Identification of sequence-related amplified polymorphism and insertion-deletion markers linked to the male fertility restorer gene of pol-like CMS06J45 in heading Chinese cabbage (Brassica rapa subsp pekinensis)
文献类型： 外文期刊
作者： Xu, X. Y.;Zhang, Y.;Zhang, L. G.;Xu, X. Y.;Fang, Z. Y.
作者机构：
关键词： BrRfp;Heading Chinese cabbage;Insertion-deletion;Sequence-related amplified polymorphism;CMS06J4
期刊名称： GENETICS AND MOLECULAR RESEARCH
ISSN： 1676-5680
年卷期： 2014 年 13 卷 4 期
页码：
收录情况： SCI
摘要： In order to map the restorer gene BrRfp of the polima (pol)-like cytoplasmic male sterility (CMS) 06J45 line in heading Chinese cabbage, an F-2 segregating population with 258 individuals of CMS06J45 and the restorer line 01S325 were tested by sequence-related amplified polymorphism (SRAP) and insertion-deletion (InDel) technologies combined with the bulked segregant analysis method. As a result, two SRAP markers, me3em3.366 and pm88bg5.263, that were linked with the BrRfp gene were identified from 463 SRAP primer pairs. By cloning, sequencing, and basic local alignment search tool analysis, the two markers were targeted to the BGIScaffold000053 of Brassica rapa in the Brassica database. Using the BGIScaffold000053 sequence, four InDel primer pairs were designed and identified to be linked with the BrRfp gene in this population. Linkage analysis showed that these markers were distributed on both sides of the BrRfp gene, the linkage distances of two nearest markers InDel878.1125 and InDel920.713 were 0.82 and 0.46 cM, respectively, and the BrRfp gene was restricted to a 243-kb genomic region of B. rapa. These specific markers provided basic information for map-based cloning of the BrRfp gene and will be very valuable for the marker-assisted selection of a new restorer line in heading Chinese cabbage.
分类号：
- 相关文献
作者其他论文 更多>>
-
The divergence of DHN‑derived melanin pathways in Metarhizium robertsii
作者：Xie, L.;Liu, Y.;Zhang, Y.;Chen, K.;Yue, Q.;Wang, C.;Dun, B.;Xu, Y.;Zhang, L.
关键词：
-
REGULATION OF IRRIGATION WATER QUALITY CAN FURTHER IMMOBILIZE CD IN CONTAMINATED SOILS
作者：LI, P.;ZHANG, Y.;LIU, D.;GUO, W.;LI, K. Y.;ZHANG, Z. L.;QI, X. B.;ZHAO, Z.J.
关键词：
-
Epigallocatechin Gallate (Egcg) Inhibited The A.lv-J-Induced Apoptosis In Df-1 C ells By Inactivation Of Nuclear Factor Kappa B Pathway
作者：Xu, Z.;Deng, M.;Li, Z.;Yang, H.;Wu, X.;Zhang, Y.
关键词：ALV-J; EGCG; NF-kappa B; DF-1 cell; apoptosis
-
Impact Of Water Allocation On S.oil Desalination And Groundwater H ydro-Chemical Characteristics: A Temporal And Spatial Analysis Of A Case Study Region In China
作者：Qi, X.;Li, P.;Zhang, Y.;Liang, Z.;Magzum, N.;Li, P.;Du, Z.;Huang, Z.
关键词：Combined well canal irrigation; Water allocation; Soil salinity; Desalinization rate; Hydro-chemical characteristics; Sodium adsorption ratio
-
Semi-Dominant Mutation In The Cysteine-Rich R.eceptor-Like Kinase Gene, Als1, C onducts Constitutive Defence Response In Rice
作者：Du, D.;Ling, Y.;Zhang, C.;He, G.;Li, Y.;Chen, X.;Zhu, M.;Xing, Y.;Liu, M.;Lu, X.;Zhang, Y.;Zhang, Q.;Sang, X.
关键词：ALS1; hypersensitive resistance (HR); CRKs; disease resistance; rice (Oryza sativa L. subsp. italica.)
-
A Microrna-1 Gene, Tci-Mir-1-3P, Is I.nvolved In Cyflumetofen Resistance B y Targeting A Glutathione S-Transferase Gene, Tcgstm4, In Tetranychus Cinnabarinus
作者：Shi, L.;Hu, J.;Feng, K.;Zhang, Y.;Wei, P.;Shen, G.;He, L.;Xu, Q.;Xu, Z.;Li, M.
关键词：T; cinnabarinus; cyflumetofen resistance; miRNA; GST gene; metabolism
-
Photosynthetic And Physiological Responses Of F.oxtail Millet (Setaria Italica L .) To Low-Light Stress During Grain-Filling Stage
作者：Wen, Y. Y.;Wang, H. F.;Yuan, X. Y.;Zhang, L. G.;Yuan, X. Y.;Guo, P. Y.;Dong, S. Q.;Huang, L.;Song, X. E.;Qi, X.
关键词：agronomic characteristics; chlorophyll content; chlorophyll fluorescence; photosynthetic physiology; yield