数字农科院2.0

Current status of genetic transformation technology developed in cucumber (Cucumis sativus L.)

文献类型： 外文期刊

作者： Wang Shun-li;Ku, Seong Sub;Choi, Pil Son;Ye Xing-guo;He Cong-fen;Kwon, Suk Yoon

作者机构：

关键词： Cucumis sativus L.;plant regeneration;genetic transformation;positive selection system

期刊名称： JOURNAL OF INTEGRATIVE AGRICULTURE

ISSN： 2095-3119

年卷期： 2015 年 14 卷 3 期

页码：

收录情况： SCI

摘要： Genetic transformation is an important technique for functional genomics study and genetic improvement of plants. Until now, Agrobacterium-mediated transformation methods using cotyledon as explants has been the major approach for cucumber, and its frequency has been up to 23%. For example, significantly enhancement of the transformation efficiency of this plant species was achieved from the cotyledon explants of the cultivar Poinsett 76 infected by Agrobacterium strains EHA105 with efficient positive selection system in lots of experiments. This review is to summarize some key factors influencing cucumber regeneration and genetic transformation, including target genes, selection systems and the ways of transgene introduction, and then to put forward some strategies for the increasing of cucumber transformation efficiency. In the future, it is high possible for cucumber to be potential bioreactor to produce vaccine and biomaterials for human beings.

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