数字农科院2.0

Quantitative Detection of the Foot-And-Mouth Disease Virus Serotype 0 146S Antigen for Vaccine Production Using a Double-Antibody Sandwich ELISA and Nonlinear Standard Curves

文献类型： 外文期刊

作者： Feng, Xia;Ma, Jun-Wu;Sun, Shi-Qi;Guo, Hui-Chen;Yang, Ya-Min;Jin, Ye;Zhou, Guang-Qing;He, Ji-Jun;Guo, Jian-Hong;Qi, Shu-yun;Lin, Mi;Cai, Hu;Liu, Xiang-Tao

作者机构：

期刊名称： PLOS ONE

ISSN： 1932-6203

年卷期： 2016 年 11 卷 3 期

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收录情况： SCI

摘要： The efficacy of an inactivated foot-and-mouth disease (FMD) vaccine is mainly dependent on the integrity of the foot-and-mouth disease virus (FMDV) particles. At present, the standard method to quantify the active component, the 146S antigen, of FMD vaccines is sucrose density gradient (SDG) analysis. However, this method is highly operator dependent and difficult to automate. In contrast, the enzyme-linked immunosorbent assay (ELISA) is a time-saving technique that provides greater simplicity and sensitivity. To establish a valid method to detect and quantify the 146S antigen of a serotype 0 FMD vaccine, a double-antibody sandwich (DAS) ELISA was compared with an SDG analysis. The DAS ELISA was highly correlated with the SDG method (R-2 = 0.9215, P<0.01). In contrast to the SDG method, the DAS ELISA was rapid, robust, repeatable and highly sensitive, with a minimum quantification limit of 0.06 mu g/mL. This method can be used to determine the effective antigen yields in inactivated vaccines and thus represents an alternative for assessing the potency of FMD vaccines in vitro. But it still needs to be prospectively validated by analyzing a new vaccine preparation and determining the proper protective dose followed by an in vivo vaccination-challenge study to confirm the ELISA findings.

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