数字农科院2.0

The Minimal Active Fragment of the Cry1Ai Toxin is Located Between 36(I) and 605(I)

文献类型： 外文期刊

作者： Lin Hui-yan;Li Ying;Shu Chang-long;Song Fu-ping;Zhang Jie

作者机构：

关键词： Bacillus thuringiensis;Cry1Ai toxin;active fragment;toxicity;Plutella xylostella

期刊名称： JOURNAL OF INTEGRATIVE AGRICULTURE

ISSN： 2095-3119

年卷期： 2014 年 13 卷 5 期

页码：

收录情况： SCI

摘要： The novel ciy1Ai gene that cloned from Bacillus thuringiensis strain SC6H8 encoded a protein exhibiting strong toxicity against Plutella xylostella and Chilo suppressalis in our previous study. Using the available information for the active fragments of other Cry toxins, eight truncated fragments were constructed to identify the minimal active fragment of Cry1Ai. All truncated fragments were expressed in Escherichia coli strain BL21 (DE3), and the insecticidal activity against 2nd-instar P. xylostella larvae was assessed using full-length Cry1Ai as a positive control. The results indicate that the minimal active fragment of the Cry1Ai toxin against P. xylostella is located between amino acid residues 36(I) and 605(I), which is smaller than the regions previously reported for Cry1A. The first two amino acids (34(T) and 35(P)) on helix alpha-1 and whole helix alpha-2 of domain I and sheet beta-32 of domain III are necessary for Cry1Ai toxin to keep its toxicity against P. xylostella.

分类号：

  • 相关文献

[1]Identification of the minimal active fragment of the Cry1Ah toxin. Zhou, Zishan,Song, Fuping,Shu, Changlong,Zhang, Jie,Huang, Dafang.

[2]The midgut cadherin-like gene is not associated with resistance to Bacillus thuringiensis toxin Cry1Ac in Plutella xylostella (L.). Guo, Zhaojiang,Kang, Shi,Zhu, Xun,Wu, Qingjun,Wang, Shaoli,Xie, Wen,Zhang, Youjun.

[3]Influences of Cry1Ac Broccoli on Larval Survival and Oviposition of Diamondback Moth. Yi, Dengxia,Cui, Shusong,Yang, Limei,Fang, Zhiyuan,Liu, Yumei,Zhuang, Mu,Zhang, Yangyong,Yi, Dengxia. 2015

[4]Proteomics-based identification of midgut proteins correlated with Cry1Ac resistance in Plutella xylostella (L.). Xia, Jixing,Guo, Zhaojiang,Yang, Zezhong,Zhu, Xun,Kang, Shi,Yang, Xin,Wu, Qingjun,Wang, Shaoli,Xie, Wen,Zhang, Youjun,Xia, Jixing,Xu, Weijun,Yang, Fengshan.

[5]High resistance of transgenic cabbage plants with a synthetic cry1Ia8 gene from Bacillus thuringiensis against two lepidopteran species under field conditions. Yi, Dengxia,Yang, Weijie,Tang, Jun,Wang, Li,Fang, Zhiyuan,Liu, Yumei,Zhuang, Mu,Zhang, Yangyong,Yang, Limei,Yi, Dengxia.

[6]Determining the involvement of two aminopeptidase Ns in the resistance of Plutella xylostella to the Bt toxin Cry1Ac: Cloning and study of in vitro function. Chang, Xiaoli,Wu, Qingjun,Wang, Shaoli,Wang, Ran,Yang, Zhongxia,Chen, Defeng,Jiao, Xiaoguo,Mao, Zhenchuan,Zhang, Youjun. 2012

[7]Down-regulation of a novel ABC transporter gene (Pxwhite) is associated with Cry1Ac resistance in the diamondback moth, Plutella xylostella (L.). Guo, Zhaojiang,Kang, Shi,Zhu, Xun,Xia, Jixing,Wu, Qingjun,Wang, Shaoli,Xie, Wen,Zhang, Youjun.

[8]Screening of cry-type promoters with strong activity and application in Cry protein encapsulation in a sigK mutant. Zheng, Qingyun,Peng, Qi,Du, Lixin,Shu, Changlong,Zhang, Jie,Song, Fuping,Zhou, Changmei.

