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Exploring the Electron Transfer Pathway in the Oxidation of Avermectin by CYP107Z13 in Streptomyces ahygroscopicus ZB01

文献类型： 外文期刊

作者： Zhang, Yujie;Zhang, Lin;Yang, Xiaoyan;Jiang, Xiliang

作者机构：

期刊名称： PLOS ONE

ISSN： 1932-6203

年卷期： 2014 年 9 卷 6 期

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收录情况： SCI

摘要： Streptomyces ahygroscopicus ZB01 can effectively oxidize 4 ''-OH of avermectin to form 4 ''-oxo-avermectin. CYP107Z13 is responsible for this site-specific oxidation in ZB01. In the present study, we explored the electron transfer pathway in oxidation of avermectin by CYP107Z13 in ZB01. A putative [3Fe-4S] ferredoxin gene fd68 and two possible NADH-dependent ferredoxin reductase genes fdr18 and fdr28 were cloned from the genomic DNA of ZB01. fd68 gene disruption mutants showed no catalytic activity in oxidation of avermectin to form 4 ''-oxo-avermectin. To clarify whether FdR18 and FdR28 participate in the electron transfer during avermectin oxidation by CYP107Z13, two whole-cell biocatalytic systems were designed in E. coli BL21 (DE3), with one co-expressing CYP107Z13, Fd68 and FdR18 and the other co-expressing CYP107Z13, Fd68 and FdR28. Both of the two biocatalytic systems were found to be able to mediate the oxidation of avermectin to form 4 ''-oxo-avermectin. Thus, we propose an electron transfer pathway NADH -> FdR18/FdR28 -> Fd68 -> CYP107Z13 for oxidation of avermectin to form 4 ''-oxo-avermectin in ZB01.

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