Cytotoxicity induced by ochratoxin A, zearalenone, and alpha-zearalenol: Effects of individual and combined treatment
文献类型: 外文期刊
作者: Wang, H. W.;Wang, J. Q.;Li, S. L.;Zhang, Y. D.;Zheng, N.;Wang, H. W.;Li, F. D.;Wang, H. W.;Wang, J. Q.;Li, S. L.;Zhang, Y. D.;Zheng, N.;Zheng, B. Q.
作者机构:
关键词: Ochratoxin A;Zearalenone;alpha-Zearalenol;Isobologram;Hep G2;Cytotoxicity
期刊名称: FOOD AND CHEMICAL TOXICOLOGY
ISSN: 0278-6915
年卷期: 2014 年 71 卷
页码:
收录情况: SCI
摘要: This study investigated the cytotoxicity of combined mycotoxins of ochratoxin A (OTA), zearalenone (ZEA), anclior alpha-zearalenol (alpha-ZOL). The cytotoxicity of two mycotoxin combinations (two two-toxin combinations and one three-toxin combination) on human Hep G2 cells was evaluated using a tetrazolium salt (MTT) assay and isobologram analysis. Our results demonstrated significant cytotoxic effects of the two-toxin combination and the three-toxin combination on Hep G2 cells in a time- and concentration-dependent manner. The combination indexes (CI) were 2.73-7.67 for the OTA + ZEA combination and 1.23-17.82 for the OTA + alpha-ZOL combination after 24 h, 48 h, and 72 h of exposure at all inhibit concentration (IC) levels (IC10-IC90), indicating an antagonism. The CIs of the ZEA + alpha-ZOL combination were 1.29-2.55 after 24 h and 72 h of exposure (IC10-IC90), indicating an antagonism. The CIs of the ZEA + alpha-ZOL combination were 0.74-1.68 after 48 h of exposure, indicating synergism (IC80-IC90), additive effects (IC50-IC70), or antagonism (IC10-IC40). For the OTA + ZEA + alpha-ZOL combination, the CIs were 1.41-14.65 after 24 h, 48 h, and 72 h of exposure (IC10-IC90), indicating an antagonism. (C) 2014 Elsevier Ltd. All rights reserved.
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