数字农科院2.0

Construction of a shuttle vector for use in Riemerella anatipestifer

文献类型： 外文期刊

作者： Hu, Qinghai;Miao, Shuang;Ni, Xintao;Lu, Fengying;Yu, Hui;Xing, Linlin;Jiang, Pan

作者机构：

关键词： Riemerella anatipestifer;Escherichia coli;Shuttle vector;Conjugation;Complementation

期刊名称： JOURNAL OF MICROBIOLOGICAL METHODS

ISSN： 0167-7012

年卷期： 2013 年 95 卷 2 期

页码：

收录情况： SCI

摘要： Riemerella anatipestifer causes epizootic infectious disease in poultry and serious economic losses, especially to the duck industry. Four complete genome sequences of R. anatipestifer strains are now available. However, functional studies have been limited by the lack of an effective shuttle vector. In this study, we constructed a shuttle vector, pRES, which was able to transfer plasmid DNA between Escherichia coli and R. anatipestifer strains. The vector contains the putative replication origin from R. anatipestifer plasmid pRA7026 and a ColE1 on for replication in R. anatipestifer and E. coli respectively. In addition, it contains oriT for transferring the vector into R. anatipestifer by conjugation, and the putative promoter of the streptothricin resistance gene of plasmid pRA0726 for heterologous gene expression in R. anatipestifer. The vector pRES will be useful in the investigation of gene function in R. anatipestifer. (C) 2013 Elsevier B.V. All rights reserved.

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