数字农科院2.0

Construction of two Gateway vectors for gene expression in fungi

文献类型： 外文期刊

作者： Tingheng Zhu ; Weixia Wang ; Xiao Yang ; Kun Wang ; Zhifeng Cui *

作者机构：

关键词： Fungal expression vector;Gateway cloning technology;Agrobacterium-mediated transformation

期刊名称： Plasmid

ISSN： 0147-619X

年卷期： 2009 年 62 卷 2 期

页码：

收录情况： JCR（2021版）

摘要： We report the construction of two Gateway fungal expression vectors pCBGW and pGWBF. The pCBGW was generated by introducing an expression cassette, which consists of a Gateway recombinant cassette (attR1-Cmr-ccdB-attR2) under the control of fungal promoter PgpdA and a terminator TtrpC, into the multiple cloning site of fungal vector pCB1004. The pGWBF is a binary vector, which was generated from the plant expression vector pGWB2 by replacing the CaMV35S promoter with PgpdA, The pGWBF can be transformed into fungi efficiently with Agrobacterium-mediated transformation. The applicability of two newly constructed vectors was tested by generating the destination vectors pGWBF-GFP and pCBGW-GFP and examining the expression of GFP gene in Trichoderma viride and Gibberella fujikuroi, respectively. Combining with the advantage of Gateway cloning technology, pCBGW and pGWBF will be useful in fungi for large-scale investigation of gene functions by constructing the interested gene destination/expression vectors in a high-throughput way. (c) 2009 Elsevier Inc. All rights reserved.

分类号：

  • 相关文献

[1]Construction of gateway-compatible yeast two-hybrid vectors for high throughput analysis of protein interaction. Tingheng Zhu , Weixia Wang *, Hann-lin Wong , Xiao Yang , Kun Wang , Zhifeng Cui *. 2010

[2]Expressing A Modified Cowpea Trypsin I.nhibitor Gene To Increase I nsect Tolerance Against Pieris Rapae In Chinese Cabbage. Pei, Yanxi,Yang, Guangdong,Zhu, Zhen,Li, Yane,Ma, Xiaoli,Ma, Xiaoli. 2017

[3]Efficient Agrobacterium-mediated transformation of rice by phosphomannose isomerase/mannose selection. Ding Zai-Song,Zhao Ming,Jing Yu-Xiang,Li Liang-Bi,Kuang Ting-Nin. 2006

[4]Approaches to improve the transgenic efficiency and to rescue seedlings from hyperhydricity for rapeseed (Brassica napus). Ding, Yongqiang,Zhang, Dingyu,Yin, Guoying,Wang, Wenjing,Ding, Yongqiang,Zhang, Dingyu,Yin, Guoying,Wang, Wenjing.

[5]Generation of Marker- and/or Backbone-Free Transgenic Wheat Plants via Agrobacterium-Mediated Transformation. Wang, Gen-Ping,Yu, Xiu-Dao,Sun, Yong-Wei,Xia, Lan-Qin,Wang, Gen-Ping,Jones, Huw D.. 2016

[6]Agrobacterium-mediated genetic transformation of peanut and the efficient recovery of transgenic plants. Chen, Mingna,Yang, Qingli,Wang, Tong,Chen, Na,Pan, Lijuan,Chi, Xiaoyuan,Yang, Zhen,Wang, Mian,Yu, Shanlin,Chi, Xiaoyuan. 2015

[7]Studies on Agrobacterium-mediated genetic transformation of embryogenic suspension cultures of sweet potato. Xing, Yu-Jun,Ji, Qin,Luo, Yu-Ming,Xing, Yu-Jun,Yang, Qing,Li, Qiang,Wang, Xin. 2008

[8]Screening Chinese soybean genotypes for Agrobacterium-mediated genetic transformation suitability. Song, Zhang-yue,Tian, Jing-luan,Fu, Wei-zhe,Li, Lin,Lu, Ling-hong,Zhou, Lian,Shou, Hui-xia,Shan, Zhi-hui,Tang, Gui-xiang. 2013

