数字农科院2.0

The effects of Bt Cry1le toxin on bacterial diversity in the midgut of Apis mellifera ligustica (Hymenoptera: Apidae)

文献类型： 外文期刊

作者： Wang, Qiang;Diao, Qing-Yun;Zhou, Ting;Dai, Ping-Li;Jia, Hui-Ru;Geng, Li-Li;Li, Yun-He

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期刊名称： SCIENTIFIC REPORTS

ISSN： 2045-2322

年卷期： 2016 年 6 卷

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收录情况： SCI

摘要： The honey bee has been regarded as a key species in the environmental risk assessment of biotech crops. Here, the potential adverse effects of Cry1Ie toxin on the midgut bacteria of the worker bees (Apis mellifera ligustica) were investigated under laboratory conditions. Newly emerged bees were fed with different concentrations of Cry1Ie toxin syrups (20 ng/mL, 200 ng/mL, and 20 mu g/mL), pure sugar syrup, and 48 ppb of imidacloprid syrups, then sampled after 15 and 30 d. We characterized the dominant midgut bacteria and compared the composition and structure of the midgut bacterial community in all samples using the Illumina MiSeq platform targeting the V3-V4 regions of 16S rDNA. No significant differences in the diversity of the midgut bacteria were observed between the five treatments. This work was the first to show the effects of Cry1Ie toxin on honey bees, and our study provided a theoretical basis for the biosafety assessment of transgenic Cry1Ie maize.

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