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Biological characterization of liver fatty acid binding gene from miniature pig liver cDNA library

文献类型： 外文期刊

作者： Gao, Y. H.;Wang, K. F.;Zhang, S.;Fan, Y. N.;Guan, W. J.;Ma, Y. H.;Gao, Y. H.;Wang, K. F.;Zhang, S.;Fan, Y. N.

作者机构：

关键词： cDNA library;Hepatocytes;Liver fatty acid binding proteins;Wuzhishan miniature pig

期刊名称： GENETICS AND MOLECULAR RESEARCH

ISSN： 1676-5680

年卷期： 2015 年 14 卷 3 期

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收录情况： SCI

摘要： Liver fatty acid binding proteins (L-FABP) are a family of small, highly conserved, cytoplasmic proteins that bind to long-chain fatty acids and other hydrophobic ligands. In this study, a full-length enriched cDNA library was successfully constructed from Wuzhishan miniature pig, and then the L-FABP gene was cloned from this cDNA library and an expression vector (pEGFP-N3-L-FABP) was constructed in vitro. This vector was transfected into hepatocytes to test its function. The results of western blotting analysis demonstrated that the L-FABP gene from our full-length enriched cDNA library regulated downstream genes, including the peroxisome proliferator-activated receptor family in hepatocytes. This study provides a theoretical basis and experimental evidence for the application of L-FABP for the treatment of liver injury.

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