数字农科院2.0

Construction of a full-length cDNA library and preliminary analysis of expressed sequence tags from lymphocytes of half-pipe snowboarding athletes

文献类型： 外文期刊

作者： Zhao, Y. H.;Zhang, Z. B.;Zhao, C. Q.;Zhang, Y.;Wang, Y. F.;Zhu, Z. Q.;Guan, W. J.

作者机构：

关键词： Half-pipe snowboarding;Full-length cDNA library;Lymphocyte;Expressed sequence tags

期刊名称： GENETICS AND MOLECULAR RESEARCH

ISSN： 1676-5680

年卷期： 2015 年 14 卷 4 期

页码：

收录情况： SCI

摘要： The genes of top athletes are a valuable genetic resource for the human race, and could be exploited to identify novel genes related to sports ability, as well as other functions. We analyzed the expressed sequence tags from top half-pipe snowboarding athletes using the SMART complementary DNA (cDNA) library construction method to elucidate the characteristics of the athlete genome and the differential expression of the genes it contains. Overall, we established a full-length cDNA library from the lymphocytes of half-pipe snowboarding athletes and analyzed the inserted gene fragments. We also classified those genes according to molecular function, biological characteristics, cellular composition, protein types, and signal paths. A total of 201 functional genes were noted, which were distributed in 27 pathways. TXN, MDH1, ARL1, ARPC3, ACTG1, and other genes measured in sequence may be associated with physical ability. This suggests that the SMART cDNA library constructed from the genetic material from top athletes is an effective tool for preserving genetic sports resources and providing genetic markers of physical ability for athlete selection.

分类号：

  • 相关文献

[1]Data from expressed sequence tags from the organs and embryos of parthenogenetic Haemaphysalis longicornis. Rika Umemiya-Shirafuji,Jinlin Zhou,Min Liao,Badgar Battsetseg,Damdinsuren Boldbaatar,Takeshi Hatta,Thasaneeya Kuboki,Takeshi Sakaguchi,Huey Shy Chee,Takeharu Miyoshi,Xiaohong Huang,Naotoshi Tsuji,Xuenan Xuan,Kozo Fujisaki. 2021

[2]In vivo transcription of bovine leukemia virus and bovine immunodeficiency-like virus. Wu, DL,Murakami, K,Morooka, A,Jin, H,Inoshima, Y,Sentsui, H. 2003

[3]Boron Affects Immune Function Through Modulation of Splenic T Lymphocyte Subsets, Cytokine Secretion, and Lymphocyte Proliferation and Apoptosis in Rats. Jin, Erhui,Li, Shenghe,Ren, Man,Hu, Qianqian,Gu, Youfang,Li, Shenghe,Li, Kui.

[4]Humoral and cellular immune responses to Fasciola gigantica experimental infection in buffaloes. Zhang, WY,Moreau, E,Yang, BZ,Li, ZQ,Hope, JC,Howard, CJ,Huang, WY,Chauvin, A. 2006

[5]Identification and primary application of hybridomas cell secreting monoclonal antibodies against mink (Neovison vison) interferon-gamma. Hailing Zhang,Shasha Zhang,Sining Fan,Lei Zhang,Bo Hu,Xue Bai,Dongliang Zhang,Rongguang Lu,Jianjun Zhao,Shizhen Lian,Bing Gao,Xijun Yan,Shiying Lu,Yanzhu Zhu. 2022

[6]Analysis of SSRs Information in Capsicum spp. from EST Database. Huang Huan-huan,Zhang Zhong-hua,Zhang Zheng-hai,Mao Sheng-li,Wang Li-hao,Zhang Bao-xi. 2011

[7]Construction of a Full-Length Enriched cDNA Library and Preliminary Analysis of Expressed Sequence Tags from Bengal Tiger Panthera tigris tigris. Liu, Changqing,Lu, Taofeng,Li, Xiangchen,Ma, Yuehui,Guan, Weijun,Liu, Changqing,Guo, Yu,Liu, Dan,Zhang, Minghai,Ma, Jianzhang. 2013

