数字农科院2.0

Plant Polysaccharides Modulate Biofilm Formation and Insecticidal Activities of Bacillus thuringiensis Strains

文献类型： 外文期刊

作者： Mengmeng Li;Changlong Shu;Wang Ke;Xiaoxiao Li;Yiyan Yu;Xiong Guan;Tianpei Huang

作者机构：

关键词： Bacillus thuringiensis;biofilm;insecticidal activity;pectin;plant polysaccharide;Plutella xylostella;xylan

期刊名称： Frontiers in Microbiology

ISSN： 1664-302X

年卷期： 2021 年 12.0 卷

页码：

收录情况： JCR（2021版）

摘要： After the biological pesticide Bacillus thuringiensis (Bt) is applied to the field, it has to remain on the surface of plants to have the insecticidal activities against insect pests. Bt can form biofilms on the surface of vegetable leaves, which were rich in polysaccharides. However, the relationship between polysaccharides of the leaves and the biofilm formation as well as the insecticidal activities of Bt is still unknown. Herein, this study focused on the effects of plant polysaccharides pectin and xylan on biofilm formation and the insecticidal activities of Bt strains. By adding pectin, there were 88 Bt strains with strong biofilm formation, 69 strains with weak biofilm formation, and 13 strains without biofilm formation. When xylan was added, 13 Bt strains formed strong biofilms, 98 strains formed weak biofilms, and 59 strains did not form biofilms. This indicated that two plant polysaccharides, especially pectin, modulate the biofilm formation of Bt strains. The ability of pectin to induce biofilm formation was not related to Bt serotypes. Pectin promoted the biofilms formed by Bt cells in the logarithmic growth phase and lysis phase at the air–liquid interface, while it inhibited the biofilms formed by Bt cells in the sporangial phase at the air–liquid interface. The dosage of pectin was positively correlated with the yield of biofilms formed by Bt cells in the logarithmic growth phase or lysis phase at the solid–liquid interfaces. Pectin did not change the free-living growth and the cell motility of Bt strains. Pectin can improve the biocontrol activities of the spore–insecticidal crystal protein mixture of Bt and BtK commercial insecticides, as well as the biofilms formed by the logarithmic growth phase or lysis phase of Bt cells. Our findings confirmed that plant polysaccharides modulate biofilm formation and insecticidal activities of Bt strains and built a foundation for the construction of biofilm-type Bt biopesticides.

分类号：

  • 相关文献

[1]Cloning and characterization of a novel Cry1A toxin from Bacillus thuringiensis with high toxicity to the Asian corn borer and other lepidopteran insects. Liang, Gemei,Li, Haitao,He, Kanglai,Song, Fuping,Feng, Xue,Zhang, Jie,Crickmore, Neil,Huang, Dafang.

[2]Purification of an active fragment of Cry1Ie toxin from Bacillus thuringiensis. Zhang, Chunlu,Lin, Xiaoyin,Zhang, Yanrui,He, Kanglai,Song, Fuping,Zhang, Jie.

[3]Cyclosporin A acts as a novel insecticide against Cry1Ac-susceptible and -resistant Helicoverpa armigera. Wei J.,Liu S.,Wang K.,Sun C.,Li S.,Liu X.,Yin X.,Bai S.,Liang G.,Crickmore N.,An S.. 2022

[4]Identification and functional characterization of eight novel tpp family genes from Bacillus thuringiensis. Cao, Beibei,Sun, Xiaoni,Shu, Changlong,Geng, Lili,Zhang, Jie. 2023

[5]The midgut cadherin-like gene is not associated with resistance to Bacillus thuringiensis toxin Cry1Ac in Plutella xylostella (L.). Guo, Zhaojiang,Kang, Shi,Zhu, Xun,Wu, Qingjun,Wang, Shaoli,Xie, Wen,Zhang, Youjun.

[6]Influences of Cry1Ac Broccoli on Larval Survival and Oviposition of Diamondback Moth. Yi, Dengxia,Cui, Shusong,Yang, Limei,Fang, Zhiyuan,Liu, Yumei,Zhuang, Mu,Zhang, Yangyong,Yi, Dengxia. 2015

[7]Proteomics-based identification of midgut proteins correlated with Cry1Ac resistance in Plutella xylostella (L.). Xia, Jixing,Guo, Zhaojiang,Yang, Zezhong,Zhu, Xun,Kang, Shi,Yang, Xin,Wu, Qingjun,Wang, Shaoli,Xie, Wen,Zhang, Youjun,Xia, Jixing,Xu, Weijun,Yang, Fengshan.

