数字农科院2.0

The Y123H substitution perturbs FvCYP51B function and confers prochloraz resistance in laboratory mutants of Fusarium verticillioides

文献类型： 外文期刊

作者： Chen, F.;Diao, Y.;Liu, X.;Fan, J.;Chen, F.;Cools, H. J.;Kelly, S. L.;Kelly, S. L.

作者机构：

关键词： azole fungicide;CYP51;resistance mechanism;site mutation;yeast expression

期刊名称： PLANT PATHOLOGY

ISSN： 0032-0862

年卷期： 2014 年 63 卷 4 期

页码：

收录情况： SCI

摘要： Fusarium verticillioides reduces corn yield and contaminates infected kernels with the toxin fumonisin, which is harmful to humans and animals. Previous research has demonstrated that F. verticillioides can be controlled by the azole fungicide prochloraz. Currently, prochloraz is used as a foliar spray to control maize disease in China, which will increase the risk of resistance. Although F. verticillioides resistance to prochloraz has not been reported in the field, possible resistance risk and mechanisms resulting in prochloraz resistance were explored in the laboratory. Four prochloraz-resistant strains of F. verticillioides were generated by successive selection on fungicide-amended media. The mycelial growth rates of the mutants were inversely related to the level of resistance. All four mutants were cross-resistant to the triazole fungicides triadimefon, tebuconazole and difenoconazole, but not to the multisite fungicide chlorothalonil or to the MAP/histidine-kinase inhibitor fungicide fludioxonil. Based on the Y123H mutation in FvCYP51B, the four resistant mutants were subdivided into two genotypes: PCZ-R1 mutants with wildtype FvCYP51B and PCZ-R2 mutants with substitution Y123H in FvCYP51B. Wildtype FvCYP51B complemented the function of native ScCYP51 in Saccharomyces cerevisiae YUG37::erg11, whereas Y123H-mutated FvCYP51B did not. For the PCZ-R1 mutants, induced expression of FvCYP51A increased resistance to prochloraz. For the PCZ-R2 mutants, disruption of FvCYP51B function by the Y123H substitution caused constitutive up-regulation of FvCYP51A expression and thus resistance to prochloraz.

分类号：

  • 相关文献

[1]小立碗藓细胞色素P450基因PpCYP51G1的分离及转化载体构建. 丁谦,叶帅,李景娟,朱正歌,高建伟. 2013

[2]Targeted deletion of three CYP51s in Fusarium fujikuroi and their different roles in determining sensitivity to 14a-demethylase inhibitor fungicides. Mao, Cheng-Xin,Luo, Ju,Zhang, Yu,Zhang, Chuan-Qing. 2023

[3]Identification and haplotype analysis of the gene SiLfl1 regulate leaf length in foxtail millet. Yannan Wang,Han Jia,Linlin Zhang,Hui Zhi,Guanqing Jia,Xianmin Diao,Sha Tang. 2025

[4]Enhanced expression of the E-rns protein of classical swine fever virus in yeast and its application in an indirect enzyme-linked immunosorbent assay for antibody differentiation of infected from vaccinated animals. Luo, Yuzi,Li, Lin,Dong, Mei,Xu, Jingjing,Shao, Lina,Lei, Jianlin,Li, Na,He, Wen-Rui,Zhao, Bibo,Li, Su,Li, Yongfeng,Sun, Yuan,Qiu, Hua-Ji,Austermann-Busch, Sophia,Becher, Paul,Liu, Lihong,Luo, Yuzi,Li, Lin,Xu, Jingjing,Shao, Lina,Lei, Jianlin,He, Wen-Rui,Zhao, Bibo,Li, Su,Li, Yongfeng,Liu, Lihong,Sun, Yuan,Qiu, Hua-Ji.

[5]A xylanase with broad pH and temperature adaptability from Streptomyces megasporus DSM 41476, and its potential application in brewing industry. Qiu, Zhenhua,Shi, Pengjun,Luo, Huiying,Bai, Yingguo,Yuan, Tiezheng,Yang, Peilong,Yao, Bin,Qiu, Zhenhua,Liu, Suchun.

