Isolation, molecular characterization and evaluation of the pathogenicity of a porcine rotavirus isolated from Jiangsu Province, China
文献类型： 外文期刊
作者： Zhang, Hewei;Wu, Hua;Zhang, Hewei;Zhang, Zhuo;Wang, Yufei;Wang, Xin;Xia, Mingqi;Wu, Hua;Zhang, Zhuo;Wang, Yufei
作者机构：
期刊名称： ARCHIVES OF VIROLOGY
ISSN： 0304-8608
年卷期： 2015 年 160 卷 5 期
页码：
收录情况： SCI
摘要： In this study, a porcine rotavirus was isolated from a fecal sample from a diarrheic piglet in Jiangsu Province, China. Rotavirus-specific cytopathic effects were observed after 12 blind passages on MA-104 cells, yielding a virus titer of 10(6.125) TCID50/ml. By applying an 80 % nucleotide cutoff value and the RotaC(2.0) automated genotyping tool, the Vp4 genotype of the new isolate was identified as P[7]. The Vp7 genotype was identified as G[9], lineage VI, and sublineage c. Experimentally infected piglets showed severe diarrhea symptoms 16-24 h post-inoculation, indicating that this new porcine rotavirus isolate is a pathogenic strain.
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