[8]High resistance of transgenic cabbage plants with a synthetic cry1Ia8 gene from Bacillus thuringiensis against two lepidopteran species under field conditions. Yi, Dengxia,Yang, Weijie,Tang, Jun,Wang, Li,Fang, Zhiyuan,Liu, Yumei,Zhuang, Mu,Zhang, Yangyong,Yang, Limei,Yi, Dengxia.

[9]Determining the involvement of two aminopeptidase Ns in the resistance of Plutella xylostella to the Bt toxin Cry1Ac: Cloning and study of in vitro function. Chang, Xiaoli,Wu, Qingjun,Wang, Shaoli,Wang, Ran,Yang, Zhongxia,Chen, Defeng,Jiao, Xiaoguo,Mao, Zhenchuan,Zhang, Youjun. 2012

[10]The Minimal Active Fragment of the Cry1Ai Toxin is Located Between 36(I) and 605(I). Lin Hui-yan,Li Ying,Shu Chang-long,Song Fu-ping,Zhang Jie. 2014

[11]Down-regulation of a novel ABC transporter gene (Pxwhite) is associated with Cry1Ac resistance in the diamondback moth, Plutella xylostella (L.). Guo, Zhaojiang,Kang, Shi,Zhu, Xun,Xia, Jixing,Wu, Qingjun,Wang, Shaoli,Xie, Wen,Zhang, Youjun.

[12]Screening of cry-type promoters with strong activity and application in Cry protein encapsulation in a sigK mutant. Zheng, Qingyun,Peng, Qi,Du, Lixin,Shu, Changlong,Zhang, Jie,Song, Fuping,Zhou, Changmei.

[13]Identification of the minimal active fragment of the Cry1Ah toxin. Zhou, Zishan,Song, Fuping,Shu, Changlong,Zhang, Jie,Huang, Dafang.

[14]A cis-acting mutation in the pxabcg1 promoter is associated with cry1ac resistance in plutella xylostella (l.). Jianying Qin,Fan Ye,Linzheng Xu,Xuguo Zhou,Neil Crickmore,Xiaomao Zhou,Youjun Zhang,Zhaojiang Guo. 2021

[15]A versatile contribution of both aminopeptidases N and ABC transporters to Bt Cry1Ac toxicity in the diamondback moth. Dan Sun,Liuhong Zhu,Le Guo,Shaoli Wang,Qingjun Wu,Neil Crickmore,Xuguo Zhou,Alejandra Bravo,Mario Soberón,Zhaojiang Guo,Youjun Zhang. 2022

[16]A cis-acting mutation in the pxabcg1 promoter is associated with cry1ac resistance in plutella xylostella (l.). Jianying Qin,Fan Ye,Linzheng Xu,Xuguo Zhou,Neil Crickmore,Xiaomao Zhou,Youjun Zhang,Zhaojiang Guo. 2021

[17]MAPK-Activated Transcription Factor PxJun Suppresses PxABCB1 Expression and Confers Resistance to Bacillus thuringiensis Cry1Ac Toxin in Plutella xylostella (L.). Jianying Qin,Le Guo,Fan Ye,Shi Kang,Dan Sun,Liuhong Zhu,Yang Bai,Zhouqiang Cheng,Linzheng Xu,Chunzheng Ouyang,Lifeng Xiao,Shaoli Wang,Qingjun Wu,Xuguo Zhou,Neil Crickmore,Xiaomao Zhou,Zhaojiang Guo,Youjun Zhang. 2021

[18]MAP4K4 controlled transcription factor POUM1 regulates PxABCG1 expression influencing Cry1Ac resistance in Plutella xylostella (L.). Linzheng Xu,Jianying Qin,Wei Fu,Shaoli Wang,Qinjun Wu,Xuguo Zhou,Neil Crickmore,Zhaojiang Guo,Youjun Zhang. 2022

[19]Retrotransposon-mediated evolutionary rewiring of a pathogen response orchestrates a resistance phenotype in an insect host. Zhaojiang Guo,Le Guo,Yang Bai,Shi Kang,Dan Sun,Jianying Qin,Fan Ye,Shaoli Wang,Qingjun Wu,Wen Xie,Xin Yang,Neil Crickmore,Xuguo Zhou,Youjun Zhang. 2023

[20]CRISPR/Cas9-based functional characterization of PxABCB1 reveals its roles in the resistance of Plutella xylostella (L.) to Cry1Ac, abamectin and emamectin benzoate. Chun zheng OUYANG,Fan YE,Qing jun WU,Shao li WANG,Neil CRICKMORE,Xu guo ZHOU,Zhao jiang GUO,You jun ZHANG. 2023

作者其他论文 更多>>