数字农科院2.0

Development and validation of a screening method for actinomycetes from Protaetia brevitarsis larval frass with biocontrol potential

文献类型： 外文期刊

作者： Qi Peng;Lida Zhang;Chang Qi;Lili Geng;Hanxiang Wu;Wensheng Xiang;Jie Zhang;Xiangjing Wang;Changlong Shu

作者机构：

关键词： Actinomycete;Biocontrol;Peanut;Sclerotium rolfsii;Screening method

期刊名称： Biological Control

ISSN： 1049-9644

年卷期： 2026 年 213 卷

页码：

收录情况： SCIE（2025版）

摘要： This study aimed to establish an efficient screening method for identifying biocontrol strains effective against soil-borne pathogens of plants. To achieve this objective, we developed a quantitatively-controlled potted plant testing (QC-PPT) system by optimizing cultivation devices, growth substrates, pathogen inoculation methods, and quantitative evaluation of root infection. This design effectively confines the roots and facilitates uniform pathogen infection. The optimal inoculation timing was determined to be between the 10th and 16th day of growth. Furthermore, a substrate composed of vermiculite with 4% organic compost was identified as ideal, supporting vigorous peanut growth while allowing sufficient pathogen infection required for reliable biocontrol evaluation. Using this system, seven strains with strong antagonistic effects against Sclerotium rolfsii were isolated from White-spotted Flower Chafer (WSFC, Protaetia brevitarsis) larval frass. In vitro assays showed that strain X13 inhibited Sclerotium rolfsii growth by 44.56%, while strain X15 performed more excellently in the QC-PPT system: it increased peanut root dry weight by 32.8% and reduced root lesion area by 51.2% compared to the control group. Genomic sequencing data revealed that the superior strain X15 possesses the most diverse set of secondary metabolite biosynthetic gene clusters. Collectively, strain X15 is a promising candidate for biopesticide development, and the QC-PPT system we established can be extended to other crop-soil-borne pathogen systems. This study not only provides an effective biocontrol resource for peanut southern blight but also facilitates the development of sustainable disease management strategies in agriculture.

分类号：

  • 相关文献

[1]Metabolites Discovery from Streptomyces xanthus: Exploring the Potential of Desert Microorganisms. Xinrong Luo,Zhanwen Liu,Zhanfeng Xia,Xiaoxia Luo,Juan Zhang,Ailiang Chen,Haoxin Wang,Chuanxing Wan,Lili Zhang. 2025

[2]Integrated proteomic and metabolomic analyses reveal significant changes in chloroplasts and mitochondria of pepper (Capsicum annuum L.) during Sclerotium rolfsii infection. Liao, Hongdong,Wen, Xiangyu,Deng, Xuelei,Wu, Yonghong,Xu, Jianping,Li, Xin,Zhou, Shudong,Li, Xuefeng,Zhu, Chunhui,Luo, Feng,Ma, Yanqing,Zheng, Jingyuan. 2022

[3]Identification of the Isocitrate Lyase SrICL as a Potential Target Protein of Butylidenephthalide in Sclerotium rolfsii via Drug Affinity Responsive Target Stability and Metabolomics. Mao, Xuewei,Chen, Liting,Wang, Meizi,Cao, Tangbo,Cui, Kaidi,Zhang, Guoyan,Cao, Lidong,Zhang, Jingjing,Zhou, Lin. 2025

[4]Multi-Residue Method for the Screening of Benzimidazole and Metabolite Residues in the Muscle and Liver of Sheep and Cattle Using HPLC/PDAD with DVB-NVP-SO3Na for Sample Treatment. Xiong, Lin,Shimo, Shimo Peter,Li, Weihong,Yang, Xiaolin,Yan, Ping,Xiong, Lin,Shimo, Shimo Peter,Li, Weihong,Yang, Xiaolin,Yan, Ping,Huang, Lele.

