数字农科院2.0

ZmBARK1 as a low-temperature tolerance gene in maize germination

文献类型： 外文期刊

作者： Xu, Qingyu;Song, Yiying;Bao, Di;Meng, Lingzhi;Di, Hong;Zhang, Lin;Dong, Ling;Zeng, Xing;Zhang, Jiayue;Li, Chunxiang;Xing, Jiapeng;Zhang, Naifu;Li, Xin;Weng, Jianfeng;Wang, Zhenhua;Zhou, Yu

作者机构：

关键词： Maize;Low-temperature;GWAS;RNA-seq;ZmBARK1

期刊名称： CROP JOURNAL

ISSN： 2095-5421

年卷期： 2025 年 13 卷 4 期

页码：

收录情况： SCIE（2025版） ; ; CSCD（2025-2026年度） ; ; 科技核心（2024版） ; ; 农林核心（2024版）

摘要： Maize (Zea mays L.), a globally significant cereal crop, is produced in vast quantities worldwide. However, its growth is severely constrained by low temperatures, particularly during seed germination, which significantly impairs seedling emergence. In this study, genetic diversity across six germination-associated phenotypic traits (RGR, RSL, RTL, RRSA, RRV, and RSVI) of 304 inbred lines was analyzed, to evaluate the capacity of these lines for low-temperature tolerance. Genome-wide association study (GWAS) was carried out by combining six germination-associated phenotypic traits and genotypic data from 30-fold resequencing. The gene ZmBARK1 was identified through integrated GWAS and RNA-seq analyses, and its association with low-temperature tolerance during maize germination was validated by quantitative real-time PCR (qRT-PCR). ZmBARK1, encoding BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1, was located on the bin 4.09 region of maize chromosome 4. Amino acid comparison and subcellular localization analyses revealed that ZmBARK1 is highly homologous to AtBAK1 and is localized to the plasma membrane of the cell, which may be involved in regulating brassinosteroid (BR) signaling. In addition, we revealed the role of ZmBARK1 in low-temperature tolerance during maize germination. Compared with wild-type (WT), the ethyl methanesulfonate (EMS) mutant zmbark1 was characterized by substantially enhanced low-temperature tolerance. Overall, these findings provide promising candidate genes, improve low-temperature tolerance in maize, and advance the understanding of regulatory mechanisms underlying maize's response to low-temperature stress. (c) 2025 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NCND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

分类号：

  • 相关文献

[1]Comprehensive analysis of transcriptional data on seed germination of two maize inbred lines under low-temperature conditions. Yinchao Zhang,Peng Liu,Chaoying Zou,Zhong Chen,Guangsheng Yuan,Shibin Gao,Guangtang Pan,Yaou Shen,Langlang Ma. 2023

[2]小麦芒基因定位及其与农艺性状的相关性分析. 李玲,刘盼,张蕾,张浩,贾继增,高丽锋. 2021

[3]Comprehensive transcriptome and WGCNA analysis reveals the potential function of anthocyanins in low-temperature resistance of a red flower mutant tobacco. Yinchao Zhang,Xiaoling Qu,Xiuchun Li,Min Ren,Ying Tong,Xiuming Wu,Yangyang Sun,Fengyan Wu,Aiguo Yang,Shuai Chen. 2023

[4]Genome-Wide Association Analysis Coupled With Transcriptome Analysis Reveals Candidate Genes Related to Salt Stress in Alfalfa (Medicago sativa L.). Fei He,Chunxue Wei,Yunxiu Zhang,Ruicai Long,Mingna Li,Zhen Wang,Qingchuan Yang,Junmei Kang,Lin Chen. 2022

[5]Dissecting the Genetic Mechanisms of Hemicellulose Content in Rapeseed Stalk. Xu, Yinhai,Yang, Yuting,Yu, Wenkai,Liu, Liezhao,Hu, Qiong,Wei, Wenliang,Liu, Jia. 2022

[6]Genome-wide association and RNA-seq analyses identify loci for pod orientation in rapeseed (Brassica napus). Yang Y.,Wang W.,Hu Q.,Raman H.,Liu J.. 2023

[7]Genome-wide association study for phosphate responsive root hair length and density in bread wheat. Saman Maqbool,Fatima Saeed,Ammarah Maqbool,Muhammad Ibrar Khan,Mohsin Ali,Awais Rasheed,Xianchun Xia,Zhonghu He. 2023

[8]Integrating GWAS and transcriptomics to identify candidate genes conferring relative growth rate trait in white-feathered broiler. Peihao Liu,Na Luo,Dawei Liu,Fan Ying,Dan Zhu,Jie Wen,Guiping Zhao,Bingxing An. 2024

