数字农科院2.0

Identification of Novel Immunogenic Proteins in Mycoplasma capricolum subsp Capripneumoniae Strain M1601

文献类型: 外文期刊

作者: Zhao, Ping;He, Ying;Chu, Yue-feng;Gao, Peng-cheng;Zhang, Xuan;Zhang, Nian-zhang;Zhao, Hai-yan;Zhang, Ke-shan;Lu, Zhong-xin

作者机构:

关键词: homology;identification;immunoblotting;immunogenic proteins;Mycoplasma capricolum subsp Capripneumonia

期刊名称: JOURNAL OF VETERINARY MEDICAL SCIENCE

ISSN: 0916-7250

年卷期: 2012 年 74 卷 9 期

页码:

收录情况: SCI

摘要: The purpose of this study was to obtain immunogenic proteins and potential proteins of interest that were isolated from Mycoplasma capricolum subsp. capripneumoniae (Mccp) by MALDI-TOF mass spectrometry. One-dimensional SDS-PAGE and two-dimensional gel electrophoresis of whole cell preparation were conducted, and membrane proteome maps were prepared by immunoblotting. One-dimensional SDS-PAGE identified three immunogenic proteins with molecular masses in the range 29-97.2 kDa, two of which were in the membrane protein fraction. After two-dimensional gel electrophoresis, 20 highly immunogenic proteins were identified in the whole cell protein preparation while 9 immunogenic proteins were identified in the membrane protein fraction. This indicated that membrane proteins were the principle immunogenic proteins in Mccp. These proteins may have potential for the development of improved diagnostic tests and possible vaccines.

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