[8]Progress in expressed sequence tags (EST) project of plant genome. Luo, M,Jia, JZ. 2001

[9]PRIMARY ANALYSIS OF THE EXPRESSED SEQUENCE TAGS FROM A FULL-LENGTH ENRICHED cDNA LIBRARY OF SIBERIAN TIGER (PANTHERA TIGRIS ALTAICA). Guo, Y.,Liu, C. Q.,Xu, L. S.,Lu, T. F.,Li, X. C.,Ma, Y. H.,Guan, W. J.,Liu, D.,Ma, J. Z.. 2015

[10]Generation and analysis of a large-scale expressed sequence tags from a full-length enriched cDNA library of Siberian tiger (Panthera tigris altaica). Guo, Yu,Liu, Changqing,Liu, Changqing,Lu, Taofeng,Bai, Chunyu,Li, Xiangchen,Ma, Yuehui,Guan, Weijun,Liu, Dan,Bai, Chunyu.

[11]Analysis of expressed sequence tags (ESTs) from a normalized cDNA library and isolation of EST simple sequence repeats from the invasive cotton mealybug phenacoccus solenopsis. Lang, Kun-Ling,Shen, Chang-Peng,Wan, Fang-Hao,Chu, Dong,Fu, Hai-Bin,Wan, Fang-Hao. 2015

[12]Analysis of Resistance to Powdery Mildew in Wheat Based on Expressed Sequence Tags (EST) Technique. Luo, M,Kong, XY,Jiang, T,Jia, C,Zhou, RH,Jia, JZ.

[13]Porcine skeletal muscle differentially expressed gene CMYA1: isolation, characterization, mapping, expression and association analysis with carcass traits. Xu, X. L.,Xu, X. W.,Pan, P. W.,Yu, M.,Liu, B.,Xu, X. L.,Xu, X. W.,Pan, P. W.,Yu, M.,Liu, B.,Li, K.,Jiang, Z. H.,Rothschild, M. F.,Rothschild, M. F..

[14]Isolation and characterization of induced genes under drought stress at the flowering stage in maize (Zea mays). Li, Hui-Yong,Wang, Tian-Yu,Shi, Yun-Su,Fu, Jun-Jie,Song, Yan-Chun,Wang, Guo-Ying,Li, Yu.

[15]Analysis of ESTs from a Normalized cDNA Library of the Rhizome Tip of Oryza longistaminata. Zhang, Ting,Zhao, Xiuqin,Fu, Binying,Zhang, Ting,Yang, Daichang,Li, Lijuan,Hu, Fengyi.

[16]SSR data mined from expressed sequence tags of phytoparasitic nematodes. Huang, Q. X.,Cheng, X. Y.,Xu, R. M.,Xie, B. Y.. 2010

[17]Construction of cDNA library and preliminary analysis of expressed sequence tags from Siberian tiger. Liu, Chang-Qing,Lu, Tao-Feng,Feng, Bao-Gang,Guan, Wei-Jun,Ma, Yue-Hui,Liu, Chang-Qing,Liu, Dan. 2010

[18]Expressed sequence tags in cultivated peanut (Arachis hypogaea): Discovery of genes in seed development and response to Ralstonia solanacearum challenge. Huang, Jiaquan,Yan, Liying,Lei, Yong,Jiang, Huifang,Ren, Xiaoping,Liao, Boshou.

[19]Generation and characterization of 24 novel EST derived microsatellites from tea plant (Camellia sinensis) and cross-species amplification in its closely related species and varieties. Zhao, Li-Ping,Liu, Zhen,Chen, Liang,Yao, Ming-Zhe,Wang, Xin-Chao.

[20]Differential gene expression of cotton cultivar CCRI24 during somatic embryogenesis. Xiuming Wu,Fuguang Li,Chaojun Zhang,Chuanliang Liu,Xueyan Zhang.

作者其他论文 更多>>