数字农科院2.0

Antibody-Dependent Cellular Cytotoxicity Elicited by the Antibodies Against the E120R Protein of African Swine Fever Virus

文献类型： 外文期刊

作者： Shengmei Chen;Jing Lan;Zhanhao Lu;Jia Li;Caoyuan Ma;Rui Luo;Qiang Fu;Yuan Sun;Tao Wang;Hua Ji Qiu

作者机构：

关键词： African swine fever;African swine fever virus;antibodies;antibody-dependent cellular cytotoxicity;E120R protein

期刊名称： Vaccines

ISSN： 2076-393X

年卷期： 2025 年 13 卷 9 期

页码：

收录情况： SCIE（2025版）

摘要： Background/Objectives: African swine fever (ASF) is a disease of domestic pigs and wild boar caused by African swine fever virus (ASFV), in which infection often leads to high morbidity and mortality. Although subunit and mRNA vaccines based on protective antigens have been explored for ASFV, their protective efficacy remains insufficient for practical ASF control, highlighting the need to identify new potential antigens capable of inducing more potent and broadly protective immune responses. Previously, we found that the antibodies against the ASFV E120R protein (pE120R) could significantly inhibit virus replication in primary porcine alveolar macrophages (PAMs). However, it is not yet known whether anti-pE120R antibodies can induce antibody-dependent cellular cytotoxicity (ADCC). Methods: In this study, we analyzed the conservation and immunogenic features of pE120R and established an HEK293T cell line with stable expression of pE120R as target cells (HEK293T-pE120R). Additionally, a co-culture system comprising target cells and peripheral blood mononuclear cells (PBMCs) was established to evaluate the ability of the anti-pE120R antibodies to induce ADCC as measured by lactate dehydrogenase (LDH) release assays. Results: The results showed that pE120R is highly conserved among different ASFV genotypes and contains multiple B-cell and T-cell epitopes. Importantly, LDH release assays demonstrated that anti-pE120R antibodies triggered NK cell-mediated ADCC. Notably, ASFV replication in HEK293T-pE120R cells was not promoted. Conclusions: In summary, pE120R was associated with antibody production in a cytotoxicity assay. The ability of this antigen to induce protective immunity, if any, requires further evaluation in vivo.

分类号：

  • 相关文献

[1]An immunoassay based on bioluminescent sensors for rapid detection of African swine fever virus antibodies. Zhang, Zhonghui,Wang, Jinming,Niu, Qingli,Guan, Guiquan,Yin, Hong,Yang, Jifei. 2024

[2]Development of a Ready-To-Use Bioluminescence Immunosensor for the One-Step Sensitive Detection of Antibodies Against African Swine Fever Virus. Zhonghui Zhang,Xuesai Li,Qingli Niu,Jinming Wang,Yanghe Liu,Dossêh Jean Apôtre Afayibo,Wenting Chen,Songlin Yang,Hong Yin,Guiquan Guan,Jifei Yang. 2025

[3]Structure and function of African swine fever virus proteins: Current understanding. Sicheng Yang,Chun Miao,Wei Liu,Guanglei Zhang,Junjun Shao,Huiyun Chang. 2023

[4]Mammalian Cell-Line-Expressed CD2v Protein of African Swine Fever Virus Provides Partial Protection against the HLJ/18 Strain in the Early Infection Stage. Rong Hong Hua,Jing Liu,Shu Jian Zhang,Ren Qiang Liu,Xian Feng Zhang,Xi Jun He,Dong Ming Zhao,Zhi Gao Bu. 2023

[5]Genetic Variations of African Swine Fever Virus: Major Challenges and Prospects. Shengmei Chen,Tao Wang,Rui Luo,Zhanhao Lu,Jing Lan,Yuan Sun,Qiang Fu,Hua Ji Qiu. 2024

[6]A novel high-throughput screen identifies phenazine-1-carboxylic acid as an inhibitor of African swine fever virus replication in primary porcine alveolar macrophages. Lan, Jing,Luo, Rui,Liu, Di,Qi, Changxing,Song, Xin,Lu, Zhanhao,Huang, Ruojia,Yang, Yuying,Sun, Yuan,Zhang, Yonghui,Wang, Tao,Qiu, Hua-Ji. 2025

[7]A Standardized Pipeline for Assembly and Annotation of African Swine Fever Virus Genome. Edward Spinard,Mark Dinhobl,Cassidy N.G. Erdelyan,James O’Dwyer,Jacob Fenster,Hillary Birtley,Nicolas Tesler,Sten Calvelage,Mikael Leijon,Lucilla Steinaa,Vivian O’Donnell,Sandra Blome,Armanda Bastos,Elizabeth Ramirez-Medina,Anna Lacasta,Karl Ståhl,Huaji Qiu,Dachrit Nilubol,Chandana Tennakoon,Charles Maesembe,Bonto Faburay,Aruna Ambagala,David Williams,Paolo Ribeca,Manuel V. Borca,Douglas P. Gladue. 2024

