Establishment of a Murine alpha nu beta 1 Transgenic CHO-K1 Cell Line and its Susceptibility to Foot-and-Mouth Disease Virus Type Asia 1/HN/2006 in China
文献类型: 外文期刊
作者: Zhang, Y.;Zheng, H. X.;Zhang, Z. D.;Jin, Y.;Yang, F.;He, J. J.;Cao, W. J.;Sun, D. H.;Lv, L.
作者机构:
关键词: Foot-and-mouth disease;foot-and-mouth disease virus;integrin;receptor;cellular
期刊名称: JOURNAL OF ANIMAL AND VETERINARY ADVANCES
ISSN: 1680-5593
年卷期: 2013 年 12 卷 1 期
页码:
收录情况: SCI
摘要: Field isolates of Foot-and-Mouth Disease Virus (FMDV) were found to use four alpha nu integrins (alpha nu beta 1, alpha nu beta 3, alpha nu beta 6 and alpha nu beta 8) as cellular receptors. Researchers established a stable Chinese Hamster Ovary clone K1 (CHO-K1) cell line expressing the murine alpha nu beta 1 heterodimer (designated as CHO-K1-alpha nu beta 1) using a highly efficient lentiviral-based gene transfer technology to deliver murine alpha nu, Internal Ribosome Entry Site (IRES) and beta 1 genes into cell chromosomes and the inserted genes were then transcribed from a Cytomegalovirus (CMV) promoter. alpha nu beta 1 expression was stringently regulated by Doxycycline (Dox) and was found to be stable. CHO-K1-alpha nu beta 1 cells were susceptible to FMDV type Asia 1/HN/2006. The plaque assay revealed that the virus produced bigger and more plaques in CHO-K1-alpha nu beta 1 cells (1.05 x 10(4) PFU mL(-1)) than in CHO-K1 cells. When sodium heparin (1 and 2 mg mL(-1)) was used as the inhibitor, the number of plaques in CHO-K1 cells were significantly decreased (4.0 x 10(3)-35 and 20 PFU mL(-1)), supported by time-course of replication and proliferation. The number and size of plaques on CHO-K1-alpha nu beta 1 cells showed no obvious change, indicating that the alpha nu beta 1 heterodimer expressed on CHO-K1-alpha nu beta 1 can be used as an FMDV receptor.
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