数字农科院2.0

Use of an artificial diet system to study the toxicity of gut-active insecticidal compounds on larvae of the green lacewing Chrysoperla sinica

文献类型： 外文期刊

作者： Hu, Long;Romeis, Joerg;Wang, Yanan;Han, Lanzhi;Chen, Xiuping;Peng, Yufa;Romeis, Joerg

作者机构：

关键词： Environmental risk assessment;Potassium arsenate;Cry1Ab;Cry1Ac;Cry2Aa;Non-target organism;Genetically modified crops

期刊名称： BIOLOGICAL CONTROL

ISSN： 1049-9644

年卷期： 2014 年 69 卷

页码：

收录情况： SCI

摘要： A semi-liquid artificial diet was established and found to be a suitable food source for Chrysoperla sinica larvae, comparable to aphid prey. Using the artificial diet, we established and validated a dietary exposure assay by using the insecticidal potassium arsenate (PA) as positive control. Dose-dependent responses were documented for all observed life-table parameters of C. sinica larvae such as survival rate, pupation rate, larval weight, and larval development time. Thus, the dietary assay can detect the effects of insecticidal compounds on the survival and development of C. sinica larvae. Using the established dietary assay, we subsequently tested the toxicity of Cry1Ab, Cry1Ac, and Cry2Aa proteins (which are produced by transgenic maize, cotton or rice plants) to C. sinica larvae. Artificial diets containing Galanthus nivalis agglutinin (GNA) or PA were included as positive controls. Survival and development of C. sinica larvae were not affected when the artificial diet contained purified Cry1Ab, Cry1Ac, or Cry2Aa at 200 mu g/g diet. In contrast, C. sinica larvae were adversely affected when the diet contained PA and GNA. The stability and bioactivity of the Cry proteins in the diet and Cry protein uptake by the lacewing larvae were confirmed by bioassay with a Cry-sensitive insect species and by ELISA. The current study describes a suitable experimental system for assessing the potential effects of gut-active insecticidal compounds on green lacewing larvae. The experiments with the Cry proteins demonstrate that C. sinica larvae are not sensitive to Cry1Ab, Cry1Ac, and Cry2Aa. (C) 2013 Elsevier Inc. All rights reserved.

分类号：

  • 相关文献

[1]Use of a dietary exposure system for screening of insecticidal compounds for their toxicity to the planthopper Laodelphax striatellus. Wang, Zeng-Xia,Lin, Ke-Jian,Romeis, Joerg,Liu, Yong-Lei,Li, Yun-He,Peng, Yu-Fa,Wang, Zeng-Xia,Liu, Ze-Wen,Romeis, Joerg. 2014

[2]棉铃虫中肠钙粘蛋白、氨肽酶N及碱性磷酸酯酶的抗血清对Cry1Ac和Cry2Aa毒力的影响. 袁向东,赵曼,刘臣,陈琳,梁革梅. 2016

[3]亚致死剂量cry1Ab和cry1Ac毒蛋白对二化螟生长发育的影响及其抗性反应. 于天海,于宏坤,吴孔明,郭予元. 2005

[4]N-乙酰半乳糖胺对cry1Ac结合棉铃虫中肠氨肽酶的调控作用研究. 吴孔明,梁革梅,张永军,郭予元. 2006

[5]Development of a Tier-1 Assay for Assessing the Toxicity of Insecticidal Substances Against Coleomegilla maculata. Chen, Mao,Liu, Xiaoxia,Shelton, Anthony M.,Ostrem, Jared,Romeis, Joerg,Hellmich, Richard L.,Hellmich, Richard L.,Li, Yunhe,Peng, Yufa.

[6]二化螟APN1的原核表达及其与Cry2Aa蛋白的结合特性研究. 刘慧,李博,牛林,邱林,王永. 2018

[7]Cry1Ab rice does not impact biological characters and functional response of Cyrtorhinus lividipennis preying on Nilaparvata lugens eggs. Yang CHEN , LAI Feng-xiang , SUN Yan-qun , HONG Li-ying , TIAN Jun-ce , ZHANG Zhi-tao , FU Qiang *. 2015

[8]The water-exiting behavior and survival of predaceous diving beetles in responses to lambda-cyhalothrin, chlorantraniliprole, and thiamethoxam. Wang L.,Liu L.,Feng S.. 2024

[9]转cry1Ab和epsps基因玉米C0030.3.5对土壤古菌丰度和多样性的影响. 王晶,王蕊,朱珂,修伟明,赵建宁,杨殿林,李刚,田秀平. 2017

[10]转Bt基因抗虫玉米对亚洲玉米螟幼虫取食行为的影响. 王振营,何康来,丛斌. 2005

[11]对Bt抗性和敏感亚洲玉米螟解毒酶和中肠蛋白酶的比较. 王振营,何康来,白树雄,张杰. 2006

[12]转cry1Ab基因粳稻对稻纵卷叶螟成虫产卵行为的影响. 刘玉娥,卢增斌,叶恭银. 2015

[13]Cry1Ab敏感和抗性亚洲玉米螟氨肽酶N基因的克隆及序列差异分析. 常雪艳,何康来,王振营,白树雄. 2011

[14]转Bt基因玉米Bt11花粉对玉米螟赤眼蜂繁殖和存活的影响. 王振营,何康来,白树雄,赵长山. 2008

[15]云南草地贪夜蛾田间种群对Bt毒素的抗性基因频率监测研究. 康强强,靳明辉,杨现明,吴孔明,李怡萍,萧玉涛. 2025

[16]Detection of genetically modified crops using multiplex asymmetric polymerase chain reaction and asymmetric hyperbranched rolling circle amplification coupled with reverse dot blot. Wang, Xiumin,Teng, Da,Guan, Qingfeng,Wang, Jianhua,Wang, Xiumin,Teng, Da,Guan, Qingfeng,Wang, Jianhua,Tian, Fang.

[17]An ultrasensitive label-free electrochemiluminescent immunosensor for measuring CrylAb level and genetically modified crops content. Gao, Hongfei,Wen, Luke,Wu, Yuhua,Wu, Gang,Gao, Hongfei,Wen, Luke,Wu, Yuhua,Wu, Gang,Fu, Zhifeng.

[18]Construction of a reference plasmid molecule containing eight targets for the detection of genetically modified crops. Wang, Xiumin,Teng, Da,Yang, Yalin,Tian, Fang,Guan, Qingfeng,Wang, Jianhua,Wang, Xiumin,Teng, Da,Yang, Yalin,Tian, Fang,Guan, Qingfeng,Wang, Jianhua.

[19]Meeting demands for increased cereal production in China. He, Zhonghu,Xia, Xianchun,Peng, Shaobing,He, Zhonghu,Lumpkin, Thomas Adam. 2014

[20]Integrative analysis identifies the safety of transgenic Cry1Ab rice to non-target spider Pardosa pseudoannulata. Bo Lv,Ying Zhou,Yuan de Peng,Zhi Wang,Qi sheng Song. 2022

作者其他论文 更多>>