数字农科院2.0

Characterization and function of Tomato yellow leaf curl virus-derived small RNAs generated in tolerant and susceptible tomato varieties

文献类型: 外文期刊

作者: Bai Miao;Yang Guo-shun;Chen Wen-ting;Lin Run-mao;Ling Jiang;Mao Zhen-chuan;Xie Bing-yang

作者机构:

关键词: Tomato yellow leaf curl virus;virus-induced RNA silencing;virus-derived small RNA;degradome

期刊名称: JOURNAL OF INTEGRATIVE AGRICULTURE

ISSN: 2095-3119

年卷期: 2016 年 15 卷 8 期

页码:

收录情况: SCI

摘要: Virus-tolerant plant, which allows the accumulation of virus and then generates virus-derived small RNAs (vsRNAs), is a valuable material to reveal the antiviral efficiency of vsRNAs. Here, a comparison of vsRNAs in Tomato yellow leaf curl virus tolerant and in susceptible tomato varieties showed the consistent trend of vsRNAs' distribution on virus genome, which is presented as an obvious characteristic. However, the expression level of vsRNA in tolerant variety is less than that in susceptible variety. Slicing targets of vsRNA-mediated viral transcripts were investigated using parallel analysis of RNA ends, and geminivirus DNA methylation was determined by bisulfite sequencing, which uncovered that not all vsRNAs participated in viral mRNA degradation and DNA methylation. Additionally, by comparing with the expression pattern of vsRNAs, viral DNA and mRNA, we proposed the quantity of vsRNAs is corresponding to the expression level of viral mRNA, while the virus-suppression of vsRNAs is not high-efficient.

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