数字农科院2.0

Transcriptome Analysis to Identify Resistance Genes Associated With Tomato Varieties in Response to MED Bemisia tabaci (Hemiptera: Aleyrodidae)

文献类型： 外文期刊

作者： Kong, Hailong;Chen, Yuxuan;Hu, Rongli;Muhammad, Taimur;Jiang, Suwan;Chen, Zhiwei;Zhang, Xi;Fan, Zicheng;Luo, Chen;Shi, Xiaobin;Wu, Qingjun;Zhang, Youjun

作者机构：

关键词： Bemisia tabaci;plant defence response;tomato varieties;transcription factors;transcriptome analysis

期刊名称： JOURNAL OF APPLIED ENTOMOLOGY

ISSN： 0931-2048

年卷期： 2025 年

页码：

收录情况： SCIE（2025版）

摘要： Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) is a serious insect pest of tomato crops. Our previous study revealed that the tomato varieties JinLingHongYu and ShiTouDaFen are resistant and susceptible to MED B. tabaci, respectively. In this study, we focused on determining the transcriptional response of these two tomato varieties before and after infestation with B. tabaci using Illumina sequencing. A total of 629, 859, 512 clear reads were mapped to the tomato reference genome. According to the filtering threshold (log2 ratio >= 2, false discovery rate (FDR) <= 0.05), 727 differentially expressed genes (379 upregulated and 348 downregulated) were identified in the susceptible tomato variety before and after infestation, respectively, while 1060 (537 upregulated and 523 downregulated) were identified in the resistant tomato variety. The Unigenes might be involved in the tomato variety resistance genes to B. tabaci, such as receptor-like kinase, the cell-wall-modifying enzymes of the xyloglucan endotransglucosylase/hydrolase family, expansin, tryptophan-arginine tyrosine transcription factor, cytochrome P450, GDSL esterase/lipase, Nucleotide-binding adaptor shared by Apaf1, R proteins and CED-4 (NB-ARC) and F-box. To verify the results of the transcriptome analysis, 18 genes were randomly chosen and subjected to RT-qPCR. The RT-qPCR results were the same as those of the transcriptome analysis. The present results provide important information on B. tabaci resistance mechanisms in tomato varieties that will be useful for further studies of the molecular mechanisms of this resistance and for breeding new pest-resistant varieties.

分类号：

  • 相关文献

[1]Role of long noncoding rnas zlmstrg.11348 and uemstrg.02678 in temperature-dependent culm swelling in zizania latifolia. Zheng-Hong Wang,Ning Yan,Xi Luo,Sai-Sai Guo,Shu-Qin Xue,Jiang-Qiong Liu,Shen-Shen Zhang,Li-Wen Zheng,Jing-Ze Zhang,De-Ping Guo. 2021

[2]Rice stripe virus nonstructural protein 3 suppresses plant defence responses mediated by the MEL-SHMT1 module. Wang, Kun,Fu, Shuai,Wu, Liang,Wu, Jianxiang,Wang, Yaqin,Xu, Yi,Zhou, Xueping. 2023

[3]Examining the transcriptional response of overexpressing anthranilate synthase in the hairy roots of an important medicinal plant Catharanthus roseus by RNA-seq. Sun, Jiayi,Peebles, Christie A. M.,Manmathan, Harish,Sun, Cheng,Sun, Cheng. 2016

[4]Genetic discovery for oil production and quality in sesame. Zhang, Yanxin,Wang, Linhai,Li, Donghua,Zhu, Xiaodong,Zhu, Xiaofeng,Gao, Yuan,Zhang, Xiurong,Liu, Kunyan,Feng, Qi,Zhao, Yan,Zhao, Qiang,Li, Wenjun,Fan, Danlin,Lu, Yiqi,Zhou, Congcong,Zhu, Chuanrang,Tian, Qilin,Wen, Ziruo,Weng, Qijun,Han, Bin,Huang, Xuehui,Zhang, Xianmei,Liu, Lifeng,Tang, Xiumei,Zhong, Ruichun.

[5]Genome-wide transcriptional analysis of two soybean genotypes under dehydration and rehydration conditions. Chen, Li M.,Zhou, Xin A.,Zhou, Rong,Wang, Cheng,Sha, Ai H.,Shan, Zhi H.,Zhang, Chan J.,Qiu, De Z.,Yang, Zhong L.,Chen, Shui L.,Chen, Li M.,Zhou, Xin A.,Zhou, Rong,Wang, Cheng,Sha, Ai H.,Shan, Zhi H.,Zhang, Chan J.,Qiu, De Z.,Yang, Zhong L.,Chen, Shui L.,Chen, Li M.,Li, Wen B.,Chang, Wei,Chen, Li M.,Li, Wen B.,Chang, Wei. 2013

[6]Identification of a POU factor involved in regulating the neuron-specific expression of the gene encoding diapause hormone and pheromone biosynthesis-activating neuropeptide in Bombyx mori. Zhang, TY,Kang, L,Zhang, ZF,Xu, WH.