[5]One simple and rapid spectrophotometric method for screening lipoxygenase-3-null in rice embryos. Shen, WB,Zhou, T,Wang, YH,Yu, WW,Zheng, TQ,Zhai, HQ,Wan, JM. 2002

[6]On-site screening method for bioavailability assessment of the organophosphorus pesticide, methyl parathion, and its primary metabolite in soils by paper strip biosensor. Zhao Ma,Yuanbo Li,Chao Lu,Meng Li. 2023

[7]Rhizosphere-Associated Alcaligenes And Bacillus Strains T.hat Induce Resistance Against B last And Sheath Blight Diseases, Enhance Plant Growth And Improve Mineral Content In Rice. Kakar, K. U.,Kakar, K. U.,Cui, Z.,Cui, Z.,Nawaz, Z.,Shu, Qing. -Yao.,Almoneafy, A. A.,Ullah, R.. 2018

[8]Interactions in the biological control of western flower thrips Frankliniella occidentalis (Pergande) and two-spotted spider mite Tetranychus urticae Koch by the predatory bug Orius insidiosus Say on beans. Borgemeister, C,Poehling, HM. 2006

[9]Chinese leek (Allium tuberosum Rottler ex Sprengel) reduced disease symptom caused by root-knot nematode. Huang Yong-hong,Huang Yong-hong,Mao Zhen-chuan,Xie Bing-yan.

[10]A new mycoparasite, Aspergillus sp ASP-4, parasitizes the sclerotia of Sclerotinia sclerotiorum. Hu, Xiaojia,Xie, Lihua,Yu, Changbing,Li, Yinshui,Liao, Xing,Webster, Gordon.

[11]Suppression of Meloidogyne incognita by the endophytic fungus Acremonium implicatum from tomato root galls. Tian, Xueliang,Tian, Xueliang,Yao, Yurong,Chen, Guohua,Mao, Zhenchuan,Xie, Bingyan,Wang, Xiaotian.

[12]Transformation of the endophytic fungus Acremonium implicatum with GFP and evaluation of its biocontrol effect against Meloidogyne incognita. Yao, Yu-Rong,Tian, Xue-Liang,Shen, Bao-Ming,Mao, Zhen-Chuan,Chen, Guo-hua,Xie, Bing-Yan,Tian, Xue-Liang,Shen, Bao-Ming.

[13]A strain of Aspergillus flavus from China shows potential as a biocontrol agent for aflatoxin contamination. Zhou, Lu,Wei, Dan-Dan,Selvaraj, Jonathan Nimal,Shang, Bo,Zhang, Chu-Shu,Xing, Fu-Guo,Zhao, Yue-Ju,Wang, Yan,Liu, Yang.

[14]Inhibitory Action of Biofungicide Physcion on Initial and Secondary Infection of Magnaporthe oryzae. Wang, Na,Cai, Mengling,Wang, Xin,Xie, Yaqiong,Ni, Hanwen,Wang, Na.

[15]Genome sequence of Pseudomonas parafulva CRS01-1, an antagonistic bacterium isolated from rice field. Liu, Qunen,Zhang, Yingxin,Yu, Ning,Bi, Zhenzhen,Zhu, Aike,Zhan, Xiaodeng,Wu, Weixun,Yu, Ping,Chen, Daibo,Cheng, Shihua,Cao, Liyong,Zhang, Yingxin,Zhan, Xiaodeng,Wu, Weixun,Yu, Ping,Chen, Daibo,Cheng, Shihua,Cao, Liyong.

[16]Nutritional requirements of mycelial growth and sporulation of several biocontrol fungi in submerged and on solid culture. Liu, Xingzhong,Gao, Li.

[17]Environment polluting conventional chemical control compared to an environmentally friendly IPM approach for control of diamondback moth, Plutella xylostella (L.), in China: a review. Jin, Fengliang,Farooq, Muhammad,Nasim, Wajid,Nasim, Wajid,Nasim, Wajid,Akram, Waseem,Khan, Fawad Zafar Ahmad,Jaleel, Waqar,Zhu, Xun,Yin, Haichen,Fahad, Shah,Chauhan, Bhagirath Singh.

[18]The effects of the combination of Pichia membranefaciens and BTH on controlling of blue mould decay caused by Penicillium expansum in peach fruit. Cao, Shifeng,Hu, Zhichao,Yang, Zhenfeng,Zheng, Yonghua.

[19]Insights into the Biocontrol Function of a Burkholderia gladioli Strain against Botrytis cinerea. Wang, Dan,Luo, Wan-Zhen,Zhang, Dan-Dan,Li, Ran,Kong, Zhi-Qiang,Song, Jian,Dai, Xiao-Feng,Alkan, Noam,Chen, Jie-Yin. 2023

[20]Genome Sequence of Pseudomonas koreensis CRS05-R5, an Antagonistic Bacterium Isolated from Rice Paddy Field. Haiyan Lin , Shikai Hu +, Ruifang Liu , Ping Chen , Changwei Ge , Bo Zhu , Longbiao Guo *. 2016

作者其他论文 更多>>