数字农科院2.0

Development of an automatic tubular chemiluminescence immunoassay using magnetic particles for the detection of antibodies against foot-and-mouth disease virus serotype A

文献类型： 外文期刊

作者： Li, Fengsong;Bao, Yanfang;Jiang, Tao;Li, Taoshan;Zuo, Yili;Yang, Guang;Li, Xin;Cui, Yan;Lin, Mi

作者机构：

关键词： Tubular chemiluminescence immunoassay;Automation;Foot-and-mouth disease virus serotype A;Antibody detection;Serum

期刊名称： MICROBIAL PATHOGENESIS

ISSN： 0882-4010

年卷期： 2025 年 199 卷

页码：

收录情况： SCIE（2025版）

摘要： Foot-and-mouth disease virus (FMDV) causes significant financial losses in animal farms worldwide. Specific antibody monitoring of FMDV post-vaccination is important for evaluating vaccine efficacy, as well as the prevention and control of foot-and-mouth disease (FMD). In this study, we developed a fully automatic tubular chemiluminescence immunoassay method based on magnetic particles (A-MPCLIA) to specifically detect antibodies against FMDV serotype A. The diagnostic sensitivity (Dsn) and diagnostic specificity (Dsp) of A-MPCLIA were determined to be 96.05 % and 99 %, respectively, with a cut-off value of 30 activity units (U) by detecting the known background swine, cattle, and sheep serum. The developed method presented good Dsn, Dsp, and repeatability (CV < 10 %). The activity units of A-MPCLIA, determined using 23 animals vaccinated with FMDV serotypes O, A, and Asia 1 univalent inactivated vaccine and nine serum samples from na & iuml;ve animals, were significantly positively correlated with the titers of liquid-phase-blocking enzyme-linked immunosorbent assay (R-2 = 0.992) and the titer of the virus neutralization test (R-2 = 0.9577). In addition, A-MPCLIA requires less than 20 min, and it is fully automatic in detection. These results showed that A-MPCLIA is a suitable method for the detection of antibodies against FMDV serotype A in the future.

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