The expression, purification and activity analysis of Francisella tularensis citrulline ureidase in Escherichia coli
文献类型： 外文期刊
作者： Tingheng Zhu *; Sanyu Fang ; Weixia Wang ; Kun Wang ; Zhifeng Cui ; Changchun Wang *
作者机构：
关键词： Citrulline ureidase;Ornithine;Citrulline;Francisella tularensis;Disease diagnosis
期刊名称： Journal of Applied Biomedicine
ISSN： 1214-021X
年卷期： 2015 年 13 卷 3 期
页码：
收录情况： JCR（2021版）
摘要： Citrulline ureidase (CTU, EC3.5.1.20) degrades citrulline into ornithine, carbon dioxide, and ammonia. Here, we present the report on expression of recombinant CTU in Escherichia coli. The soluble and active recombinant CTU was expressed in the periplasmic space with the vector pET-22b and the His-tagged CTU was purified with Ni-Affinity Chromatography. The yield of soluble recombinant protein was significantly increased when 1% sorbitol was supplemented in medium. By using phenylisothiocyanate (PITC) pre-column derivatization HPLC, the enzyme activity of recombinant CTU was determined via measuring of the substrate citrulline and the corresponding products. Our results could be useful in the study of CTU biochemical characteristics, enzymatic preparation of ornithine and development of an enzymatic detection method of citrulline. (C) 2014 Faculty of Health and Social Studies, University of South Bohemia in Ceske Budejovice. Published by Elsevier Sp. z o.o. All rights reserved.
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