数字农科院2.0

Frenemy: adaptive temperate phage_SAP_1432 supports Staphylococcus aureus survival in changing temperatures

文献类型： 外文期刊

作者： Liu, Ting-ting;Gao, Peng-cheng;Cui, Jie-wen;Wang, Wu-bin;Zheng, Fu-ying;Li, Xue-rui;Chu, Yue-feng

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关键词： Staphylococcus aureus;heat-resistant temperate phage;temperature;thermal performance curve;lysogeny

期刊名称： MICROBIOLOGY SPECTRUM

ISSN：

年卷期： 2025 年

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收录情况： SCIE（2025版）

摘要： It is important to understand how the presence of pathogens could benefit the survival of their hosts during climate change. In this study, we investigated the influence of the heat-resistant temperate phage phage_SAP_1432 on its bacterial host Staphylococcus aureus Q1432 at different temperatures. Thermal host performance curves at a multiplicity of infection of 0.001 showed that the phage killed the bacterium efficiently from 20 to 39 degrees C and from 47 to 50 degrees C. However, the phage resulted in a 41.2% increase in the maximal growth rate for the host bacterium from 1.53 (95% confidence interval [CI]: 1.42-1.63) in the absence of phage_SAP_1432 to 2.16 (95% CI: 2.02-2.37) in its presence, along with a shift in the optimum temperature from 41.0 degrees C to 44.1 degrees C. In the absence of phage, the maximum survival temperature of S. aureus Q1432 was 51 degrees C, but after co-culture with phage_SAP_1432, a few bacteria survived at 55 degrees C-80 degrees C. Lysogenesis occurred more easily at lower temperatures; the percentage of lysogenesis increased with phage concentration, while the maximum growth rate of the host decreased. Our findings provide new evidence that heat-resistant temperate phages can benefit the survival of their bacterial host at specific temperatures, giving a new perspective on the effects of co-evolution of heat-resistant temperate phages and bacterial hosts in an era of global climate change.IMPORTANCEUnderstanding pathogen-host interactions is crucial for predicting climate change impacts on microbial ecosystems. This study examined the heat-resistant temperate phage_SAP_1432 and its effects on Staphylococcus aureus Q1432 at various temperatures. Phage_SAP_1432 enhanced the thermal performance and survival at high temperatures of its host at a low MOI. This mutual benefit demonstrates the adaptive advantages phages provide in changing thermal environments. As global temperatures rise, such phage-host interactions may play a critical role in microbial survival and evolution. Our research highlights the potential for phages to act as allies, offering a new perspective on the co-evolution of heat-resistant temperate phages and their bacterial hosts.

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