[9]Unraveling key genes and pathways involved in Verticillium wilt resistance by integrative GWAS and transcriptomic approaches in Upland cotton. Khan, Majid,Hu, Daowu,Dai, Shuai,Li, Hongge,Peng, Zhen,He, Shoupu,Awais, Muhammad,Du, Xiongming,Geng, Xiaoli. 2025

[10]Unveiling alfalfa root rot resistance genes through an integrative GWAS and transcriptome study. He, Fei,Xu, Ming,Liu, Hao,Xu, Yanchao,Long, Ruicai,Kang, Junmei,Yang, Qingchuan,Chen, Lin. 2025

[11]Genome-Wide Association Integrating a Transcriptomic Meta-Analysis Suggests That Genes Related to Fat Deposition and Muscle Development Are Closely Associated with Growth in Huaxi Cattle. Cheng Li Liu,Tao Ren,Peng Cheng Ruan,Yong Fu Huang,Simone Ceccobelli,De Jun Huang,Lu Pei Zhang,Guang Xin E. 2025

[12]Domestication of Tartary Buckwheat Shaped a Regulatory Module for Seedling Salt Tolerance by Targeting the Magnesium Transporter Gene FtMGT2. Lu, Xiang,He, Yuqi,Weng, Wenfeng,Liu, Zebin,Gao, Yuanfen,Shi, Yaliang,Li, Wei,Lai, Dili,Zhao, Mengyu,Jha, Rintu,Zhao, Hui,Li, Guangsheng,Guan, Chaonan,Shao, Shuai,Ruan, Jingjun,Woo, Sun Hee,Ouyang, Yinan,Quinet, Muriel,Georgiev, Milen I.,Fernie, Alisdair R.,Hou, Congcong,Zhang, Kaixuan,Liu, Xu,Zhou, Meiliang. 2025

[13]Integrative genome-wide association study and transcriptomic analysis to identify candidate genes and pathways for heterophil and lymphocyte traits in white-feathered broilers. Jumei Zheng,Qiao Wang,John F. O'Grady,Zixuan Wang,Man Deng,Jin Zhang,Xiang Li,Yuqing Zhu,David E. MacHugh,Guiping Zhao. 2026

[14]DissectingthemaizedirectandindirectdefenseresponseagainstAsianCornBorer. 汪海,李圣彦,查象敏,朱莉,黄大昉,郎志宏. 2015

[15]TheDifferentialTranscriptionNetworkbetweenEmbryoandEndospermintheEarlyDevelopingMaizeSeed. XiaoduoLu,DijunChen,DefengShu,ZhaoZhang,WeixuanWang,ChristianKlukas,Ling-lingChen,YunliuFan,MingChen,ChunyiZhang. 2015

[16]Joint-linkage mapping and GWAS reveal extensive genetic loci that regulate male inflorescence size in maize. Wu, Xun,Li, Yongxiang,Shi, Yunsu,Song, Yanchun,Zhang, Dengfeng,Li, Chunhui,Li, Yu,Wang, Tianyu,Buckler, Edward S.,Buckler, Edward S.,Zhang, Zhiwu,Wu, Xun,Zhang, Zhiwu.

[17]Numerous genetic loci identified for drought tolerance in the maize nested association mapping populations. Li, Chunhui,Li, Yongxiang,Wu, Xun,Zhang, Dengfeng,Shi, Yunsu,Song, Yanchun,Wang, Tianyu,Li, Yu,Sun, Baocheng,Liu, Cheng,Buckler, Edward S.,Buckler, Edward S.,Zhang, Zhiwu. 2016

[18]Genome-wide association studies of leaf angle in maize. Bo Peng,Xiaolei Zhao,Yi Wang,Chunhui Li,Yongxiang Li,Dengfeng Zhang,Yunsu Shi,Yanchun Song,Lei Wang,Yu Li,Tianyu Wang. 2021

[19]Epistasis Activation Contributes Substantially to Heterosis in Temperate by Tropical Maize Hybrids. Sang, Zhiqin,Wang, Hui,Yang, Yuxin,Zhang, Zhanqin,Liu, Xiaogang,Li, Zhiwei,Xu, Yunbi. 2022

[20]Integration of high-throughput phenotyping , GWAS, and predictive models reveals the genetic architecture of plant height in maize. Wang, Weixuan,Guo, Weijun,Le, Liang,Yu, Jia,Wu, Yue,Li, Dongwei,Wang, Yifan,Wang, Huan,Lu, Xiaoduo,Qiao, Hong,Gu, Xiaofeng,Tian, Jian,Zhang, Chunyi,Pu, Li. 2023

作者其他论文 更多>>