数字农科院2.0

Effects of Environmental Temperature on the Regeneration Frequency of the Immature Embryos of Wheat (Triticum aestivum L.)

文献类型： 外文期刊

作者： Wang Xin-min;Yin Gui-xiang;Wang Ke;Du Li-pu;Xu Hui-jun;Ye Xing-guo;Ren Xian;Li Jia-rui

作者机构：

关键词： wheat;immature embryos;environmental temperature;preservation;plant regeneration

期刊名称： JOURNAL OF INTEGRATIVE AGRICULTURE

ISSN： 2095-3119

年卷期： 2014 年 13 卷 4 期

页码：

收录情况： SCI

摘要： The immature embryos (IEs) of wheat are the most widely used tissues for in vitro culture and genetic transformation due to its high regeneration competency. However, this explant can only be maintained in 4 degrees C daily cooler for a short period time for its use in plant tissue culture or transformation experiments. This study aimed to investigate the effects of environmental temperature, cryopreservation storage temperature, and heat shock culture (HSC) temperature on the regeneration frequency of wheat IEs. Results indicated that environmental temperature significantly affected the induction of embryonic calli. The optimum total accumulated temperature (TAT) during the time of anthesis and sampling for regeneration of these tissues was around 280 degrees C for spring wheat type cv. CB037 and approximately 300 degrees C for winter wheat type cv. Kenong 199. Regeneration ability obviously declined when the highest environmental temperature was over 35 degrees C for 1 d or a high temperature between 30 and 33 degrees C lasted for 5 d during anthesis and sampling. This finding was verified by culturing the freshly isolated IEs under different temperatures from 29 to 37 degrees C in different controlled growth incubators for 5 d; the IEs almost completely lost regeneration ability when the temperature rose to 37 degrees C. Cryopreservation of -20 degrees C caused the wheat samples lost ability of producing callus or embryonic callus in a few days, and cryopreservation of -10 degrees C more than 10 d made the regeneration potential of the tissues dramatically declined. Comparatively, the temperature that best maintained high regeneration ability was -5 degrees C, at which the materials can be maintained for around 1 mon. In addition, the preservation of the immature samples at -5 or -10 degrees C inhibited the direct germination of the IEs, avoiding the embryo axis removing process. Our results are useful for ensuring that field collection and cryopreservation of the wheat IEs are done correctly to enable tissue culture and generic transformation.

分类号：

  • 相关文献

[1]Improvement of Plant Regeneration from Immature Embryos of Wheat Infected by Agrobacterium tumefaciens. Tao Li-li,Yin Gui-xiang,Du Li-pu,Shi Zheng-yuan,She Mao-yun,Xu Hui-jun,Ye Xing-guo. 2011

[2]Effects of inter-culture, arabinogalactan proteins, and hydrogen peroxide on the plant regeneration of wheat immature embryos. Zhang Wei,Wang Xin-min,Yin Gui-xiang,Wang Ke,Du Li-pu,Xiao Le-le,Ye Xing-guo,Fan Rong. 2015

[3]充分发掘保护利用生物多样性;促进农业可持续发展——《生物多样性与小麦改良》简评. 李祥洲. 1997

[4]Response of rectal temperature of broiler chickens to thermal environment factors. Lin, H,Buyse, J,Du, R,Gu, XH,Zhang, ZY. 2004

[5]30个小麦新品系抗条锈病基因分析及成株期抗病性评价. 冯晶,蔺瑞明,林凤,徐世昌. 2013

[6]小麦抗菌蛋白编码基因BS108的克隆及其转基因抗病小麦的创制. 李钊,周淼平,张增艳,庄洪涛,徐惠君,杜丽璞,叶兴国. 2010

[7]渗透调节物质及有机附加物对小麦体细胞胚发生影响的研究(英文). 孙朝霞,侯思宇,王玉国. 2008

[8]普通小麦品种辐射敏感性模糊聚类分析<英文>. 冯志杰,王琳清. 1992

[9]基因枪转化小麦主要轰击参数的优化. 闵东红,何莎,张彦,夏兰琴. 2013

[10]小麦矮缩病毒分子群体遗传结构研究. 刘艳,王锡锋,周广和. 2008

[11]Optimization of in vitro responses of various explants sources in sorghum (Sorghum bicolor). Rana Imtiaz Ahmed,Shoaib Ur Rehman,Lal Hussain Akhtar,Abdul Majid Khan,Khalid Mahmood,Rana Tauqeer Ahmad,Wajiha Anum. 2022

[12]GREEN PLANT-REGENERATION FROM PROTOPLASTS OF BARLEY (HORDEUM-VULGARE L). YAN, QS,ZHANG, XQ,SHI, JB,LI, JM. 1991

[13]Current status of genetic transformation technology developed in cucumber (Cucumis sativus L.). Wang Shun-li,Ku, Seong Sub,Choi, Pil Son,Ye Xing-guo,He Cong-fen,Kwon, Suk Yoon. 2015

[14]The Influence of Plant Growth Regulators and Light Quality on Somatic Embryogenesis in China Rose (Rosa chinensis Jacq.). Chen, Ji-Ren,Wu, Lian,Hu, Bo-Wen,Yi, Xing,Deng, Zi-Niu,Xiong, Xing-Yao,Liu, Rong,Deng, Zi-Niu,Xiong, Xing-Yao,Xiong, Xing-Yao. 2014

[15]Anther culture and plant regeneration of cotton (Gossypium klotzschianum Anderss). Feng, R,Li, XH,Li, FL. 1996

[16]Highly efficient plant regeneration through somatic embryogenesis in 20 elite commercial cotton (Gossypium hirsutum L.) cultivars. Baohong Zhang,Qinglian Wang,Fang Liu,Kunbo Wang,Taylor P. Frazier. 2009

[17]A rapid shoot regeneration protocol from the cotyledons of hemp (Cannabis sativa L.). Cheng Chaohua,Zang Gonggu,Zhao Lining,Gao Chunsheng,Tang Qing,Chen Jianhua,Su Jianguang,Guo Xinbo,Cheng Chaohua,Peng Dingxiang.

[18]Plant regeneration via somatic embryogenesis in cotton. Bao-Hong Zhang,Fang Liu,Chang-Bing Yao. 2000

[19]Comparisons between Plant and Animal Stem Cells Regarding Regeneration Potential and Application. Lulu Liu,Lu Qiu,Yaqian Zhu,Lei Luo,Xinpei Han,Mingwu Man,Fuguang Li,Maozhi Ren,Yadi Xing. 2023

[20]Adventitious embryonic causal gene FhRWP regulates multiple developmental phenotypes in citrus reproduction. Song, Xietian,Wang, Nan,Zhou, Yin,Tian, Xiaoyu,Xie, Zongzhou,Chai, Lijun,Wu, Xiaomeng,Xu, Qiang,Zhang, Fei,Ye, Junli,Deng, Xiuxin. 2024

作者其他论文 更多>>