[6]Isolation and Functional Characterization of a Novel Gene Encoding a Dehydration Responsive Element Binding Transcription Factor from Populus euphratica. Wei, Li,Wang, Suomin,Ma, Jiangtao,Wu, Yanmin,Ma, Jiangtao.

[7]Antiviral activity of mink interferon alpha expressed in the yeast Pichia pastoris. Hailing Zhang,Dongliang Zhang,Han Lu,Deying Zou,Bo Hu,Shizhen Lian,Shiying Lu. 2022

[8]Identification of Δ9 and Δ11 Desaturases Involved in Sex Pheromone Biosynthesis in Mythimna loreyi (Lepidoptera: Noctuidae). Wang, Liuyang,Guo, Pei,Zhang, Xiaofang,Duan, Yun,Ning, Jun,Zhang, Tao,Yang, Xinling. 2025

[9]Metabolic resistance in Nilaparvata lugens to etofenprox, a non-ester pyrethroid insecticide. Sun, Huahua,Yang, Baojun,Zhang, Yixi,Liu, Zewen,Yang, Baojun.

[10]Efficient antibacterial agents: A review of the synthesis, biological evaluation and mechanism of pleuromutilin derivatives. Shang, Ruofeng,Guo, Wenzhu,Liang, Jianping,Wang, Jiatu.

[11]Characterization of imidacloprid resistance in the housefly Musca domestica (Diptera: Muscidae). Wang, Qingmin,Gao, Xiwu,Zhang, Lan. 2012

[12]Cotton bollworm resistance to Bt transgenic cotton: A case analysis. Li YunHe,Gao YuLin,Ning ChangMing,Wu KongMing. 2010

[13]Fipronil resistance in the whitebacked planthopper (Sogatella furcifera): possible resistance mechanisms and cross-resistance. Li, Jian,Shao, Ying,Liu, Zewen,Tang, Jian,Yang, Baojun.

[14]Molecular cloning of heat shock protein gene HSP90 and effects of abamectin and double-stranded RNA on its expression in Panonychus citri (Trombidiformes: Tetranychidae). Tian, Huixia,Yu, Shijiang,Yue, Jiansu,Ran, Chun,Yang, Juan,Ding, Lili,Liu, Haoqiang,Li, Hongjun,Cong, Lin,Ran, Chun,Liu, Bin,Chen, Fei.

[15]Cross-resistance and biochemical mechanisms of abamectin resistance in the western flower thrips, Frankliniella occidentalis. Chen, Xuelin,Yuan, Linze,Du, Yuzhou,Wang, Jianjun,Zhang, Youjun. 2011

[16]Transcriptome analysis of resistant and susceptible mulberry responses to Meloidogyne enterolobii infection. Hudie Shao,Yu Fu,Pan Zhang,Chunping You,Chuanren Li,Huan Peng. 2021

[17]Field-evolved resistance to 11 insecticides and the mechanisms involved in Helicoverpa armigera (Lepidoptera: Noctuidae). Qinqin Wang,Changhui Rui,Li Wang,Shahzad Ali Nahiyoon,Weiling Huang,Junshu Zhu,Xuejiao Ji,Qingjie Yang,Huizhu Yuan,Li Cui. 2021

[18]Interactions between Verticillium dahliae and cotton: pathogenic mechanism and cotton resistance mechanism to Verticillium wilt. Zhu, Yutao,Zhao, Mei,Li, Taotao,Wang, Lianzhe,Liao, Chunli,Liu, Dongxiao,Zhang, Huamin,Zhao, Yanpeng,Liu, Lisen,Ge, Xiaoyang,Li, Bingbing. 2023

[19]Resistance Selection Of Indoxacarb In H.elicoverpa Armigera (Hubner) (Lepidoptera: N octuidae): Cross-Resistance, Biochemical Mechanisms And Associated Fitness Costs. Cui, L,Wang, QQ,Qi, HL,Wang, QY,Yuan, HZ,Rui, CH. 2018

[20]Transcriptome-Based Identification and Characterization of Genes Associated with Resistance to Beta-Cypermethrin in Rhopalosiphum padi (Hemiptera: Aphididae). Qiuchi Li,Xinan Li,Yulin Sun,Xujun Tian,Saige Zhu,Yanbo Wang,Haifeng Gao,Caihua Shi,Xun Zhu. 2023

作者其他论文 更多>>