl-CoA hydrolase involved in isoleucine catabolism regulates triacylglycerol accumulation in Phaeodactylum tricornutum. Yang, Juan,Hanhua Hu,Yangmin Gong,Yufang Pan,Xiaolong Li

[2]Supplementary Data Sets from 3-hydroxyisobutyryl-CoA hydrolase involved in isoleucine catabolism regulates triacylglycerol accumulation in Phaeodactylum tricornutum. Hanhua Hu,Xiaolong Li,Yang, Juan,Yangmin Gong,Yufang Pan

[3]Supplementary material from "3-hydroxyisobutyryl-CoA hydrolase involved in isoleucine catabolism regulates triacylglycerol accumulation in Phaeodactylum tricornutum". Hanhua Hu,Yufang Pan,Yang, Juan,Yangmin Gong,Xiaolong Li

[1]Tung Tree (Vernicia Fordii, Hemsl.) G.enome And Transcriptome Sequencing R eveals Co-Ordinate Up-Regulation Of Fatty Acid Beta-Oxidation And Triacylglycerol Biosynthesis Pathways During Eleostearic Acid Accumulation In Seeds. Li, Rongjun,Lin, Qiang,Fang, Dongming,Lv, Shiyou,Lv, Shiyou,Hu, Songnian,Shockey, Jay,Cheng, Junyong,Cui, Peng,Gao, Fei,Hu, Songnian,Zhang, Lingling. 2018

[2]3-Hydroxyisobutyryl-Coa Hydrolase Involved In Isoleucine C.atabolism Regulates Triacylglycerol Accumulation I n Phaeodactylum Tricornutum. Pan, YF, Yang, J, Gong, YM, Li, XL, Hu, HH. 2017

[3]Warming and microplastic pollution shape the carbon and nitrogen cycles of algae. Hou X.,Mu L.,Hu X.,Guo S.. 2023

[4]Polystyrene microplastics at environmentally realistic concentrations exacerbate diatom blooms caused by phosphorus pollution: Rethinking coastal eutrophication. Hou, Xuan,Waseem, Muhammad,Boota, Muhammad Waseem,Mu, Li,Wei, Yuanyuan,Hu, Xiangang. 2025

作者其他论文 更多>>