[8]A Pool of Ferritin Nanoparticles Delivering Six Proteins of African Swine Fever Virus Induces Robust Humoral and Cellular Immune Responses in Pigs. Lu, Zhanhao,Zhong, Dailang,Song, Xin,Lan, Jing,Wang, Yanjin,Luo, Rui,Chen, Shengmei,Huang, Ruojia,Qiu, Hua-Ji,Li, Yongfeng,Wang, Tao,Sun, Yuan. 2026

[9]Expression, purification, and characterization of recombinant NS-1, the porcine parvovirus non-structural protein. Qi, Ting,Cui, Shang-jin.

[10]Synthesis of empty capsid-like particles of Asia I foot-and-mouth disease virus in insect cells and their immunogenicity in guinea pigs. Cao, Yimei,Lu, Zengjun,Sun, Jiachuan,Bai, Xingwen,Sun, Pu,Bao, Huifang,Chen, Yingli,Guo, Jianhong,Li, Dong,Liu, Xiangtao,Liu, Zaixin.

[11]Seroprevalence of Toxoplasma gondii and Neospora caninum in Tarim Red Deer (Cervus elaphus yarkandensis) from Xinjiang Province, Northwest China. Meng, Q. L.,Qiao, J.,Wang, W. S.,Chen, C. F.,Zhang, Z. C.,Cai, K. J.,Tian, Z. Z.,Yang, L. H.,Cai, X. P.,Tian, G. F.. 2012

[12]Immunogenicity and protective efficacy in monkeys of purified inactivated Vero-cell SARS vaccine. Qin, E,Shi, HY,Tang, L,Wang, C,Chang, GH,Ding, ZF,Zhao, K,Wang, J,Chen, Z,Yu, M,Si, BY,Liu, JY,Wu, DL,Cheng, XJ,Yang, BA,Peng, WM,Meng, QW,Liu, BH,Han, WG,Yin, XA,Duan, HY,Zhan, DW,Tian, L,Li, SL,Wu, JS,Tan, G,Li, Y,Li, YC,Liu, YG,Liu, H,Lv, FS,Zhang, Y,Kong, XG,Fan, BC,Jiang, T,Xu, SL,Wang, XM,Li, CW,Wu, XH,Deng, YQ,Zhao, M,Zhu, QY.

[13]Development of blocking ELISA for detection of antibodies against H9N2 avian influenza viruses. Li, Zejun.

[14]Protective efficacy of commercial Newcastle disease vaccines against challenge of goose origin virulent Newcastle disease virus in geese. Dai, Yabin,Liu, Mei,Li, Wenliang.

[15]Field testing of Schistosoma japonicum DNA vaccines in cattle in China. Shi, FH,Zhang, YB,Lin, JJ,Zuo, X,Shen, W,Cai, YM,Ye, P,Bickle, QD,Taylor, MG.

[16]Serological detection of bovine ephemeral fever virus using an indirect ELISA based on antigenic site G(1) expressed in Pichia pastoris. Qiu, Chang-Qing.

[17]Fusion of C3d with hemagglutinin enhances protective immunity against swine influenza virus. Li, Guo-Xin,Tian, Zhi-Jun,Yu, Hai,Jin, Yuan-Yuan,Hou, Shao-Hua,Zhou, Yan-Jun,Liu, Tian-Qiang,Hu, Shou-Ping,Tong, Guang-Zhi,Li, Guo-Xin,Tong, Guang-Zhi,Li, Guo-Xin,Yu, Hai,Zhou, Yan-Jun,Tong, Guang-Zhi.

[18]Laboratory and field evaluation of Schistosoma japonicum DNA vaccines in sheep and water buffalo in China. Shi, FH,Zhang, YB,Ye, P,Lin, JJ,Cai, YM,Shen, W,Bickle, QD,Taylor, MG.

[19]Screening of strain-specific Actinobacillus pleuropneumoniae genes using a combination method. Lei, Liancheng,Wang, Jiaqi,Lei, Liancheng,Du, Chongtiao,Yang, Peng,Xie, Fang,Ou, Pingyang,Han, Wenyu.

[20]Plasmid containing CpG oligodeoxynucleotides can augment the immune responses of pigs immunized with porcine reproductive and respiratory syndrome killed virus vaccine. Quan, Zhang,Qin, Zhai Guo,Zhen, Wang,Feng, Xue Zheng,Hong, Jia,Fei, Zhu Hong.

作者其他论文 更多>>