[9]Studies on transfer of Bt gene into Glycine max. Su, YH,Wang, HL,Yu, MM,Lu, DY,Guo, SD. 1999

[10]High-efficiency Agrobacterium-mediated transformation of Lotus corniculatus L. using phosphomannose isomerase positive selection. Guo, Qianqian,Ma, Jiangtao,Yuan, Bei,Wu, Yanmin,Guo, Qianqian,Ma, Jiangtao,Zhou, Meiliang.

[11]Approaches To Improve The Transgenic E.fficiency And To Rescue S eedlings From Hyperhydricity For Rapeseed (Brassica Napus). Ding, YQ, Zhang, DY, Yin, GY, Wang, WJ. 2017

[12]A 4 bp InDel in the Promoter of Wheat Gene TaAFP-B Affecting Seed Dormancy Confirmed in Transgenic Rice. Feng Y.,Han Y.,Han B.,Zhao Y.,Yang Y.,Xing Y.. 2022

[13]An efficient soybean transformation protocol for use with elite lines. Wang, Ying,Li, Zhan,Chen, Xiaorui,Gu, Yongzhe,Zhang, Lijuan,Qiu, Lijuan. 2023

[14]Establishment of an Agrobacterium‐mediated genetic transformation and CRISPR/Cas9‐mediated targeted mutagenesis in Hemp (Cannabis Sativa L.). Xiaoyu Zhang,Gencheng Xu,Chaohua Cheng,Lei Lei,Jian Sun,Ying Xu,Canhui Deng,Zhigang Dai,Zemao Yang,Xiaojun Chen,Chan Liu,Qing Tang,Jianguang Su. 2021

[15]Construction And Genetic Analysis Of A.nthocyanin-Deficient Mutants Induced By T -Dna Insertion In 'Tsuda' Turnip (Brassica Rapa). Wang, XZ, Wang, Y, Chen, BW, Kawabata, S, Fang, ZY, Li, YH. 2017

[16]Mannose selection system used for cucumber transformation. Zhengquan He , ZhenZhen Duan , Wei Liang , Faju Chen , Wei Yao , Hongwei Liang , Chaoyin Yue , Zongxiu Sun , Fan Chen , Jianwu Dai *. 2006

[17]Efficient Induction Of Haploid Plants In Wheat By Editing Of Tamtl Using An Optimized Agrobacterium-Mediated Crispr System. Liu, HY, Wang, K, Jia, ZM, Gong, Q, Lin, ZS, Du, LP, Pei, XW, Ye, XG. 2020

[18]Establishment of an Agrobacterium-mediated genetic transformation and CRISPR/Cas9-mediated targeted mutagenesis in Hemp (Cannabis Sativa L.). Xiaoyu Zhang,Gencheng Xu,Chaohua Cheng,Lei Lei,Jian Sun,Ying Xu,Canhui Deng,Zhigang Dai,Zemao Yang,Xiaojun Chen,Chan Liu,Qing Tang,Jianguang Su. 2021

[19]Identification, charectrization and genetic transformation of lignin and pectin polysaccharides through CRISPR/Cas9 in Nicotiana tobacum. Rana Imtiaz Ahmed,Angyan Ren,Dalal Sulaiman Alshaya,Sajid Fiaz,Yingzhen Kong,Shoaib Liaqat,Naushad Ali,Muhammad Abu Bakar Saddique,Kotb A. Attia,Mahmood Ul Hassan Taga. 2024

[20]Advances in and Perspectives on Transgenic Technology and CRISPR-Cas9 Gene Editing in Broccoli. Li Zhang,Sufang Meng,Yumei Liu,Fengqing Han,Tiemin Xu,Zhiwei Zhao,Zhansheng Li. 2024

作者其他论文 更多>>