数字农科院2.0

Genome-wide association analysis revealed that GhD6PKL2 regulates cold tolerance at seed germination and seedling emergence in cotton

文献类型： 外文期刊

作者： Shen, Weihe;Li, Shuanglan;Zhou, Binghan;Li, Jinglin;Dong, Yaxin;Liu, Ruihua;Shen, Qian;Liu, Shaodong;Ma, Huijuan;Zhang, Siping;Pang, Chaoyou;Ge, Changwei

作者机构：

关键词： Cotton;Germination and emergence stage;Cold-tolerant traits;GWAS:Gene function verification

期刊名称： ENVIRONMENTAL AND EXPERIMENTAL BOTANY

ISSN： 0098-8472

年卷期： 2025 年 241 卷

页码：

收录情况： SCIE（2025版）

摘要： Low temperature (12 degrees C) is an abiotic stress factor that adversely affects cotton yield and fiber quality. Exposure to low-temperature stress during seed germination and seedling emergence significantly reduces crop productivity. In this study, 383 cotton germplasms were subjected to low-temperature stress, and the variation in seven seedling emergence-related phenotypic traits, including germination rate, was determined. Through genome-wide association analysis (GWAS), we identified 331 significantly associated single nucleotide polymorphism (SNP) loci, which were mapped to traits including hypocotyl length, total seedling length, dry weight, and germination rate. Notably, a quantitative trait locus (QTL) associated with hypocotyl length was identified on chromosome A08 (51.91-51.93 Mb). Further analysis revealed that this significant genomic region harbors polymorphic sites within the promoter of GhD6PKL2. Transcriptome sequencing and quantitative real-time PCR (qRT-PCR) further revealed significant differences in this gene's expression levels across distinct haplotypes. Virus-induced gene silencing (VIGS) of GhD6PKL2 demonstrated that TRV2: GhD6PKL2 plants exhibited hypocotyl elongation under low-temperature treatment, accompanied by reduced cotton germination rates,indicating that this gene positively regulates cold resistance in cotton. This study provides valuable theoretical foundations for the breeding and improvement of low-temperature tolerant cotton varieties.

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