数字农科院2.0

The Preferential Binding of a Sensory Organ Specific Odorant Binding Protein of the Alfalfa Plant Bug Adelphocoris lineolatus AlinOBP10 to Biologically Active Host Plant Volatiles

文献类型： 外文期刊

作者： Liu, Ze-Wen;Sun, Liang;Gu, Shao-Hua;Xiao, Hai-Jun;Guo, Yu-Yuan;Zhang, Yong-Jun;Zhou, Jing-Jiang

作者机构：

关键词： Adelphocoris lineolatus;Odorant-binding proteins;Fluorescence competitive binding assay;EAG recording;Y-tube olfactometer

期刊名称： JOURNAL OF CHEMICAL ECOLOGY

ISSN： 0098-0331

年卷期： 2013 年 39 卷 9 期

页码：

收录情况： SCI

摘要： Semiochemicals such as sex pheromones and plant volatiles are crucial components of insect mating systems and host plant localization. In the olfactory signal transduction pathway, odorant-binding proteins (OBPs) are important elements that function in the first step of the pathway by carrying hydrophobic semiochemicals across the sensillum lymph to the olfactory receptors (ORs). In this study, we examined the binding affinities of semiochemicals to AlinOBP10, a putative OBP from the alfalfa plant bug, Adelphocoris lineolatus, that we demonstrate is expressed mainly in sensory organs. We then characterized the biological activities of the high affinity semiochemicals by measuring their electrophysiological activities in antennae and behavioral responses in the plant bug. AlinOBP10 displayed weak binding affinities to two major putative pheromone components, hexyl butyrate and (E)-2-hexenyl butyrate. In contrast, AlinOBP10 exhibited higher binding affinities to six host plant volatiles, namely myrcene, beta-pinene, beta-ionone, 3-hexanone, (E)-2-hexenal, and 1-hexanol. The biological activities of these six putative ligands were further studied in electroantennogram recordings and Y-tube olfactometer trials. The three compounds, (E)-2-hexenal, 1-hexanol, and 3-hexanone elicited strong electrophysiological responses, but elicited distinct behaviors. While 3-hexanone was attractive to female adults, (E)-2-hexenal and 1-hexanol were significant repellents. Although a weak electrophysiological response was elicited with beta-pinene, it was a strong repellent. These results demonstrate that AlinOBP10 can interact with attractants, as well as repellents, with some specificity toward plant volatiles over sex pheromones.

分类号：

  • 相关文献

[1]The Mouthparts Enriched Odorant Binding Protein 11 of the Alfalfa Plant Bug Adelphocoris lineolatus Displays a Preferential Binding Behavior to Host Plant Secondary Metabolites. Xiao, Qiang,Sun, Liang,Wei, Yu,Ma, Xiao-Yu,Xiao, Yong,Yang, Xian-Ming,Guo, Yu-Yuan,Zhang, Yong-Jun,Zhang, Dan-Dan,Zhang, Ya-Nan. 2016

[2]GOBP1 from the variegated cutworm peridroma saucia (Hübner) (lepidoptera: Noctuidae) displays high binding affinities to the behavioral attractant (Z)-3-hexenyl acetate. Ya Lan Sun,Jun Feng Dong,Yue Qin Song,Shao Li Wang. 2021

[3]Plant Volatiles and Herbivore Induced Plant Volatiles from Chili Pepper Act as Attractant of the Aphid Parasitoid Aphelinus varipes (Hymenoptera: Aphelinidae). Ali, Muhammad Yasir,Naseem, Tayyaba,Zhang, Jinping,Pan, Mingzhen,Zhang, Feng,Liu, Tong-Xian. 2022

[4]Attraction of Telenomus remus to Egg Volatiles of Spodoptera litura and Oviposition-Induced Plant Volatiles From Tobacco (Nicotiana tabacum). Ibrahim Osman,Zhimin Wang,Hongnian Li,Ertao Li,Honglin Feng,Jiao Yin,Gemei Liang,Zhengling Liu,Dekai Ning,Kebin Li,Yonghui Xie. 2025

[5]Odorant Binding Characteristics of Three Recombinant Odorant Binding Proteins in Microplitis mediator (Hymenoptera: Braconidae). Wang, Shanning,Ren, Liyan,Ali, Abid,Zhang, Yongjun,Guo, Yuyuan,Li, Keming,Zhang, Kang,Zhou, Jingjiang.

