数字农科院2.0

Single nucleotide polymorphism screening, molecular characterization, and evolutionary aspects of chicken Piwi genes

文献类型： 外文期刊

作者： Wang, H. Z.;Ma, T.;Chang, G. B.;Wan, F.;Lu, L.;Xu, L.;Chen, J.;Chen, G. H.;Liu, X. P.

作者机构：

关键词： Chicken;Target-capture sequencing;Piwi genes;SNP

期刊名称： GENETICS AND MOLECULAR RESEARCH

ISSN： 1676-5680

年卷期： 2015 年 14 卷 4 期

页码：

收录情况： SCI

摘要： The P-element-induced wimpy testis (Piwi) gene is involved in germline stem cell self-renewal, meiosis, RNA silencing, and transcriptional regulation. Piwi genes are relatively well conserved in many species, but their function in poultry species is unclear. In this study, Piwi genes were sequenced using a target-sequence capture assay in quail and 28 breeds of chicken. Single nucleotide polymorphisms (SNPs) and evolutionary aspects of these chicken breeds were then analyzed. We found that SNP sites existed mainly in the introns of a few chicken breeds, and we selected an SNP on intron 4 for further verification by Sanger sequencing, the results of which were similar to those obtained by the target-capture sequencing assay. The evolutionary analysis revealed that there were more mutations in the Chahua and Leghorn breeds than in the other breeds, and that the phylogenetic tree was divided into four main categories that suggested that Piwi is evolutionarily conserved, and mutations in the introns might be associated with gametogenesis. The screened SNPs can be used as candidate markers for Piwi, and our results provide basic information for the further study of Piwi function in poultry.

分类号：

  • 相关文献

[1]Correlation Analysis Between Single Nucleotide Polymorphisms in Exon 2 of ADSL Gene and Inosine Monophosphate Acid Content in Chicken. Chen, G. H.,Wu, X. S.,Bao, W. B.,Shu, J. T.,Xu, Q.,Zhang, X. Y.,Han, W.. 2009

[2]Correlation analysis between single nucleotide polymorphisms in ADSL gene and IMP content in chicken. Bao, W. B.,Shu, J. T.,Musa, H. H.,Chen, G. H.. 2009

[3]Chicken Polymorphism at Pre-MicroRNAs Inferred from SNP Data. Zhang, Chuan-sheng,Geng, Li-ying,Zhang, Jin,Zhu, Wen-jin,Du, Li-xin. 2010

[4]Polymorphism in NPY and IGF-I genes associate with reproductive traits in Wenchang chicken. Li, H. F.,Zhu, W. Q.,Chen, K. W.,Tang, Q. P.,Gao, Y. S.,Song, W. T.,Xu, H. L.,Wu, X.,Xu, H. L.. 2009

[5]Correlation analysis between single nucleotide polymorphism of interleukin-1 beta gene and interleukin-1 concentration in three chicken breeds. Liu Xiangping,Wang Cunbo,Chang Guobin,Wang Kehua,Chen Guohong,Dou Taocun,Qu Liang,Ma Teng,Chen Rong. 2013

[6]噬菌体展示技术分析鸡贫血病毒VP1蛋白抗原结构特征. 王晓艳,高玉龙,高宏雷,付朝阳,邓小芸,林欢,祁小乐,王笑梅. 2007

[7]GlobalgeneexpressionprofileofchickenspleentissueatalaterstageofIBVinfectionrevealspathogenesisandimmunemechanisms. FengCong,ZongxiHan,FengjieWang,YuhaoShao,XiangangKong,ShengwangLiu. 2014

[8]EIAV基因转移载体启动子的比较. 韩凌霞,李亚明,曲连东,司昌德,姜骞,刘家森. 2006

[9]绵羊Y染色体基因鉴定及其与父系起源的研究进展. 谢云龙,秦芳,李万宏,李发弟,乐祥鹏. 2017

[10]高邮鸭IGF-1基因SNP位点变异与骨骼肌生长发育相关性分析. 陶志云,朱春红,宋卫涛,徐文娟,刘宏祥,章双杰,李慧芳. 2016

[11]猪EPOR基因结构、遗传变异及连锁不平衡分析. 张龙超,王立贤,赵克斌,颜华. 2009

[12]牛SCD1基因C878T位点遗传多态性与中国西门塔尔牛部分脂肪相关性状的效应分析. 武秀香,施雪奎,常玲玲,杨章平,李俊雅,毛永江,高会江. 2011

[13]利用SNP标记估计西门塔尔牛亲缘关系系数的准确性. 张静静,高会江,吴洋,朱波,齐欣,高雪,张路培,陈燕. 2016

[14]NPY、IGF-1基因对文昌鸡繁殖性状的遗传效应. 朱文奇,李慧芳,吴旭,钱凯,张晶鑫,葛洪伟. 2007

[15]神经肽(NPY)基因多态性与邵伯鸡母系繁殖性状的关联性分析. 黄华云,黎寿丰,赵振华,丁余荣,张静. 2010

[16]鸡脂肪酸脱氢酶基因多态性及其与脂肪酸含量的关联分析. 刘向萍,雷黎立,周琼,王克华,常国斌. 2010

[17]奶牛pIgR基因多态性及其对牛奶中IgA和IgM含量的影响. 张春刚,刘光磊,程金波,董晓丽,赵国琦,刘开朗,王加启,卜登攀. 2008

[18]西门塔尔牛饱和脂肪酸含量的低密度芯片基因组预测. 齐欣,张静静,樊惠中,李娟,胡鑫,刘飞,朱波,高雪,陈燕. 2016

[19]紫茎泽兰低温应答转录因子EaCBF1的克隆及不同地理种群的遗传差异分析. 程红梅,郭建英,万方浩. 2007

[20]基于茶树SNP的dCAPS标记体系研究. 张成才,王丽鸳,韦康,成浩,包云秀,刘本英,汪云刚. 2012

作者其他论文 更多>>