数字农科院2.0

Tandem duplication of P450 genes is involved in homoterpene biosynthesis in lima bean (Phaseolus lunatus)

文献类型： 外文期刊

作者： Li, Fengqi;Wu, Chunyan;Dewer, Youssef;Liang, Yan;Zhou, Jing-Jiang;Luo, Chen;Wang, Guirong

作者机构：

关键词： cytochrome P450 genes;homoterpene biosynthesis;gene duplication

期刊名称： PEST MANAGEMENT SCIENCE

ISSN： 1526-498X

年卷期： 2025 年

页码：

收录情况： SCIE（2025版）

摘要： BACKGROUNDThe irregular acyclic homoterpenes 4,8-dimethyl-1,3,7-nonatriene (DMNT) and 4,8,12-trimethyl-1,3,7,11-tridecatetraene (TMTT) are key volatile compounds involved in various chemical ecological interactions, particularly contributing to plant defense against phytophagous insects. However, the genomic basis and evolutionary history of homoterpene biosynthesis in legumes remain largely unexplored, limiting our understanding of their functional diversification and ecological roles.RESULTSUsing a yeast expression system, we identified two novel, tandemly duplicated cytochrome P450 genes, PlCYP82D47-like and PlCYP82D47, involved in DMNT and TMTT biosynthesis in lima bean (Phaseolus lunatus). PlCYP82D47-like catalyzes the conversion of (E)-nerolidol and (E,E)-geranyllinalool to DMNT and TMTT, respectively, while PlCYP82D47 specifically converts (E)-nerolidol to DMNT. Evolutionary analysis revealed syntenic tandem duplicates of these genes in lima bean, cotton, and Arabidopsis, suggesting a conserved genomic architecture for DMNT/TMTT biosynthesis among dicotyledonous plants and implying potential gene loss events in this genomic region. Computational modeling identified residues L324 and L505 as critical for substrate recognition by PlCYP82D47. Site-directed mutagenesis and in vitro enzymatic assays confirmed that substitutions at these residues significantly reduced catalytic activity.CONCLUSIONOur findings provide new insights into the enzymatic and evolutionary basis of homoterpene biosynthesis in legumes and offer promising targets for future metabolic engineering applications. (c) 2025 Society of Chemical Industry.

分类号：

  • 相关文献

[1]Knockdown Of Cytochrome P450 Genes G.h_D07G1197 And Gh_A13G2057 On C hromosomes D07 And A13 Reveals Their Putative Role In Enhancing Drought And Salt Stress Tolerance In Gossypium Hirsutum. Lu, Pu,Xu, Yanchao,Zhou, Zhongli,Kirungu, Joy Nyangasi,Magwanga, Richard Odongo,Magwanga, Richard Odongo,Hou, Yuqing,Agong, Stephen Gaya,Cai, Xiaoyan,Dong, Qi,Fang, Liu,Wang, Xingxing,Peng, Renhai,Wang, Kunbo. 2019

[2]The pattern of Phosphate transporter 1 genes evolutionary divergence in Glycine max L.. Fan, Chengming,Wang, Xu,Xiao, Chaowen,Jiang, Ying,Zhang, Xiaomei,Fu, Yong-Fu,Hu, Ruibo,Wang, Yahui,Zheng, Changying. 2013

[3]Molecular evolution of the rice miR395 gene family. Guddeti, S,Zhang, DC,Li, AL,Leseberg, CH,Kang, H,Li, XG,Zhai, WX,Johns, MA,Mao, L.

[4]The expression and phylogenetic analysis of four AP3-like paralogs in the stamens, carpels, and single-whorl perianth of the paleoherb Asarum caudigerum. Zhao, Yin-He,Peng, Seng,Li, Cheng-Yun,Zhao, Yin-He,Peng, Seng,Li, Cheng-Yun,Larson-Rabin, Zachary,Li, De-Zhu,Wang, Guo-Ying.

[5]Rapid evolution and complex structural organization in genomic regions harboring multiple prolamin genes in the polyploid wheat genome. Gao, Shuangcheng,Gu, Yong Qiang,Wu, Jiajie,Coleman-Derr, Devin,Huo, Naxin,Crossman, Curt,Jia, Jizeng,Zuo, Qi,Ren, Zhenglong,Anderson, Olin D.,Kong, Xiuying.

