数字农科院2.0

Microbacterium neimengense sp nov., isolated from the rhizosphere of maize

文献类型： 外文期刊

作者： Gao, Miao;Zhang, Yan-chun;Xie, Lin-qi;Hu, Hai-yan;Xu, Jing;Sun, Jian-guang;Wang, Min;Zou, Xiao-ling;Gao, Jun-lian

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期刊名称： INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY

ISSN： 1466-5026

年卷期： 2013 年 63 卷

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收录情况： SCI

摘要： A Gram-positive, non-motile, non-spore-forming, rod-shaped bacterium, strain 7087(T), was isolated from rhizosphere of maize in China. The strain grew at 4-50 degrees C and at pH 4-10, with optima of 37 degrees C and pH 7.0, respectively. Phylogenetic analysis based on the full-length 16S rRNA gene sequence revealed that strain 7087(T) was a member of the genus Microbacterium. High levels of 16S rRNA gene similarities were found between strain 7087(T) and Microbacterium binotii DSM 19164(T) (99.8%). However, the DNA DNA hybridization value between strain 7087(T) and Microbacterium binotii DSM 19164(T) was 24.2%. The DNA G + C content of strain 7087(T) was 69.9 mol%. The major fatty acids were anteiso-C-17:0 (36.45%), anteiso-C-15:0 (36.08%) and iso-C-(16:0) (16.11%). The predominant menaquinones were MK-10 (28.1%), MK-11 (54.8%) and MK-12 (17.1%). The diagnostic diamino acid in the cell-wall peptidoglycan was ornithine. The major polar lipids are diphosphatidylglycerol, unknown phospholipids, an unknown glycolipid and unknown amino lipids. On the basis of these results, strain 7087(T) is considered to represent a novel species of the genus Microbacterium, for which the name Microbacterium neimengense sp. nov. is proposed. The type strain is 7087(T) (=ACCC 03008(T)=DSM 24985(T)).

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