数字农科院2.0

Improved 1-Deoxynojirimycin (DNJ) production in mulberry leaves fermented by microorganism

文献类型： 外文期刊

作者： Jiang, Yun-Gang;Wang, Chu-Yan;Jin, Chao;Jia, Jun-Qiang;Guo, Xijie;Zhang, Guo-Zheng;Gui, Zhong-Zheng;Jia, Jun-Qiang;Guo, Xijie;Zhang, Guo-Zheng;Gui, Zhong-Zheng;Wang, Chu-Yan

作者机构：

关键词： mulberry;DNJ Production;microorganisms;fermentation

期刊名称： BRAZILIAN JOURNAL OF MICROBIOLOGY

ISSN： 1517-8382

年卷期： 2014 年 45 卷 2 期

页码：

收录情况： SCI

摘要： DNJ, an inhibitor of alpha-glucosidase, is used to suppress the elevation of postprandial hyperglycemia. In this study, we focus on screening an appropriate microorganism for performing fermentation to improve DNJ content in mulberry leaf. Results showed that Ganoderma lucidum was selected from 8 species and shown to be the most effective in improvement of DNJ production from mulberry leaves through fermentation. Based on single factor and three factor influence level tests by following the Plackett-Burman design, the optimum extraction yield was analyzed by response surface methodology (RSM). The extracted DNJ was determined by reverse-phase high performance liquid chromatograph equipped with fluorescence detector (HPLC-FD). The results of RSM showed that the optimal condition for mulberry fermentation was defined as pH 6.97, potassium nitrate content 0.81% and inoculums volume 2 mL. The extraction efficiency reached to 0.548% in maximum which is 2.74 fold of those in mulberry leaf.

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