[6]Involvement of Holotrichia parallela odorant-binding protein 3 in the localization of oviposition sites. Er Tao Li,Han Jia Wu,Jian Hui Qin,Jing Luo,Ke Bin Li,Ya Zhong Cao,Shuai Zhang,Yu Peng,Jiao Yin. 2023

[7]Molecular and Functional Characterization of Three General Odorant-Binding Protein 2 Genes in Cydia pomonella (Lepidoptera: Tortricidae). Yanan Zhou,Cong Huang,Guanjun Fu,Rui Tang,Nianwan Yang,Wanxue Liu,Wanqiang Qian,Fanghao Wan. 2024

[8]Key amino acids in odorant-binding protein OBP7 enable Bradysia odoriphaga to recognize host plant volatiles. Yuting Yang,Shunyang Tan,Qinli Wang,Fu Wang,Youjun Zhang. 2025

[9]The antenna-specific odorant-binding protein AlinOBP13 of the alfalfa plant bug Adelphocoris lineolatus is expressed specifically in basiconic sensilla and has high binding affinity to terpenoids. Liu, Z-W.,Sun, L.,Xiao, H-J.,Gu, S-H.,Guo, Y-Y.,Zhang, Y-J.,Xiao, H-J.,Zhou, J-J.. 2014

[10]Functional analysis of female-biased odorant binding protein 6 for volatile and nonvolatile host compounds in Adelphocoris lineolatus (Goeze). Sun, L.,Wang, Q.,Wang, Q.,Khashaveh, A.,Zhang, Y. -J.,Guo, Y. -Y.,Wang, Q.,Yang, S.,Zhang, Z..

[11]Performance of Three Adelphocoris spp. (Hemiptera: Miridae) on Flowering and Non-flowering Cotton and Alfalfa. Pan Hong-sheng,Liu Bing,Lu Yan-hui,Liang Ge-mei. 2014

[12]Specific involvement of two amino acid residues in cis-nerolidol binding to odorant-binding protein 5 AlinOBP5 in the alfalfa plant bug, Adelphocoris lineolatus (Goeze). Gu, S. -H.,Guo, Y. -Y.,Zhang, Y. -J.,Han, L.,Zhou, J. -J.. 2013

[13]Comparative flight performance of three important pest Adelphocoris species of Bt cotton in China. Wu, K. M.,Guo, Y. Y.,Wyckhuys, K. A. G..

[14]Identification and characterization of the distinct expression profiles of candidate chemosensory membrane proteins in the antennal transcriptome of Adelphocoris lineolatus (Goeze). Ma, X. -Y.,Liu, Z. -W.,Xiao, Y.,Sun, L.,Ma, X. -Y.,Dong, K.,Liu, H. -W.,Wang, Q.,Guo, Y. -Y.,Zhang, Y. -J.,Sun, L..

[15]Comparative study of temperature-dependent life histories of three economically important Adelphocoris spp.. Wu, Kongming,Guo, Yuyuan,Wyckhuys, Krisa G.. 2009

[16]Identification And Characterization Of Odorant B.inding Proteins In The F orelegs Of Adelphocoris Lineolatus (Goeze). Sun, L, Wang, Q, Wang, Q, Dong, K, Xiao, Y, Zhang, YJ. 2017

[17]Silencing Of Odorant Binding Protein G.ene Alinobp4 By Rnai I nduces Declining Electrophysiological Responses Of Adelphocoris Lineolatus To Six Semiochemicals. Gu, Shao-Hua,Zhang, Xue-Ying,Wang, Song-Ying,Guo, Yu-Yuan,Zhang, Yong-Jun,Zhou, Yan-Le,Zhu, Xiao-Qiang. 2017

[18]OBP14 (Odorant-binding protein) sensing in Adelphocoris lineolatus based on peptide nucleic acid and graphene oxide. Tian Wenhua,Zhang Tao,Gu Shaohua,Guo Yuyuan,Gao Xiwu,Zhang Yongjun. 2021

[19]Molecular Characterization And Expression Analysis O.f Putative Odorant Carrier P roteins In Adelphocoris Lineolatus. Xiao, Y, Sun, L, Wang, Q, Zhang, Q, Gu, SH, Khashaveh, A, Liu, ZW, Zhang, YJ. 2018

[20]Host plants transfer induced regulation of the chemosensory genes repertoire in the alfalfa plant bug Adelphocoris lineolatus (Goeze). Yong Xiao,Liang Sun,Qi Wang,Xing-Kui An,Xin-Zheng Huang,Adel Khashaveh,Zhen-Yu Li,Yong-Jun Zhang. 2021

作者其他论文 更多>>