数字农科院2.0

Screening and analyzing genes associated with Amur tiger placental development

文献类型： 外文期刊

作者： Li, Q.;Ma, J. Z.;Zhang, M. H.;Lu, T. F.;Hu, P. F.;Sun, B.;Wang, W. J.;Wang, K. F.;Zhang, W. X.;Guan, W. J.;Ma, Y. H.;Liu, D.;Chen, J.

作者机构：

关键词： Amur tiger;Gene expression profiling;ITL-2;PLAC8;PEG10;Placenta

期刊名称： GENETICS AND MOLECULAR RESEARCH

ISSN： 1676-5680

年卷期： 2014 年 13 卷 3 期

页码：

收录情况： SCI

摘要： The Amur tiger is a unique endangered species in the world, and thus, protection of its genetic resources is extremely important. In this study, an Amur tiger placenta cDNA library was constructed using the SMART cDNA Library Construction kit. A total of 508 colonies were sequenced, in which 205 (76%) genes were annotated and mapped to 74 KEGG pathways, including 29 metabolism, 29 genetic information processing, 4 environmental information processing, 7 cell motility, and 5 organismal system pathways. Additionally, PLAC8, PEG10 and IGF-II were identified after screening genes from the expressed sequence tags, and they were associated with placental development. These findings could lay the foundation for future functional genomic studies of the Amur tiger.

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