数字农科院2.0

Purification And Characteristics Of Individual Major Royal Jelly Protein 1-3

文献类型： 外文期刊

作者： Wang, XY; Dong, J; Qiao, JT; Zhang, GS; Zhang, HC

作者机构：

关键词： Mrjps; Purity; Yield; Mrjp1 Oligomers; Secondary Structure

期刊名称： JOURNAL OF APICULTURAL RESEARCH

ISSN： 0021-8839

年卷期： 2020 年 59 卷 5 期

页码：

收录情况： JCR（2021版）

摘要： Major royal jelly proteins (MRJPs) are deemed to the most characteristic and abundant constituents in royal jelly. The present paper establishes a sequential process to purify the natural MRJP 1-3 by three different chromatographic procedures. Five individual proteins were obtained, including MRJP 1 oligomer 1, MRJP 1 oligomer 2, MRJP 1 monomer, MRJP 2 and MRJP 3s (contain three MRJP 3 variants) with a purity of 89.88%, 67.79%, 99.92%, 99.41%, and 99.97%, respectively. This is the first report that MRJP 1 mainly possesses three oligomers: a 228 kDa MRJP 1 oligomer 1, a 408 kDa MRJP 1 oligomer 2 and a 639 kDa MRJP 1 oligomer 3. We infer that MRJP 1 oligomer 3 (639 kDa) may be assembled by MRJP 1 oligomer 1 (228 kDa) and MRJP 1 oligomer 2 (408 kDa). The secondary structure of the purified MRJP 1-3 consisted predominantly of beta-sheets and random coil in the native conformation. Our results will contribute to the physiological and physicochemical function of the purified individual MRJPs.

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