[7]Construction of recombinant proteins for reprogramming of endangered Luxi cattle fibroblast cells. Hu, P. F.,Guan, W. J.,Li, X. C.,Ma, Y. H..

[8]Antagonistic HLH/bHLH Transcription Factors Mediate Brassinosteroid Regulation of Cell Elongation and Plant Development in Rice and Arabidopsis. Zhang, Li-Ying,Bai, Ming-Yi,Zhu, Jia-Ying,Wang, Hao,Wang, Wenfei,Zhao, Jun,Yang, Hongjuan,Xu, Yunyuan,Lin, Wen-Hui,Chong, Kang,Wang, Zhi-Yong,Zhang, Li-Ying,Zhu, Jia-Ying,Wang, Hao,Wang, Wenfei,Zhao, Jun,Bai, Ming-Yi,Sun, Yu,Wang, Zhi-Yong,Wu, Jinxia,Zhang, Zhiguo,Sun, Xuehui,Lu, Tiegang,Kim, Soo-Hwan,Fujioka, Shozo.

[9]Expression profiles of key transcription factors involved in lipid metabolism in Beijing-You chickens. Fu, R. Q.,Liu, R. R.,Zhao, G. P.,Zheng, M. Q.,Chen, J. L.,Wen, J..

[10]Insight into the AP2/ERF transcription factor superfamily in sesame and expression profiling of DREB subfamily under drought stress. Dossa, Komivi,Wei, Xin,Li, Donghua,Zhang, Yanxin,Wang, Linhai,Yu, Jingyin,Boshou, Liao,Zhang, Xiurong,Dossa, Komivi,Fonceka, Daniel,Cisse, Ndiaga,Dossa, Komivi,Diouf, Diaga,Fonceka, Daniel. 2016

[11]Transcriptome analysis of nitrogen-starvation-responsive genes in rice. Yang, Wenzhu,Yoon, Jinmi,Choi, Heebak,An, Gynheung,Yang, Wenzhu,Yoon, Jinmi,Choi, Heebak,An, Gynheung,Yang, Wenzhu,Fan, Yunliu,Chen, Rumei,Yang, Wenzhu,Fan, Yunliu,Chen, Rumei. 2015

[12]Isolation and Characterization of an ERF Transcription Factor Gene from Cotton (Gossypium barbadense L.). Xianpeng Meng,Fuguang Li,Chuanliang Liu,Chaojun Zhang,Zhixia Wu,Yajuan Chen.

[13]Transcriptome Analysis of Stem and Globally Comparison with Other Tissues in Brassica napus. Miao, Liyun,Zhang, Libin,Raboanatahiry, Nadia,Fu, Chunhua,Li, Maoteng,Miao, Liyun,Xiang, Jun,Gan, Jianping,Li, Maoteng,Lu, Guangyuan,Zhang, Xuekun. 2016

[14]Global analysis of the Gossypium hirsutum L. Transcriptome during leaf senescence by RNA-Seq. Min Lin,Chaoyou Pang,Shuli Fan,Meizhen Song,Hengling Wei,Shuxun Yu. 2015

[15]Molecular cloning and characterization of Bombyx mori CREB gene. Song, Hongsheng,Sun, Yue,Zhang, Yang,Li, Muwang.

[16]SHALLOT-LIKE1 Is a KANADI Transcription Factor That Modulates Rice Leaf Rolling by Regulating Leaf Abaxial Cell Development. Xu, Qian,Xue, Hong-Wei,Zhang, Guang-Heng,Zhu, Xu-Dong,Qian, Qian.

[17]Commentary: Compartive Transcriptome Analysis of Raphanus sativus Tissues. Gu, Xiaofeng,Lu, Tiegang. 2016

[18]Production and transcriptional regulation of proanthocyanidin biosynthesis in forage legumes. Zhou, Meiliang,Sun, Zhanmin,Gao, Lihua,Tang, Yixiong,Wu, Yanmin,Wei, Li,Meng, Yu.

[19]Comprehensive analysis and discovery of drought-related NAC transcription factors in common bean. Jing Wu,Lanfen Wang,Shumin Wang. 2016

[20]Comparing Gene Expression Profiles Between Bt and non-Bt Rice in Response to Brown Planthopper Infestation. Fang Wang , Duo Ning , Yang Chen , Cong Dang , Nai-Shun Han , Yu'e Liu , Gong-Yin Ye *. 2015

作者其他论文 更多>>