[9]A cis-acting mutation in the pxabcg1 promoter is associated with cry1ac resistance in plutella xylostella (l.). Jianying Qin,Fan Ye,Linzheng Xu,Xuguo Zhou,Neil Crickmore,Xiaomao Zhou,Youjun Zhang,Zhaojiang Guo. 2021

[10]A versatile contribution of both aminopeptidases N and ABC transporters to Bt Cry1Ac toxicity in the diamondback moth. Dan Sun,Liuhong Zhu,Le Guo,Shaoli Wang,Qingjun Wu,Neil Crickmore,Xuguo Zhou,Alejandra Bravo,Mario Soberón,Zhaojiang Guo,Youjun Zhang. 2022

[11]Plant Polysaccharides Modulate Biofilm Formation and Insecticidal Activities of Bacillus thuringiensis Strains. Mengmeng Li,Changlong Shu,Wang Ke,Xiaoxiao Li,Yiyan Yu,Xiong Guan,Tianpei Huang. 2021

[12]A cis-acting mutation in the pxabcg1 promoter is associated with cry1ac resistance in plutella xylostella (l.). Jianying Qin,Fan Ye,Linzheng Xu,Xuguo Zhou,Neil Crickmore,Xiaomao Zhou,Youjun Zhang,Zhaojiang Guo. 2021

[13]MAPK-Activated Transcription Factor PxJun Suppresses PxABCB1 Expression and Confers Resistance to Bacillus thuringiensis Cry1Ac Toxin in Plutella xylostella (L.). Jianying Qin,Le Guo,Fan Ye,Shi Kang,Dan Sun,Liuhong Zhu,Yang Bai,Zhouqiang Cheng,Linzheng Xu,Chunzheng Ouyang,Lifeng Xiao,Shaoli Wang,Qingjun Wu,Xuguo Zhou,Neil Crickmore,Xiaomao Zhou,Zhaojiang Guo,Youjun Zhang. 2021

[14]MAP4K4 controlled transcription factor POUM1 regulates PxABCG1 expression influencing Cry1Ac resistance in Plutella xylostella (L.). Linzheng Xu,Jianying Qin,Wei Fu,Shaoli Wang,Qinjun Wu,Xuguo Zhou,Neil Crickmore,Zhaojiang Guo,Youjun Zhang. 2022

[15]Retrotransposon-mediated evolutionary rewiring of a pathogen response orchestrates a resistance phenotype in an insect host. Zhaojiang Guo,Le Guo,Yang Bai,Shi Kang,Dan Sun,Jianying Qin,Fan Ye,Shaoli Wang,Qingjun Wu,Wen Xie,Xin Yang,Neil Crickmore,Xuguo Zhou,Youjun Zhang. 2023

[16]CRISPR/Cas9-based functional characterization of PxABCB1 reveals its roles in the resistance of Plutella xylostella (L.) to Cry1Ac, abamectin and emamectin benzoate. Chun zheng OUYANG,Fan YE,Qing jun WU,Shao li WANG,Neil CRICKMORE,Xu guo ZHOU,Zhao jiang GUO,You jun ZHANG. 2023

[17]Characterization of an Ecdysone Oxidase from Plutella xylostella (L.) and Its Role in Bt Cry1Ac Resistance. Dong, Lina,Zhu, Liuhong,Cheng, Zhouqiang,Bai, Yang,Li, Peixuan,Yang, Hanchi,Tang, Shouwen,Crickmore, Neil,Zhou, Xuguo,Guo, Zhaojiang,Zhang, Youjun. 2025

[18]The role of GPI-anchored membrane-bound alkaline phosphatase in the mode of action of Bt Cry1A toxins in the diamondback moth. Dan Sun,Qiuchen Xu,Le Guo,Yang Bai,Xuping Shentu,Xiaoping Yu,Neil Crickmore,Xuguo Zhou,Alejandra Bravo,Mario Soberón,Youjun Zhang,Zhaojiang Guo. 2025

[19]Reduction of Bacillus thuringiensis Cry1Ac toxicity against Helicoverpa armigera by a soluble toxin-binding cadherin fragment. Liu, Chenxi,Wu, Kongming,Gao, Yulin,Ning, Changming,Wu, Yidong,Ning, Changming,Oppert, Brenda.

[20]Comparison and optimization of the method for Cry1Ac protoxin preparation in HD73 strain. Zhou Xue-ping,Zhou Zi-shan,Yang Su-juan,Shu Chang-long,Song Fu-ping,Zhou Xue-ping,Zhang Jie. 2015

作者其他论文 更多>>