数字农科院2.0

Detection of anti-Trichinella antibodies in serum of experimentally-infected swine by immunochromatographic strip

文献类型： 外文期刊

作者： Fu, B. Q.;Li, W. H.;Gai, W. Y.;Yao, J. X.;Qu, Z. G.;Xie, Z. Z.;Wang, Y. H.;Zhang, D. L.;Blaga, R.

作者机构：

关键词： Trichinella spiralis;Swine;Immunochromatographic strip;Antibody detection;Diagnosis

期刊名称： VETERINARY PARASITOLOGY

ISSN： 0304-4017

年卷期： 2013 年 194 卷 2-4 期

页码：

收录情况： SCI

摘要： An immunochromatographic strip method, developed with the excretory-secretory antigens from muscle larvae (ML) of Trichinella spiralis labeled with colloidal gold, was used for the detection of anti-Trichinella antibodies in serum of experimentally-infected swine. Sera from swine infected with 200, 2000 and 20,000 infective ML were collected at different days post infection (dpi) and used to evaluate the method. The strip method was shown able to detect anti-Trichinella antibodies by 35 dpi, 28 dpi and 21 dpi for the three different infection doses, respectively, and closely correlated with the results of an ELISA test. The strip method is rapid and easy to perform and is suggested as an acceptable alternative for clinical laboratories lacking specialized equipment, and for field diagnosis of trichinellosis. (C) 2013 Elsevier B.V. All rights reserved.

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