[6]Molecular evolution of two duplicated CDPK genes CPK7 and CPK12 in grass species: A case study in wheat (Tridcum aestivum L.). Geng, Shuaifeng,Tang, Lichuan,Zhang, Rongzhi,Sun, Minghui,Guo, Hanzi,Kong, Xiuying,Li, Aili,Mao, Long,Geng, Shuaifeng,Tang, Lichuan,Zhang, Rongzhi,Sun, Minghui,Guo, Hanzi,Kong, Xiuying,Li, Aili,Mao, Long,Geng, Shuaifeng,Zhao, Yongliang,Tang, Lichuan. 2011

[7]Functional opsin retrogene in nocturnal moth. Xiao, Haijun,Wu, Kongming,Xu, Pengjun,Feuda, Roberto,Lu, Bin,Graham, Robert I.. 2016

[8]Duplication of acetylcholinesterase gene in diamondback moth strains with different sensitivities to acephate. Shi, Xueyan,Song, Dunlun,Liang, Pei,Gao, Xiwu,Zhang, Youjun,Li, Jianhong,Liu, Yong,Li, Ming,Matsumura, Masaya,Sanada-Morimura, Sachiyo,Minakuchi, Chieka,Tanaka, Toshiharu,Miyata, Tadashi. 2014

[9]Gene Duplication Analysis Reveals No A.ncient Whole Genome Duplication B ut Extensive Small-Scale Duplications During Genome Evolution And Adaptation Of Schistosoma Mansoni. Wang, S,Zhu, XQ,Cai, XP. 2017

[10]A recent burst of gene duplications in Triticeae. Xiaoliang Wang,Xueqing Yan,Yiheng Hu,Liuyu Qin,Daowen Wang,Jizeng Jia,Yuannian Jiao. 2022

[11]Exploring the SiCCT Gene Family and Its Role in Heading Date in Foxtail Millet. Li, Congcong,Ma, Jian,Wang, Genping,Li, Haiquan,Wang, Hailong,Wang, Guoliang,Jiang, Yanmiao,Liu, Yanan,Liu, Guiming,Liu, Guoqing,Cheng, Ruhong,Wang, Huan,Wei, Jianhua,Yao, Lei. 2022

[12]6mA DNA Methylation on Genes in Plants Is Associated with Gene Complexity, Expression and Duplication. Yue Zhang,Qian Zhang,Xingyu Yang,Xiaofeng Gu,Jinming Chen,Tao Shi. 2023

[13]Evolution of antimicrobial cysteine-rich peptides in plants. Ma, Huizhen,Feng, Yong,Cao, Qianqian,Jia, Jing,Ali, Muhammad,Shah, Dilip,Meyers, Blake C.,He, Hai,Zhang, Yu. 2023

[14]Comparative Analysis Reveals Different Evolutionary Fates and Biological Functions in Wheat Duplicated Genes (Triticum aestivum L.). Licao Cui,Hao Cheng,Zhe Yang,Chuan Xia,Lichao Zhang,Xiuying Kong. 2023

[15]Uridine diphosphate glucosyltransferases are involved in spinosad resistance in western flower thrips Frankliniella occidentalis (Pergande). Wang J.,Wan Y.,Zhang Y.,Yuan J.,Zheng X.,Cao H.,Qian K.,Feng J.,Tang Y.,Chen S.,Zhang Y.,Zhou X.,Liang P.,Wu Q.. 2024

[16]Intra-Varietal Diversity and Its Contribution to Wheat Evolution, Domestication, and Improvement in Wheat. Li, Tianbao,Kong, Chuizheng,Deng, Pingchuan,Li, Chengdao,Zhao, Guangyao,Li, Hongjie,Gao, Lifeng,Cui, Dangqun,Jia, Jizeng. 2023

[17]Genome-wide identification of cotton CrRLK1L family genes and potential function of GhCrRLK1L104 in cell elongation. Dongyun Zuo,Lijin Chen,Javaria Ashraf,Hailiang Cheng,Youping Zhang,Qiaolian Wang,Limin Lv,Shang Liu,Guoli Song. 2024

[18]Genome-Wide Identification of NAC Family Genes in Oat and Functional Characterization of AsNAC109 in Abiotic Stress Tolerance. Xu Y.,Cheng J.,Hu H.,Yan L.,Jia J.,Wu B.. 2024

[19]Molecular and Functional Characterization of Three General Odorant-Binding Protein 2 Genes in Cydia pomonella (Lepidoptera: Tortricidae). Yanan Zhou,Cong Huang,Guanjun Fu,Rui Tang,Nianwan Yang,Wanxue Liu,Wanqiang Qian,Fanghao Wan. 2024

[20]Comprehensive profiling of Bcl-2-associated athanogene (BAG) genes and their genetic potential role under cold stress in Cotton. Aamir Ali Abro,Cong Sun,Mubashir Abbas,Qiankun Liu,Zheng Jie,Yanchao Xu,Yuqing Hou,Zhongli Zhou,Rashid Iqbal,Fang Liu,Xiaoyan Cai. 2025

作者其他论文 更多>>