数字农科院2.0

A Visual Gel-Enzyme-Linked Immunosorbent Assay for Simultaneous Detection of Gentamicin and Kanamycin in Milk

文献类型： 外文期刊

作者： Xu Fei;Liu Mao-Lin;Liu Yi-Ming;Li Xiu-Bo;Li Jing-Ya;Zhou Jie;Ding Shuang-Yang;Wang Jian-Fen

作者机构：

关键词： Gel-enzyme-linked immunosorbent assay;Gentamicin;Kanamycin;Simultaneous detection;Milk

期刊名称： CHINESE JOURNAL OF ANALYTICAL CHEMISTRY

ISSN： 0253-3820

年卷期： 2015 年 43 卷 6 期

页码：

收录情况： SCI

摘要： A visual gel-enzyme-linked immunosorbent assay (Gel-ELISA) assay for simultaneous detection of gentamicin (GEN) and kanamycin (KANA) in milk was described. In the gel detection column, two detection layers were filled with CN-Br activated Sephrose 4B gel as solid phase carrier, which was coupled with goat anti-mouse IgG. The anti-GEN and anti-KANA monoclonal antibodies were added to combine with the carrier respectively in each layer. Gentamicin and kanamycin drugs in sample competed with corresponding enzyme-labeled antigens for the immobilized antibody on the column, and the drugs could be detected by the chromogenic reaction of substrate 3,3' ,5, 5' -tetramethylbenzidine. This approach was a yes/no method after color detection by naked eyes with the detection time of 15 min. The sensitivity of the Gel-ELISA method was 2 mu g/L, and the detection limit (Cut-off value) for gentamicin and kanamycin in raw milk was 5 mu g/L. The detection results of the ten blind milk samples by the proposed Gel-ELISA method were consistent with those obtained by ultra performance liquid chromatography. tandem mass spectrometry (UPLC/MS-MS).

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